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7 results on '"De Moreno de Leblanc A"'

5. Protective effect of the riboflavin-overproducing strain Lactobacillus plantarum CRL2130 on intestinal mucositis in mice

Author

Alejandra de Moreno de LeBlanc, Graciela Savoy de Giori, Jean Guy LeBlanc, and Romina Levit

Subjects
Mucositis, 0301 basic medicine, Otras Ingenierías y Tecnologías, Riboflavin, Endocrinology, Diabetes and Metabolism, Cell Culture Techniques, Antineoplastic Agents, INGENIERÍAS Y TECNOLOGÍAS, Protective Agents, RIBOFLAVIN, law.invention, Microbiology, Alimentos y Bebidas, Mice, 03 medical and health sciences, Probiotic, LACTIC ACID BACTERIA, law, medicine, Animals, Humans, Intestinal Mucosa, Mice, Inbred BALB C, Nutrition and Dietetics, biology, Strain (chemistry), Probiotics, food and beverages, medicine.disease, biology.organism_classification, FLUOROURACIL, Disease Models, Animal, 030104 developmental biology, 5-FLUOROURACIL, Female, Fluorouracil, MUCOSITIS, Caco-2 Cells, Lactobacillus plantarum
Abstract

Objective: Intestinal mucositis (IM), a side effect commonly associated to cancer treatments, is a local inflammatory response that causes alterations of the intestinal structure that in turn affects nutrient absorption. Lactobacillus (L.) plantarum CRL 2130 is a riboflavin-overproducing strain that has previously been shown to provide anti-inflammatory properties. The objective of this study was to evaluate the effects of this riboflavin producing strain in a chemically induced murine mucositis model. Research Methods & Procedures: Mucositis was induced by daily injections of 5-fluororacil (5-FU) after which mice were either given L. plantarum CRL 2130, L. plantarum CRL 725 (the strain from which CRL 2130 was derived that does not overproduce riboflavin), or commercial riboflavin twice daily during the 6 days of chemotherapy agent injections. The effect of the strains and riboflavin was also evaluated in vitro using Caco-2 intestinal cancer cell cultures to determine if they interfere with 5-FU´s anti-cancer activity. Results: Administration of L. plantarum CRL 2130 significantly attenuated the pathological changes induced by 5-FU in mice such as body weight loss, diarrhea, the shortening of villus height, increases in proinflammatory cytokine concentrations, and induced an elevated production of IL-10. In vitro assays using Caco-2 cells showed that the effectiveness of 5-FU was not affected by L. plantarum CRL 2130 and that this strain exerted an inhibitory mechanism against oxidative stress. Conclusion: These results indicate that the riboflavin-overproducing strain L. plantarum CRL 2130 could be useful to prevent mucositis during cancer treatments and would not affect the primary treatment. Fil: Levit, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Savoy, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina

Published
2018

6. Evaluation of immune response, microbiota, and blood markers after probiotic bacteria administration in obese mice induced by a high-fat diet

Author

Ivanna Novotny Núñez, Gabriela Perdigón, Carolina Maldonado Galdeano, and Alejandra de Moreno de LeBlanc

Subjects
Endocrinology, Diabetes and Metabolism, Mice, Obese, Gut flora, law.invention, Probiotic, fluids and secretions, law, GUT IMMUNITY, Intestine, Small, Bacteroides, Intestinal Mucosa, Mice, Inbred BALB C, Nutrition and Dietetics, biology, Microbiota, GUT MICROBIOTA, PROBIOTIC, food and beverages, FERMENTED MILK, Acquired immune system, Lacticaseibacillus casei, medicine.anatomical_structure, Liver, OBESITY, Female, Antibody, CIENCIAS NATURALES Y EXACTAS, Lactobacillus casei, Otras Ciencias Biológicas, Spleen, Diet, High-Fat, digestive system, Ciencias Biológicas, Immune system, Phagocytosis, Immunity, Weight Loss, medicine, Animals, Obesity, Immunity, Mucosal, Macrophages, Probiotics, HUMORAL IMMUNE RESPONSE, biology.organism_classification, Dietary Fats, Immunoglobulin A, Immunology, biology.protein, Bifidobacterium, Biomarkers
Abstract

