1. Immunomodulatory and Antioxidant Properties of Wheat Gluten Protein Hydrolysates in Human Peripheral Blood Mononuclear Cells
- Author
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Patricia J. Lardone, Justo Pedroche, Ivan Cruz-Chamorro, Guillermo Santos-Sánchez, Juan M. Guerrero, Francisco Millán, Antonio Carrillo-Vico, Ignacio Bejarano, Nuria Álvarez-Sánchez, María del Carmen Millán-Linares, María-Soledad Fernández-Pachón, [Cruz-Chamorro,I, Álvarez-Sánchez,N, Santos-Sánchez,G, Lardone,PJ, Bejarano,I, Guerrero,JM, Carrillo-Vico,A] Instituto de Biomedicina de Sevilla, IBiS (Universidad de Sevilla, HUVR, Junta de Andalucía, CSIC), Seville, Spain. [Cruz-Chamorro,I, Carrillo-Vico,A] Departamento de Bioquímica Médica y Biología Molecular e Inmunología, Universidad de Sevilla, Seville, Spain. [Pedroche,J, Millán,F] Plant Protein Group, Instituto de la Grasa, CSIC, Seville, Spain. [Fernández-Pachón,MS] Área de Nutrición y Bromatología, Departamento de Biología Molecular e Ingeniería Bioquímica, Universidad Pablo de Olavide, Sevilla, Spain. [Millán-Linares,MC] Cell Biology Unit, Instituto de la Grasa, CSIC, Seville, Spain. [Guerrero,JM] Departamento de Bioquímica Clínica, Unidad de Gestión de Laboratorios, Hospital Universitario Virgen del Rocío, Seville, Spain., This work was supported by the Spanish Government, Ministerio de Economía y Competitividad (AGL2012-40247-C02-01 and AGL2012-40247-C02-02) the Andalusian Government Ministry of Health (PC-0111-2016-0111) and the PAIDI Program from the Andalusian Government (CTS160). I.C.-C. was supported by Ministerio de Economía y Competitividad (AGL2012-40247-C02-02) and by a FPU grant from the Spanish Ministerio de Educación, Cultura y Deporte (FPU13/01210). N.Á.-S. was supported by Instituto de Salud Carlos III, Ministerio de Economía y Competitividad (RD06/0013/0001 and RD12/0043/0012). G.S.-S. was supported by a FPU grant from the Spanish Ministerio de Educación, Cultura y Deporte (FPU16/02339). I.B. was supported by the VI Program of Inner Initiative for Research and Transfer of University of Seville (VI PPIT-US)., Ministerio de Economía y Competitividad (España), Ministerio de Educación, Cultura y Deporte (España), Junta de Andalucía, Universidad de Sevilla, and Instituto de Salud Carlos III
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0301 basic medicine ,Antioxidant ,Protein Hydrolysates ,Technology and Food and Beverages::Food and Beverages::Food::Foods, Specialized::Functional Food [Medical Subject Headings] ,medicine.medical_treatment ,antioxidant capacity ,Leucocitos mononucleares ,medicine.disease_cause ,pro-inflammatory cytokines ,Antioxidants ,Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings] ,Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Molecular Mechanisms of Pharmacological Action::Antioxidants [Medical Subject Headings] ,chemistry.chemical_compound ,Functional Food ,oxidative stress ,Food science ,Cells, Cultured ,chemistry.chemical_classification ,Nutrition and Dietetics ,Chemistry ,Hydrolysis ,Interleukin-17 ,Phenomena and Processes::Metabolic Phenomena::Metabolism::Hydrolysis [Medical Subject Headings] ,Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Immunologic Tests::Interferon-gamma Release Tests [Medical Subject Headings] ,Estrés oxidativo ,04 agricultural and veterinary sciences ,peripheral blood mononuclear cells ,040401 food science ,Glutathione ,Antioxidant capacity ,Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Peptides::Oligopeptides::Glutathione [Medical Subject Headings] ,Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Plant Proteins::Seed Storage Proteins::Prolamins::Glutens [Medical Subject Headings] ,Wheat gluten protein hydrolysates ,Anatomy::Cells::Blood Cells::Leukocytes::Leukocytes, Mononuclear::Lymphocytes::T-Lymphocytes::T-Lymphocyte Subsets::T-Lymphocytes, Helper-Inducer::Th2 