1. 遺伝子発現プロファイリングを用いたIL-1β刺激顎関節滑膜細胞に対する COX阻害薬の抗炎症効果の解析
- Author
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河 島 睦, 倉 直 美, 矢 野 照 雄, 石 上 大 輔, 渡 邊 駿, 服 部 俊 夫, 福 里 英 彦, 伊 藤 耕, and 近 藤 壽 郎
- Subjects
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TEMPOROMANDIBULAR joint , *ANTI-inflammatory agents , *GENE expression , *CELL culture , *CELECOXIB - Abstract
BACKGROUND：Non-steroidal anti-inflammatory drugs (NSAIDs) have been widely used for the management of pain and inflammation. However, little remains known about the effects of NSAIDs on synovitis of the human temporomandibular joint (TMJ). Cyclooxygenase (COX) inhibitor are one of main NSAIDs. This study investigate the effects of COX inhibitor on fibroblast-like synoviocytes (FLS) derived from the human TMJ. METHODS：Human synovial tissue was obtained from a patient with internal derangement who underwent arthroscopy of the TMJ. FLSs were prepared from the tissues using the outgrowth method. A COX inhibitor (indometacin or celecoxib) was added to the IL-1β stimulating cells in culture. Gene expression profiling was performed using microarray analysis. RESULTS：The expressions of IL-23A, IL-11, and IL-33, which were up-regulated in FLSs stimulated by IL-1xxxwere down-regulated in the cells treated by COX inhibitors. CONCLUSION：COX inhibitors such as indomethacin and celecoxib reduce the gene expression of inflammatory cytokines induced by IL-1β Our results suggest that these COX inhibitors are useful for treating synovitis in TMJ. [ABSTRACT FROM AUTHOR]
- Published
- 2019