Objective: Obesity is associated with alterations in intestinal microbiota and immunity. The aim of this study was to determine the effect of probiotic Lactobacillus casei CRL 431 administration on intestinal and humoral immune response, clinical parameters, and gut microbiota was evaluated using a high-fat diet to induce obesity in a mouse model. Methods: Adult mice received a conventional balanced diet or a high-fat diet supplemented with milk, milk fermented by Lactobacillus casei (FM), L. casei as suspension, or water over 60d. Histology of liver and small intestine (SI), immunoglobulin A-positive cells and macrophages in SI, phagocytic activity of spleen and peritoneal macrophages, and humoral immune response to ovalbumin were studied. Clinical parameters in serum and gut microbiota were also analyzed. Results: FM was the most effective supplement for decreasing body weight and clinical parameters in serum. The histology of liver and SI was also improved in obese mice given FM. These animals had increased numbers of immunoglobulin A-positive cells and macrophages in SI. The gut microbiota showed that obese mice given probiotics had increased Bacteroides and bifidobacteria. Administration of FM or L. casei as suspension enhanced the phagocytic activity of macrophages. The anti-ovalbumin specific immune response was not increased by any supplement assayed. Conclusion: Administration of probiotics to obese hosts improved the gut microbiota and the mucosal immunity altered by obesity, down-regulated some biochemical parameters in blood associated with metabolic syndrome, and decreased liver steatosis. These results demonstrate the potential use of probiotics in obese individuals to decrease the body weight and to improve the biochemical and immunologic parameters altered by obesity. Fil: Novotny Núñez, Ivanna. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Maldonado Galdeano, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina

Published
2014

7. Lactobacillus casei CRL 431 administration decreases inflammatory cytokines in a diet-induced obese mouse model.

Author

Núñez, Ivanna Novotny, Maldonado Galdeano, Carolina, de Moreno de LeBlanc, Alejandra, and Perdigón, Gabriela

Subjects
*OBESITY, *OBESITY complications, *ANIMAL experimentation, *CELL culture, *CHRONIC diseases, *CULTURED milk, *CYTOKINES, *DIET, *INFLAMMATION, *LACTOBACILLUS, *MICE, *NUTRITION, *STATISTICS, *DATA analysis, *DIAGNOSIS
Abstract

Objectives: Obesity is a chronic disease associated with an inflammatory process in which cytokines play an important role. Probiotic microorganisms have been associated with modulation of the host immune system. The aim of this study was to evaluate the influence of the probiotic bacterium Lactobacillus casei CRL 431 on the cytokine response in a model of mice under high-fat diet (HFD) conditions. Methods: BALB/c mice received a conventional balanced diet or an HFD. The test groups received milk, milk fermented by L casei (FM), or L casei as suspension in the drinking water. Proinflammatory and regulatory cytokine producer cells were evaluated in the small intestine and liver; the cytokine levels in the intestinal fluids were also evaluated. The percentages of immune cells as macrophages (F4/80), NKT, CD4+, CD8+ populations were determined in the liver. Adipocytes were also isolated and cultured to evaluate cytokines and the chemokine monocyte chemo-attractant protein (MCP)-1 produced by them. Results: The administration of probiotic L casei CRL 431 exerted an anti-inflammatory response in mice fed an HFD, evidenced mainly by decreasing proinflammatory cytokines, such as interleukin (IL)-6, IL-17, and tumor necrosis factor-α. Probiotic administration also was associated with fewer immune-infiltrating cells in the liver of mice that received the HFD and decreased secretion of MCP-1 by the adipocytes. This last observation could be associated with less macrophage accumulation in the adipose tissues, which is characteristic in the obese host and contributes to maintaining the inflammatory response in this organ. The results obtained show an anti-inflammatory effect of L casei CRL 431 when it is administered as a supplement of the HFD in a mouse model. [ABSTRACT FROM AUTHOR]

Published
2015
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