Cells [Medical Subject Headings] ,Inflammation Mediators ,lcsh:Nutrition. Foods and food supply ,Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Membrane Proteins::Receptors, Cell Surface::Receptors, Immunologic::Receptors, Cytokine::Receptors, Interleukin::Receptors, Interleukin-17 [Medical Subject Headings] ,Antigenicity ,Glutens ,wheat gluten protein hydrolysates ,bioactive peptides ,glutathione ,Pro-inflammatory cytokines ,lcsh:TX341-641 ,Nitric Oxide ,Peripheral blood mononuclear cell ,Article ,Proinflammatory cytokine ,03 medical and health sciences ,Interferon-gamma ,Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Protein Hydrolysates [Medical Subject Headings] ,0404 agricultural biotechnology ,Th2 Cells ,Functional food ,medicine ,Humans ,Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Growth Processes::Cell Proliferation [Medical Subject Headings] ,Bioactive peptides ,Cell Proliferation ,Th1 Cells ,Glútenes ,Gluten ,Chemicals and Drugs::Inorganic Chemicals::Nitrogen Compounds::Nitrogen Oxides::Nitric Oxide [Medical Subject Headings] ,030104 developmental biology ,Oxidative stress ,Peripheral blood mononuclear cells ,Anatomy::Hemic and Immune Systems::Blood::Blood Cells::Leukocytes::Leukocytes, Mononuclear [Medical Subject Headings] ,Anatomy::Cells::Blood Cells::Leukocytes::Leukocytes, Mononuclear::Lymphocytes::T-Lymphocytes::T-Lymphocyte Subsets::T-Lymphocytes, Helper-Inducer::Th1 Cells [Medical Subject Headings] ,Leukocytes, Mononuclear ,Chemicals and Drugs::Biological Factors::Inflammation Mediators [Medical Subject Headings] ,Food Science - Abstract
Peptides from several plant food proteins not only maintain the nutritional values of the original protein and decrease the environmental impact of animal agriculture, but also exert biological activities with significant health-beneficial effects. Wheat is the most important food grain source in the world. However, negative attention on wheat-based products has arose due to the role of gluten in celiac disease. A controlled enzymatic hydrolysis could reduce the antigenicity of wheat gluten protein hydrolysates (WGPHs). Therefore, the aims of the present study were to evaluate the effects of the in vitro administration of Alcalase-generated WGPHs on the immunological and antioxidant responses of human peripheral blood mononuclear cells (PBMCs) from 39 healthy subjects. WGPH treatment reduced cell proliferation and the production of the Type 1 T helper (Th1) and Th17 pro-inflammatory cytokines IFN-γ and IL-17, respectively. WPGHs also improved the cellular anti-inflammatory microenvironment, increasing Th2/Th1 and Th2/Th17 balances. Additionally, WGPHs improved global antioxidant capacity, increased levels of the reduced form of glutathione and reduced nitric oxide production. These findings, not previously reported, highlight the beneficial capacity of these vegetable protein hydrolysates, which might represent an effective alternative in functional food generation., This work was supported by the Spanish Government, Ministerio de Economía y Competitividad (AGL2012-40247-C02-01 and AGL2012-40247-C02-02) the Andalusian Government Ministry of Health (PC-0111-2016-0111) and the PAIDI Program from the Andalusian Government (CTS160). I.C.-C. was supported by Ministerio de Economía y Competitividad (AGL2012-40247-C02-02) and by a FPU grant from the Spanish Ministerio de Educación, Cultura y Deporte (FPU13/01210). N.Á.-S. was supported by Instituto de Salud Carlos III, Ministerio de Economía y Competitividad (RD06/0013/0001 and RD12/0043/0012). G.S.-S. was supported by a FPU grant from the Spanish Ministerio de Educación, Cultura y Deporte (FPU16/02339). I.B. was supported by the VI Program of Inner Initiative for Research and Transfer of University of Seville (VI PPIT-US).
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- 2020