1. MicroRNA-340 inhibits invasion and metastasis by downregulating ROCK1 in breast cancer cells
- Author
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Hui Xu, Niraj Maskey, Jialu Song, Dengfeng Li, Lin Fang, Chenyang Wu, Hongming Song, and Kaiyao Hua
- Subjects
0301 basic medicine ,Cancer Research ,growth ,Biology ,medicine.disease_cause ,Metastasis ,03 medical and health sciences ,breast cancer ,0302 clinical medicine ,ROCK1 ,miRNA-340 ,microRNA ,medicine ,metastasis ,Gene silencing ,Oncogene ,Cancer ,Articles ,medicine.disease ,030104 developmental biology ,Oncology ,030220 oncology & carcinogenesis ,Cancer cell ,Cancer research ,Carcinogenesis - Abstract
MicroRNAs (miRNAs/miRs) are 19–25 nucleotide-long, non-coding RNAs that regulate the expression of target genes at the post-transcriptional level. In the present study, the role of miR-340 in breast cancer (BC) was investigated. The overexpression of miR-340 significantly inhibited the proliferation, migration and invasion of human breast MDA-MB-231 cancer cells in vitro. The Rho-associated, coiled-coil containing protein kinase 1 (ROCK1) gene was identified as a target of miR-340; its expression was downregulated by overexpression of miR-340 by binding to its 3′-untranslated region. The short interfering RNA-mediated silencing of ROCK1 was also performed, which phenocopied the effects of miR-340 overexpression. An inhibitor of miR-340 was used to suppress miR-340 expression, which led to increased expression of ROCK1, thus improving the proliferation, migration and invasion of MDA-MB-231 cells. Data from the present study suggest that miR-340 inhibits MDA-MB-231 cell growth and its downregulation may lead to the progression and metastasis of BC. Thus, miR340 may act as a tumor-suppressor agent that could serve a key role in the diagnosis and therapy of BC.
- Published
- 2017