1. Raman sorting and identification of single living micro-organisms with optical tweezers
- Author
-
De Chen, Yong-qing Li, and Changan Xie
- Subjects
Materials science ,Spectrophotometry, Infrared ,Cell Separation ,Spectrum Analysis, Raman ,Staining technique ,Specimen Handling ,law.invention ,Micromanipulation ,symbols.namesake ,Optics ,Food particles ,law ,Physical Stimulation ,Microchannel ,Bacteria ,business.industry ,Lasers ,Sorting ,Equipment Design ,Laser ,Microspheres ,Atomic and Molecular Physics, and Optics ,Equipment Failure Analysis ,Optical tweezers ,symbols ,business ,Raman spectroscopy ,Raman scattering - Abstract
We report on a novel technique for sorting and identification of single biological cells and food-borne bacteria based on laser tweezers and Raman spectroscopy (LTRS). With this technique, biological cells of different physiological states in a sample chamber were identified by their Raman spectral signatures and then they were selectively manipulated into a clean collection chamber with optical tweezers through a microchannel. As an example, we sorted the live and dead yeast cells into the collection chamber and validated this with a standard staining technique. We also demonstrated that bacteria existing in spoiled foods could be discriminated from a variety of food particles based on their characteristic Raman spectra and then isolated with laser manipulation. This label-free LTRS sorting technique may find broad applications in microbiology and rapid examination of food-borne diseases.
- Published
- 2005