1. A Novel Role of Eruca sativa Mill. (Rocket) Extract: Antiplatelet (NF-κB Inhibition) and Antithrombotic Activities
- Author
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Marcelo Alarcón, Manuel Fuentes, Gilda Carrasco, Iván Palomo, and Eduardo Fuentes
- Subjects
Blood Platelets ,Platelet Aggregation ,Interleukin-1beta ,lcsh:TX341-641 ,Eruca ,Pharmacology ,antithrombotic ,antiplatelet ,Article ,Transforming Growth Factor beta1 ,functional food ,chemistry.chemical_compound ,Mice ,Fibrinolytic Agents ,Bleeding time ,Antithrombotic ,medicine ,Animals ,Humans ,Platelet ,Eruca sativa Mill ,Chemokine CCL5 ,Nutrition and Dietetics ,biology ,medicine.diagnostic_test ,platelet inflammatory mediators ,Plant Extracts ,NF-kappa B ,food and beverages ,Thrombosis ,biology.organism_classification ,Thromboxane B2 ,Adenosine Diphosphate ,Mice, Inbred C57BL ,Disease Models, Animal ,P-Selectin ,chemistry ,Biochemistry ,Brassicaceae ,rocket ,Platelet aggregation inhibitor ,Arachidonic acid ,lcsh:Nutrition. Foods and food supply ,Fibrinolytic agent ,Platelet Aggregation Inhibitors ,Food Science - Abstract
Background: Epidemiological studies have shown the prevention of cardiovascular diseases through the regular consumption of vegetables. Eruca sativa Mill., commonly known as rocket, is a leafy vegetable that has anti-inflammatory activity. However, its antiplatelet and antithrombotic activities have not been described. Methods: Eruca sativa Mill. aqueous extract (0.1 to 1 mg/mL), was evaluated on human platelets: (i) P-selectin expression by flow cytometry, (ii) platelet aggregation induced by ADP, collagen and arachidonic acid, (iii) IL-1β, TGF-β1, CCL5 and thromboxane B2 release, and (iv) activation of NF-κB and PKA by western blot. Furthermore, (v) antithrombotic activity (200 mg/kg) and (vi) bleeding time in murine models were evaluated. Results: Eruca sativa Mill. aqueous extract (0.1 to 1 mg/mL) inhibited P-selectin expression and platelet aggregation induced by ADP. The release of platelet inflammatory mediators (IL-1β, TGF-β1, CCL5 and thromboxane B2) induced by ADP was inhibited by Eruca sativa Mill. aqueous extract. Furthermore, Eruca sativa Mill. aqueous extract inhibited NF-κB activation. Finally, in murine models, Eruca sativa Mill. aqueous extract showed significant antithrombotic activity and a slight effect on bleeding time. Conclusion: Eruca sativa Mill. presents antiplatelet and antithrombotic activity.
- Published
- 2014