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Parasites are important players in ecological communities that can shape community structure and influence ecosystem energy flow. Yet beyond their effects on hosts, parasites can also function as an important prey resource for predators. Predators that consume infectious stages in the environment can benefit from a nutrient-rich prey item while concurrently reducing transmission to downstream hosts, highlighting the broad importance of this interaction. Less clear, however, are the specific characteristics of parasites and predators that increase the likelihood of consumption. Here, we determine what combination(s) of predator and parasite morphological traits lead to high parasite consumption. We exposed the infectious stages (cercariae) of five trematode (fluke) taxa to aquatic insect predators with varying foraging strategies and morphologies. Across the 19 predator-parasite combinations tested, damselfly predators in the family Coenagrionidae were, on average, the most effective predators of cercariae, consuming between 13 and 55% of administered cercariae. Large-bodied cercariae of Ribeiroia ondatrae had the highest average vulnerability to predation, with 37-48% of cercariae consumed. The interaction between predator head width and cercariae tail size strongly influenced the probability of consumption: small-bodied predators were the most effective consumers, particularly for larger tailed parasites. Thus, the likelihood of parasite consumption depended strongly on the relative size between predator and parasite. Our study helps establish that predation on free-living parasites largely follows a broader predator-prey framework. This will help to identify which predator and parasite combinations will likely have high consumptive interactions, potentially reducing parasite transmission in natural populations.

Despite their ubiquity and considerable biomass, the roles played by parasites in aquatic food webs are still not well understood, especially those of their free-living infectious stages. For instance, cercariae, the motile larvae of parasitic flukes (trematodes) may be a key source of nutrients and energy for consumers. As cercariae clonally reproduce within the digestive-gonadal gland complex of gastropod intermediate hosts that acquire nutritionally important polyunsaturated fatty acids (PUFA) mainly from their diets (e.g., by grazing on primary producers), cercariae could transfer snail-derived PUFA if consumed. Through fatty acid (FA) analysis, we explored whether a change in the diet of parasitized hosts altered the FA profiles of both snail-only and trematode-containing snail tissue, thereby affecting their nutritional values. Freshwater snails (Stagnicola elodes) infected with Plagiorchis sp. were fed three different diets (cyanobacteria, green algae, and diatoms) that differed in nutritional quality with respect to FA profiles. While diet influenced the overall FA composition of both snail-only tissue and snail tissue containing trematodes, levels of certain PUFA (mainly omega-3) were largely unaffected. Trematode-containing snail tissue also generally contained more PUFA relative to snail-only tissue. Notably, both tissue types had far higher levels of PUFA than found in their diets. Our results suggest that freshwater snail hosts, and possibly their associated trematode parasites, could be trophic upgraders of key PUFA despite anthropogenically induced changes in algal communities that may lead to overall diminished PUFA contents. As such, cercariae-mediated trophic transfers of PUFA may play important roles in aquatic food webs.

Free-living parasite infectious stages, such as motile cercariae of trematodes (flatworms), can constitute substantial biomass within aquatic ecosystems and are frequently eaten by various consumers, potentially serving as an important source of nutrients and energy. However, quantitative data on their nutritional value (e.g., essential fatty acids [EFA]) are largely lacking. As EFA are leading indicators of nutritional quality and underpin aquatic ecosystem productivity, we performed fatty acid (FA) analysis on an aggregate of ~ 30,000 cercariae of the freshwater trematode, Ribeiroia ondatrae. Individual cercariae contained 15 ng of total FA, and considerable quantities of EFA, including eicosapentaenoic (EPA, at 0.79 ng cercaria−1) and docosahexaenoic (DHA, at 0.01 ng cercaria−1) acids. We estimated annual EFA production by R. ondatrae cercariae for a series of ponds in California to be 40.4–337.0 μg m−2 yr−1 for EPA and 0.7–6.2 μg m−2 yr−1 for DHA. To investigate viability of cercariae as prey, we also compared growth and FA profiles of dragonfly larvae (naiads of Leucorrhinia intacta) fed equivalent masses of either R. ondatrae or zooplankton (Daphnia spp.) for 5 weeks. Naiads raised on the two diets grew equally well, with no significant differences found in their EFA profiles. While zooplankton are widely recognized as a vital source of energy, and an important conduit for the movement of EFA between algae and higher trophic levels, we suggest a similar role for trematode cercariae by ‘unlocking’ EFA from the benthic environment, highlighting their potential importance as a nutrient source that supports animal health.

