1. Identification of oral yeasts by polyacrylamide gel electrophoresis.
- Author
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Maiden, M. F. J. and Tanner, A.
- Subjects
- *
YEAST , *POLYACRYLAMIDE gel electrophoresis , *PROTEINS , *ELECTROPHORESIS , *ACRYLAMIDE , *SACCHAROMYCES cerevisiae - Abstract
Protein profiles of sonicated cells for 9 species of yeasts isolated from oral samples were obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and visualized with a silver stain. The profiles obtained on 12% acrylamide gels we re distinct, and characteristic for 6 species of Candida, and Torulopsis glabrata, Yarrowia lipolytica, and Saccharomyces cerevisiae. Using this method, 79 fresh isolates of yeasts from saliva samples were identified; 58 as Candida albicans, 9 as Candida parapsilosis, 1 as Candida tropicalis, and 11 as T. glabrata. SDS-PAGE offers a rapid, convenient alternative or adjunct to yeast identification systems based on carbohydrate assimilation tests. [ABSTRACT FROM AUTHOR]
- Published
- 1991
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