1. Transcriptome sequencing analysis of mono‑ADP‑ribosylation in colorectal cancer cells
- Author
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Xian Li, Ning‑Ning Zhang, Ming Xiao, Lian Yang, Ting Lin, Qing‑Shu Li, Ya‑Lan Wang, and Chuan‑Ling Wang
- Subjects
0301 basic medicine ,Cancer Research ,transcriptome sequencing ,Epigenesis, Genetic ,Histones ,03 medical and health sciences ,0302 clinical medicine ,ADP-Ribosylation ,Cell Line, Tumor ,Humans ,histone modification ,Gene Regulatory Networks ,Epigenetics ,Protein Interaction Maps ,KEGG ,differential gene expression ,Gene ,Regulation of gene expression ,biology ,Oncogene ,Gene Expression Profiling ,Molecular Sequence Annotation ,General Medicine ,Articles ,Cell cycle ,Cell biology ,Chromatin ,CRC ,mono ADP ribosylation ,Gene Expression Regulation, Neoplastic ,030104 developmental biology ,Histone ,Oncology ,030220 oncology & carcinogenesis ,Mutation ,biology.protein ,Disease Progression ,Colorectal Neoplasms ,epigenetic - Abstract
Colorectal cancer (CRC) is a global health concern. The role of epigenetics in tumors has garnered increasing interest. ADP ribosylation is an epigenetic modification that is associated with a variety of biological functions and diseases, and its association with tumor development and progression has been hypothesized. However, due to the limitations of available techniques and methods, ADP ribosylation of specific sites is difficult to determine. In previous studies, it was shown that arginine-117 of histone 3 (H3R117) in Lovo cells can be modified by mono-ADP-ribosylation. This site was mutated and Lovo cells overexpressing this mutant construct were established. In the present study, the expression of differentially expressed genes (DEGs) between untransfected Lovo cells and H3R117A Lovo cells was analyzed. A total of 58,174 DEGs were identified, of which 2,324 were significantly differentially expressed (q-value 2). Functional annotation and Kyoto Encyclopedia of Genes and Genomes pathway enrichment was used to analyze the functions and possible roles of the DEGs. The DEGs were enriched in pathways associated with metabolic process, catalytic activity, organelle and chromatin structure, and dynamics. Through this comprehensive and systematic analysis, the role of mono-ADP-ribosylation in CRC was examined, providing a foundation for future studies.
- Published
- 2019