1. IL-17RC is critically required to maintain baseline A20 production to repress JNK isoform-dependent tumor-specific proliferation
- Author
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Chi Yan, Jun Wang, David W. Hoskin, Tong-Jun Lin, Averil Ma, and Yang Lei
- Subjects
Male ,0301 basic medicine ,Gerontology ,MAP Kinase Kinase 4 ,Melanoma, Experimental ,Transfection ,IL-17 receptor C ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Cell Line, Tumor ,medicine ,Animals ,Receptor ,Melanoma ,Transcription factor ,Tumor Necrosis Factor alpha-Induced Protein 3 ,Cell Proliferation ,JNK2 ,Mice, Inbred BALB C ,Receptors, Interleukin-17 ,Cell growth ,business.industry ,Interleukin-17 ,JNK1 ,Mammary Neoplasms, Experimental ,Cancer ,medicine.disease ,Isoenzymes ,Mice, Inbred C57BL ,A20 ,030104 developmental biology ,Oncology ,030220 oncology & carcinogenesis ,Cancer research ,B16 melanoma ,Female ,Interleukin 17 ,Signal transduction ,business ,Research Paper ,Signal Transduction ,Transcription Factors - Abstract
// Chi Yan 1, 2 , Yang Lei 1, 2 , Tong-Jun Lin 2, 3, 5 , David W. Hoskin 2, 4 , Averil Ma 6 and Jun Wang 1, 2, 3, 5 1 Canadian Center for Vaccinology, Halifax, Nova Scotia, Canada 2 Department of Microbiology and Immunology, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada 3 Department of Pediatrics, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada 4 Department of Pathology, Dalhousie University, Halifax, Nova Scotia, Canada 5 IWK Health Centre, Halifax, Nova Scotia, Canada 6 Department of Medicine, University of California, San Francisco, California, USA Correspondence to: Jun Wang, email: jun.wang@dal.ca Keywords: IL-17 receptor C, A20, JNK1, JNK2, B16 melanoma Received: February 04, 2017 Accepted: April 17, 2017 Published: May 11, 2017 ABSTRACT The IL-17/IL-17R axis has controversial roles in cancer, which may be explained by tumor-specific results. Here, we describe a novel molecular mechanism underlying IL-17RC-controlled tumor-specific proliferation. Triggered by IL-17RC knockdown (KD), B16 melanoma and 4T1 carcinoma cells inversely altered homeostatic tumor proliferation and tumor growth in vitro and in vivo . In contrast to the existing dogma that IL-17RC-dependent signaling activates the JNK pathway, IL-17RC KD in both tumor cell lines caused aberrant expression and activation of different JNK isoforms along with markedly diminished levels of the ubiquitin-editing enzyme A20. We demonstrated that differential up-regulation of JNK1 and JNK2 in the two tumor cell lines was responsible for the reciprocal regulation of c-Jun activity and tumor-specific proliferation. Furthermore, we showed that A20 reconstitution of IL-17RCKD clones with expression of full-length A20, but not a truncation-mutant, reversed aberrant JNK1/JNK2 activities and tumor-specific proliferation. Collectively, our study reveals a critical role of IL-17RC in maintaining baseline A20 production and a novel role of the IL-17RC-A20 axis in controlling JNK isoform-dependent tumor-specific homeostatic proliferation.
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- 2017