1. Novel biotin phosphoramidites with super-long tethering arms.
- Author
-
Morocho AM, Karamyshev VN, Shcherbinina OV, Malykh AG, and Polushin NN
- Subjects
- Amides chemical synthesis, Base Sequence, Biotinylation, DNA chemistry, Indicators and Reagents, Molecular Structure, Oligodeoxyribonucleotides chemistry, Phosphoric Acids chemical synthesis, Structure-Activity Relationship, Amides chemistry, Biotin, Oligodeoxyribonucleotides chemical synthesis, Phosphoric Acids chemistry
- Abstract
A number of novel biotin phosphoramidites, possessing exceptionally long and uncharged tethering arms, were synthesized from methoxyoxalamido (MOX) and succinimido (SUC) precursors. Included among these monomers is a uridine derivative with the biotin moiety attached through the 2'-position. Some of these phosphoramidites were used to make 5'-biotinylated primers, which were applied in direct sequencing of genomic DNA and capture of Sanger fragment pools.
- Published
- 2003
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