1. Functional signature for the recognition of specific target mRNAs by human Staufen1 protein
- Author
-
Juan Ortín, Juan Carlos Oliveros, Monica Chagoyen, and Susana de Lucas
- Subjects
AU-rich element ,Binding Sites ,Sequence Analysis, RNA ,RNA ,RNA-Binding Proteins ,RNA-binding protein ,Biology ,Molecular biology ,Cell biology ,RNA silencing ,Cytoskeletal Proteins ,HEK293 Cells ,Mutant protein ,Alu Elements ,Translational regulation ,Genetics ,Humans ,Signal recognition particle RNA ,RNA, Messenger ,Molecular Biology ,Ribonucleoprotein - Abstract
Cellular messenger RNAs (mRNAs) are associated to proteins in the form of ribonucleoprotein particles. The double-stranded RNA-binding (DRB) proteins play important roles in mRNA synthesis, modification, activity and decay. Staufen is a DRB protein involved in the localized translation of specific mRNAs during Drosophila early development. The human Staufen1 (hStau1) forms RNA granules that contain translation regulation proteins as well as cytoskeleton and motor proteins to allow the movement of the granule on microtubules, but the mechanisms of hStau1-RNA recognition are still unclear. Here we used a combination of affinity chromatography, RNAse-protection, deep-sequencing and bioinformatic analyses to identify mRNAs differentially associated to hStau1 or a mutant protein unable to bind RNA and, in this way, defined a collection of mRNAs specifically associated to wt hStau1. A common sequence signature consisting of two opposite-polarity Alu motifs was present in the hStau1-associated mRNAs and was shown to be sufficient for binding to hStau1 and hStau1-dependent stimulation of protein expression. Our results unravel how hStau1 identifies a wide spectrum of cellular target mRNAs to control their localization, expression and fate.
- Published
- 2014