1. Fluorescencein situhybridisation of multiple probes on a single microscope slide
- Author
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Eddy Maher, Zoia Larin, Edwin M. Southern, Yumiko Ishikawa-Brush, and Mark D. Fricker
- Subjects
Chromosome paints ,Microscope ,business.industry ,Hybridization probe ,Microscope slide ,Biology ,Molecular biology ,Fluorescence ,Chromosomes ,law.invention ,Optics ,law ,Karyotyping ,In situ hybridisation ,Bone plate ,Genetics ,Silicon rubber ,Humans ,DNA Probes ,business ,In Situ Hybridization, Fluorescence - Abstract
We report a method to analyse multiple samples by fluorescence in situ hybridisation on a single glass microscope slide. Wells were formed in which independent hybridisation reactions could proceed by sealing a silicon rubber gasket to the slide. In the largest format tested, different probes were hybridised simultaneously by applying them directly from a 96-well microtitre dish which was inverted on a glass plate. This technique will increase the rate of analysis of multiple probes against a standard set of chromosomes and could also be used to analyse different karyotypes using a panel of probes such as single chromosome paints during a single operation. It should be useful for both chromosomal mapping projects and screening for chromosome abnormalities in clinical diagnostic laboratories.
- Published
- 1994