Search

Your search keyword '"Olins, A L"' showing total 10 results
Start Over Author "Olins, A L" Journal nucleus
10 results on '"Olins, A L"'

2. Defining the epichromatin epitope

Author

Gould, Travis J., primary, Tóth, Katalin, additional, Mücke, Norbert, additional, Langowski, Jörg, additional, Hakusui, Alexandra S., additional, Olins, Ada L., additional, and Olins, Donald E., additional

Published
2017
Full Text
View/download PDF

5. Hyperosmotic stress: in situchromatin phase separation

Author

Olins, Ada L., Gould, Travis J., Boyd, Logan, Sarg, Bettina, and Olins, Donald E.

Abstract

ABSTRACTDehydration of cells by acute hyperosmotic stress has profound effects upon cell structure and function. Interphase chromatin and mitotic chromosomes collapse (“congelation”). HL-60/S4 cells remain ~100% viable for, at least, 1 hour, exhibiting shrinkage to ~2/3 their original volume, when placed in 300mM sucrose in tissue culture medium. Fixed cells were imaged by immunostaining confocal and STED microscopy. At a “global” structural level (μm), mitotic chromosomes congeal into a residual gel with apparent (phase) separations of Ki67, CTCF, SMC2, RAD21, H1 histones and HMG proteins. At an “intermediate” level (sub-μm), radial distribution analysis of STED images revealed a most probable peak DNA density separation of ~0.16 μm, essentially unchanged by hyperosmotic stress. At a “local” structural level (~1-2 nm), in vivo crosslinking revealed essentially unchanged crosslinked products between H1, HMG and inner histones. Hyperosmotic cellular stress is discussed in terms of concepts of mitotic chromosome structure and liquid-liquid phase separation.

Published
2020
Full Text
View/download PDF

10. Lamin B receptor

Author

Olins, Ada L., primary, Rhodes, Gale, additional, Welch, David B. Mark, additional, Zwerger, Monika, additional, and Olins, Donald E., additional

Published
2010
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results