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Oral Diseases (2011) 18, 55-59 Aim: We aimed to investigate a possible association of the MCP-1 and CCR2 polymorphisms with the risk of developing oral squamous cell carcinoma (OSCC). Methods: MCP-1 A2518G and CCR2 V64I gene polymorphisms were performed by polymerase chain reaction and restriction fragment length polymorphism, in 129 patients with OSCC and 140 healthy control subjects. Results: Individuals who had G allele and GG genotype of MCP-1, and 64I allele and wt/64I genotype of CCR2 had increased risk for OSCC (P < 0.05.) In contrast, individuals with CCR2 wt/wt genotype seem to be protected from OSCC ( P < 0.01). Haplotype analysis revealed that MCP-1G: CCR2 64I haplotype frequencies were significantly higher in patients than those of controls (P = 0.001). Conclusions: We can suggest that the G allele of MCP-1 and 64I allele of CCR2 may be risk factors for OSCC. [ABSTRACT FROM AUTHOR]

Oral Diseases (2010) , 60-67 To investigate the levels of antimicrobial peptide hCAP-18/LL-37 protein and mRNA expression in gingival tissues with different periodontal disease. Ten patients with generalized aggressive periodontitis, 10 patients with chronic periodontitis, and 10 healthy controls were included in this study. Periodontal parameters including probing depth, clinical attachment level, plaque index, and papilla bleeding index were assessed in study subjects. hCAP-18/LL-37 mRNA analysis by RT-PCR and immunohistochemistry were performed in 19 samples provided enough RNA in terms of concentration and integrity. This study demonstrated that hCAP-18/LL-37 was a product of neutrophils. Tissue samples of chronic periodontitis patients had significantly higher immunostaining of hCAP-18/LL-37 on neutrophils infiltrating in both epithelium and connective tissue compared with controls. hCAP-18/LL-37 mRNA expression levels in tissue samples of chronic periodontitis patients seemed to be upregulated compared with controls. While two generalized aggressive periodontitis patients showed downregulated hCAP-18/LL-37 mRNA expression levels, one generalized aggressive periodontitis patient showed slightly increased hCAP-18/LL-37 mRNA level compared with controls. hCAP-18/LL-37 has an important role in innate response during periodontal inflammation. Local deficiency in hCAP-18/LL-37 might be a confounding effect in the pathogenesis of generalized aggressive periodontitis. [ABSTRACT FROM AUTHOR]

Oral Diseases (2010) , 68-76 To investigate possible relationship between smoking and serum concentrations of matrix metalloproteinase-8,-9 (MMP-8, MMP-9), tissue inhibitor of matrix metalloproteinases-1 (TIMP-1), neutrophil elastase (NE), myeloperoxidase (MPO) in chronic periodontitis (CP) patients relative to periodontally healthy subjects. Serum samples were obtained from 111 subjects before initiation of any periodontal intervention. Fifty-five CP patients (39 non-smokers, 16 smokers) and 56 periodontally healthy subjects (39 non-smokers, 17 smokers) were recruited. Serum concentrations of MMP-8 were determined by IFMA and MPO, MMP-9, TIMP-1, NE concentrations by ELISA. ANCOVA and Pearson correlation analysis was utilized for statistical analysis. Serum MPO, NE concentrations were higher in smoker CP than non-smoker CP patients ( P = 0.002 and P < 0.001, respectively), whereas these were similar in smoker, non-smoker periodontally healthy groups ( P > 0.05). TIMP-1 concentration was higher in non-smoker CP than smoker CP group ( P < 0.05). MMP-9/TIMP-1 ratios were higher in smoker CP than non-smoker CP group ( P = 0.01). MMP-8 concentrations, MMP-8/TIMP-1 and MMP-9/TIMP-1 ratios in CP group were not significantly different from those in periodontally healthy group ( P > 0.05). Our findings of significantly elevated serum MMP-9, MPO, NE together with decreased TIMP-1 in smoker CP patients than non-smokers support that smoking together with periodontal destruction may expose/predispose to cardiovascular diseases. [ABSTRACT FROM AUTHOR]