1. Effects of Clostridium difficile toxin A on K562/A02 cell proliferation, apoptosis and multi-drug resistance
- Author
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Lina Wang, Yaming Xi, Mao-Wen Yuan, Hao Zhang, and Zhuan-Zhen Ma
- Subjects
0301 basic medicine ,Cancer Research ,Cell ,Clostridium difficile toxin A ,Flow cytometry ,03 medical and health sciences ,0302 clinical medicine ,Western blot ,medicine ,Cytotoxic T cell ,medicine.diagnostic_test ,Chemistry ,Cell growth ,leukemia ,apoptosis ,Articles ,Cell cycle ,Molecular biology ,030104 developmental biology ,medicine.anatomical_structure ,Oncology ,Apoptosis ,030220 oncology & carcinogenesis ,K562/A02 cell ,multi-drug resistance - Abstract
The aim of the present study was to investigate the cytotoxic effect and multi-drug resistance (MDR) of Clostridium difficile toxin A (TcdA) on K562/A02 cells, and understand its underlying molecular pathways. K562/A02 cells were treated with TcdA at different concentrations for 24, 48 and 72 h, and the inhibition effect and drug resistance of TcdA on K562/A02 cell proliferation was assessed by methyl thiazolyl tetrazolium colorimetric assay. Furthermore, cell cycle-apoptosis was analyzed by flow cytometry, P-glycoprotein (P-gp) expression was determined by western blot analysis and caspase-3 activity was measured using a caspase-3 activity kit. TcdA inhibited K562/A02 cell proliferation in a time- and dose-dependent manner. The inhibition rate of K562/A02 cells reached 8.76±0.76, 28.55±0.43, 47.89±0.27, 58.08±0.06 and 57.70±0.79% following treatment with 50, 100, 200, 400 and 800 ng/ml TcdA, respectively, for 24 h. K562/A02 cells in the G0/G1 phase increased and cells in the S phase decreased following treatment with TcdA (P
- Published
- 2018