1. MMP-1/2 and TIMP-1/2 expression levels, and the levels of collagenous and elastic fibers correlate with disease progression in a hamster model of tongue cancer
- Author
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Jinhua Zheng, Ming Fang, Yudong Xu, Haixia Fan, Haixia Li, and Wenhao Jiang
- Subjects
0301 basic medicine ,collagenous fiber ,Cancer Research ,Pathology ,medicine.medical_specialty ,Hamster ,Matrix metalloproteinase ,Biology ,Extracellular matrix ,03 medical and health sciences ,hamster tongue carcinoma ,0302 clinical medicine ,Tongue Carcinoma ,medicine ,computer-assisted morphological analysis ,TIMP1 ,Oncogene ,Cancer ,matrix metalloproteinases ,Articles ,medicine.disease ,elastic fiber ,030104 developmental biology ,medicine.anatomical_structure ,Oncology ,030220 oncology & carcinogenesis ,Elastic fiber - Abstract
In the present study, the presence of extracellular matrix components, including collagenous and elastic fibers, and the expression of their key regulating enzymes, were investigated in different stages of hamster tongue carcinoma development. Immunohistochemical and computer-assisted morphological analyses were performed to quantify the staining intensity and area (integral optical density) of matrix metalloproteinase (MMP)-1 and -2, tissue inhibitors of metalloproteinase (TIMP)-1 and -2, and the extent of elastic and collagenous fibers in histological sections. MMP-1, MMP-2, TIMP-1 and TIMP-2 expression levels gradually increased with tongue cancer progression, and were associated with disease pathology staging (r=0.705, 0.633, 0.759 and 0.751, respectively). By contrast, elastic fiber levels gradually decreased with cancer progression and were negatively correlated with disease staging (r=-0.881). The levels of collagenous fibers gradually increased with cancer progression and showed a positive correlation (r=0.619). In summary, the study demonstrated that MMP1/2 and TIMP1/2 expression levels, and collagenous and elastic fiber levels were significantly correlated with disease progression in a hamster model of tongue cancer.
- Published
- 2015