Free-living parasite infectious stages, such as the cercariae of trematodes (flatworms), can represent substantial biomass in aquatic ecosystems, yet their interactions with other planktonic fauna are poorly understood. Given that cercariae are consumed by various aquatic predators, sometimes even preferentially over zooplankton, their presence may decrease predation pressure on free-living organisms within similar trophic niches by serving as alternate prey. Here, we experimentally examined how the presence of cercariae (Plagiorchis sp.) affected the population dynamics of common freshwater zooplankton (Daphnia sp.) in the presence of a predator (the larval dragonfly, Leucorrhinia intacta) known to consume both. After seeding 48 mesocosms with starting populations of Daphnia, we used four treatments (12 replicates each) representing a factorial combination of the absence/presence of both cercariae and dragonfly larvae and tracked Daphnia populations over 4 weeks. We found a significant interaction between the presence of cercariae and predators on Daphnia population size. When faced with predation pressure, Daphnia reached ~ 50% higher numbers when accompanied by cercariae than without, suggesting a “protective” effect of the latter by acting as substitute prey. Within aquatic ecosystems, an abundance of trematodes may prove advantageous for zooplankton communities that share common predators, but further studies will be needed to determine how this varies depending on the predator, trematode, and zooplankton taxa involved.

Along with immune defences, many animals exhibit effective anti-parasite behaviours such as parasite avoidance and removal that influence their susceptibility to infection. Host ecology and life history influence investment into comparatively fixed defences such as innate immunity but may affect the strength of anti-parasite behaviours as well. We investigated activity levels in five different species of larval amphibian with varying life histories and ecology in control, novel food stimulus, and trematode parasite (Echinoparyphium sp.) threat conditions. There was a significant interaction of species and treatment given that American toad (Bufo americanus), wood frog (Lithobates sylvaticus), and bullfrog (Lithobates catesbeianus) tadpoles generally increased their activity when parasite infectious stages were present while grey tree frogs (Hyla versicolor) and northern leopard frogs (Lithobates pipiens) did not, even though activity was negatively related to infection. In addition, there was considerable variation among species in their susceptibility to parasitism, with infection prevalence ranging from 17% in bullfrog tadpoles to 70% in wood frogs. However, amphibian life history (larval and adult traits) was not related to parasitism or level of anti-parasite behaviour at the species level. Consequently, we suggest that future investigations include more species with a range of life history traits and also consider host ecology, particularly if conspicuous anti-parasite behaviours are more likely in amphibian species that experience a relatively low risk of predation.

Population density and infection with parasites often are important factors affecting the growth and development of individuals. How these factors co-occur and interact in nature should have important consequences for individual fitness and higher-order phenomena, such as population dynamics of hosts and their interactions with other species. However, few studies have examined the joint effects of density and parasitism on host growth and development. We examined the co-influences of rearing density and parasitism, by the trematode Echinostoma trivolvis, on the growth and development of larval frogs, Rana (=Lithobates) pipiens. We also examined the potential role of parasite-mediated intraspecific competition by observing how unparasitized individuals performed when housed with other unparasitized tadpoles, versus housing with a combination of unparasitized and parasitized hosts. Mean mass and mean developmental stage were reduced under high rearing densities. The presence of parasitized conspecifics had no significant effect, but there was a significant interaction of density and parasitism presence on host mass, due to the fact that parasitized conspecifics grew poorly at high densities. Unparasitized individuals reared with parasitized and unparasitized conspecifics fared no better than unparasitized individuals reared only with one another. This result indicates that infected hosts compete as much as uninfected hosts for resources, even though infected individuals have reduced mass under high-density conditions. Resource acquisition and resource allocation are different processes, and parasitism, if it only affects the latter, might not have a discernible impact on competitive interactions.