4 results on '"Mao CX"'
Search Results
2. The molecular classification of astrocytic tumors.
- Author
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Mao CX, Yin JY, Zhang Y, Wang ZB, Yang ZQ, He ZW, Li XM, Mao XY, Cui RT, Li XJ, Li X, Zhang W, Zhou HH, and Liu ZQ
- Abstract
Aim: This study will explore the genetic and epigenetic alterations in astrocytomas, and identify the critical molecular signatures and signaling pathways for prognosis assessment by multiplatform comprehensive analysis., Method: We performed integration analyses of incorporating DNA methylation, mRNA expression, microRNA expression, and long non-coding RNA (lncRNA) expression in 33 astrocytic tumor tissues and 9 non-tumor brain tissues., Result: We observed that 11,795 DNA methylation sites, 3,627 genes, 136 microRNAs, and 3,334 lncRNAs were significantly differential between tumors and non-tumor brain tissues, and the filtered signatures through comprehensive analysis were significantly enriched in calcium signaling pathway. Furthermore, four signatures involved in calcium signaling pathway and age could contribute to predicting the patients' overall survival. Additionally, we identified differentially expressed signatures between IDH-mutated and IDH wild-type astrocytic tumors, and complement and coagulation cascades pathway was the most significant pathway in functional enrichment analysis using multiplatform data. The IDH wild-type astrocytomas were divided into two subtypes by Cluster of Cluster (CoC) analysis, one of which was enriched for astrocytomas overexpressed in chemokine signaling pathway., Conclusion: The calcium signaling pathway played a key role in astrocytoma tumorigenesis and prognosis. IDH mutation was a vital biomarker, and resulted in the change of expression level in complement and coagulation cascades pathway. The chemokine signaling pathway could characterize subtypes of IDH wild-type astrocytomas., Competing Interests: CONFLICTS OF INTEREST The authors declare no conflicts of interest.
- Published
- 2017
- Full Text
- View/download PDF
3. COL3A1 and SNAP91: novel glioblastoma markers with diagnostic and prognostic value.
- Author
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Gao YF, Mao XY, Zhu T, Mao CX, Liu ZX, Wang ZB, Li L, Li X, Yin JY, Zhang W, Zhou HH, and Liu ZQ
- Subjects
- Adult, Female, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Gene Ontology, Humans, Kaplan-Meier Estimate, Male, Middle Aged, Prognosis, Biomarkers, Tumor genetics, Brain Neoplasms genetics, Collagen Type III genetics, Glioblastoma genetics, Monomeric Clathrin Assembly Proteins genetics
- Abstract
Although patients with glioblastoma (GBM) have grave prognosis, significant variability in patient outcome is observed. This study aims to identify novel targets for GBM diagnosis and therapy. Microarray data (GSE4290, GSE7696, and GSE4412) obtained from the Gene Expression Omnibus was used to identify the differentially expressed genes (DEGs) by significant analysis of microarray (SAM). Intersection of the identified DEGs for each profile revealed 46 DEGs in GBM. A subset of common DEGs were validated by real-time reverse transcription quantitative PCR (qPCR). The prognostic value of some of the markers was also studied. We determined that RRM2 and COL3A1 were increased and directly correlated with glioma grade, while SH3GL2 and SNAP91 were decreased in GBM and inversely correlated with glioma grade. Kaplan-Meir analysis of GSE7696 revealed that COL3A1 and SNAP91 correlated with survival, suggesting that COL3A1 and SNAP91 may be suitable biomarkers for diagnostic or therapeutic strategies for GBM.
- Published
- 2016
- Full Text
- View/download PDF
4. MicroRNA-138 acts as a tumor suppressor in non small cell lung cancer via targeting YAP1.
- Author
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Xiao L, Zhou H, Li XP, Chen J, Fang C, Mao CX, Cui JJ, Zhang W, Zhou HH, Yin JY, and Liu ZQ
- Subjects
- A549 Cells, Adaptor Proteins, Signal Transducing genetics, Aged, Carcinoma, Non-Small-Cell Lung genetics, Cell Differentiation, Cell Line, Tumor, Cell Movement, Computational Biology, Female, Humans, Lung Neoplasms genetics, Male, MicroRNAs genetics, Middle Aged, Neoplasm Invasiveness, Neoplasm Metastasis, Phosphoproteins genetics, Real-Time Polymerase Chain Reaction, Tissue Distribution, Transcription Factors, YAP-Signaling Proteins, Adaptor Proteins, Signal Transducing metabolism, Carcinoma, Non-Small-Cell Lung metabolism, Gene Expression Regulation, Neoplastic, Genes, Tumor Suppressor, Lung Neoplasms metabolism, MicroRNAs metabolism, Phosphoproteins metabolism
- Abstract
MicroRNA (miR)-138 was found to have suppressive effects on the growth and metastasis of different human cancers. In this study, we aimed to investigate the regulatory mechanism of miR-138 in non-small cell lung cancer (NSCLC). We applied the Quantitative real-time PCR (qRT-PCR) to detect the miR-138 levels in NSCLC tissues (n=21) and cell lines, Bioinformatical predication, luciferase reporter assay and western blot to identify the target gene of miR-138. We also applied Cell transfection, MTT, transwell, and wound healing assays to reveal the role of miR-138 in NSCLC cell proliferation and malignant transformation. We observed that miR-138 expression level was significantly decreased in NSCLC tissues compared to their matched adjacent normal tissues. It was also downregulated in tissues with poor differentiation, advanced stage or lymph nodes metastasis, as well as in several NSCLC cell lines compared to normal lung epithelial cell. We further identified YAP1 as a direct target gene of miR-138, and observed that the protein level of YAP1 was negatively mediated by miR-138 in NSCLC A549 cells. Moreover, overexpression of miR-138 significantly inhibited A549 cell growth, invasion and migration, while knockdown of miR-138 enhanced such capacities. Further investigation showed that the cell proliferation capacity was higher in the miR-138+YAP1 group, when compared with that in the miR-138 group, suggesting that overexpression of YAP1 rescued the suppressive effects of miR-138 upregulation on NSCLC cell proliferation. However, we found no difference of cell invasion and migration capacities between miR-138+YAP1 group and miR-138 group. Finally, YAP1 was markedly upregulated in NSCLC tissues compared to their marched adjacent normal tissues. Its mRNA levels were reversely correlated with the miR-138 levels in NSCLC tissues. In summary, our study suggests that miR-138 may play a suppressive role in the growth and metastasis of NSCLC cells partly at least by targeting YAP1., Competing Interests: No conflict of interest exists in this study.
- Published
- 2016
- Full Text
- View/download PDF
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