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Start Over Author "Slesarev, A. I." Journal nucleic acids research
3 results on '"Slesarev, A. I."'

3. Archaebacterial reverse gyrase cleavage-site specificity is similar to that of eubacterial DNA topoisomerases I.

Author

Kovalsky OI, Kozyavkin SA, and Slesarev AI

Subjects
Base Sequence, Densitometry, Electrophoresis, Polyacrylamide Gel, Escherichia coli enzymology, Micrococcus enzymology, Molecular Sequence Data, Substrate Specificity, Temperature, Archaea enzymology, Bacteria enzymology, DNA Topoisomerases, Type I metabolism, DNA Topoisomerases, Type II metabolism, DNA, Bacterial metabolism
Abstract

ATP-dependent type I topoisomerases from extremely thermophilic archaebacteria--reverse gyrases--drive positive supercoiling of DNA. We showed that reverse gyrase from Desulfurococcus amylolyticus breaks the DNA at specific sites and covalently binds to the 5' end. In 30 out of 31 sites located in pBR322 DNA fragments, cleavage occurs at the sequence 5'---CNNN/---(N is any base). The same rule was previously shown to hold for single-stranded DNA breakage by eubacterial topoisomerases I. The relative cleavage frequencies at different sites depend on Mg2+ and temperature. We discuss the possible physiological and mechanistic role of the above specificity of the bacterial topoisomerases I.

Published
1990
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