Search

Your search keyword '"Yoneki, T."' showing total 2 results
Start Over Author "Yoneki, T." Journal nucleic acids research
2 results on '"Yoneki, T."'

1. Dmc1 of Schizosaccharomyces pombe plays a role in meiotic recombination

Author

Fukushima, K., Tanaka, Y., Nabeshima, K., Yoneki, T., Tougan, T., Tanaka, S., and Nojima, H.

Abstract

We report here a Schizosaccharomyces pombe gene (dmc1+) that resembles budding yeast DMC1 in the region immediately upstream of the rad24+ gene. We showed by northern and Southern blot analysis that dmc1+ and rad24+ are co-transcribed as a bicistronic mRNA of 2.8 kb with meiotic specificity, whereas rad24+ itself is constitutively transcribed as a 1.0-kb mRNA species during meiosis. Induction of the bicistronic transcript is under the control of a meiosis-specific transcription factor, Ste11. Disruption of both dmc1+ and rad24+ had no effect on mitosis or spore formation, and dmc1Δ cells displayed no change in sensitivity to UV or γ irradiation relative to the wild type. Tetrad analysis indicated that Dmc1 is involved in meiotic recombination. Analysis of gene conversion frequencies using single and double mutants of dmc1 and rhp51 indicated that both Dmc1 and Rhp51 function in meiotic gene conversion. These observations, together with a high level of sequence identity, indicate that the dmc1+ gene of S.pombe is a structural homolog of budding yeast DMC1, sharing both similar and distinct functions in meiosis.

Published
2000

2. Comprehensive isolation of meiosis-specific genes identifies novel proteins and unusual non-coding transcripts in Schizosaccharomyces pombe.

Author

Watanabe T, Miyashita K, Saito TT, Yoneki T, Kakihara Y, Nabeshima K, Kishi YA, Shimoda C, and Nojima H

Subjects
Base Sequence, DNA, Complementary genetics, DNA, Complementary isolation & purification, Gene Library, Genes, Fungal genetics, Molecular Sequence Data, RNA, Fungal genetics, Schizosaccharomyces physiology, Sequence Homology, Nucleic Acid, Spores, Fungal genetics, Transcription, Genetic, Fungal Proteins genetics, Gene Expression Regulation, Fungal, Meiosis genetics, Schizosaccharomyces genetics
Abstract

In order to isolate meiosis-specific genes in Schizosaccharomyces pombe, we have constructed a subtracted cDNA library enriched in clones whose expression is enhanced during meiosis induced by nitrogen starvation. Using northern blot analysis, we isolated 31 kinds of clones whose expression was induced in a meiosis/sporulation-specific manner. We comprehensively named them meu after meiotic expression upregulated. The transcription of 20 meu genes was found to be dependent on the mei4(+) gene, which encodes a transcription factor required for the progression of meiosis. DNA sequencing indicated that most of the meu genes encode novel proteins. Notably, five of the meu genes harbor no apparent protein coding sequences, and the transcripts form stable hairpin structures, suggesting that they may generate non-coding RNAs or antisense RNAS: The results presented here imply that RNAs are also important for the comprehensive characterization of genomic expression.

Published
2001
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results