1. Characterisation of P. falciparum antigenic determinants isolated from a genomic expression library by differential antibody screening
- Author
-
Odile Puijalon, Bernard Kahane, Michael Koenen, Lisa Sibilli, Luis Pereira da Silva, Benno Müller−Hill, Micheline Guillotte, Ian Garner, Denise Mattei, Gordon Langsley, Artur Scherf, Institut Pasteur [Paris] (IP), Universität zu Köln = University of Cologne, and Institut Pasteur [Paris]
- Subjects
MESH: Epitopes ,030231 tropical medicine ,Plasmodium falciparum ,Clone (cell biology) ,Fluorescent Antibody Technique ,MESH: Amino Acid Sequence ,Biology ,MESH: Base Sequence ,Epitope ,Antibodies ,MESH: Nucleic Acid Hybridization ,03 medical and health sciences ,Nucleic acid thermodynamics ,Epitopes ,0302 clinical medicine ,Antigen ,Genetics ,Animals ,MESH: Animals ,MESH: Cloning, Molecular ,Amino Acid Sequence ,Cloning, Molecular ,Peptide sequence ,Gene ,MESH: Fluorescent Antibody Technique ,ComputingMilieux_MISCELLANEOUS ,MESH: Plasmodium falciparum ,030304 developmental biology ,0303 health sciences ,Base Sequence ,MESH: Antibodies ,Nucleic Acid Hybridization ,Molecular biology ,MESH: Genes ,Open reading frame ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,Genes ,biology.protein ,Antibody - Abstract
International audience; A genomic expression library of P.falciparum has been differentially screened with a number of immune sera. The response of 9 clones to the various sera is presented, together with the DNA sequence encoding the epitopes. All but one clone are extremely A+T rich and unlike the other P.falciparum epitopes described, are not composed of amino acid repeats. One clone, which responds specifically with a protective serum, has been analysed in detail. The epitope is carried on a 160kd antigen which is transcribed from a single gene to give a protein expressed in all of the erythrocytic forms. DNA sequence of this clone reveals it to have more than one open reading frame, only one of which is transcribed in the blood stages. The possible significance of the other open readings frames is discussed
- Published
- 1985