1. Ex vivo intestinal permeability assay (X-IPA) for tracking barrier function dynamics.
- Author
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Bootz-Maoz H, Simon A, Del Mare-Roumani S, Bennet Y, Toister E, Romano H, Zheng D, Amidror S, Elinav E, and Yissachar N
- Subjects
- Permeability, Host Microbial Interactions, Intestinal Mucosa, Gastrointestinal Microbiome
- Abstract
The intestinal epithelial barrier facilitates homeostatic host-microbiota interactions and immunological tolerance. However, mechanistic dissections of barrier dynamics following luminal stimulation pose a substantial challenge. Here, we describe an ex vivo intestinal permeability assay, X-IPA, for quantitative analysis of gut permeability dynamics at the whole-tissue level. We demonstrate that specific gut microbes and metabolites induce rapid, dose-dependent increases to gut permeability, thus providing a powerful approach for precise investigation of barrier functions., (© 2023. The Author(s).)
- Published
- 2023
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