1. Blood Transcriptome Analysis of Septic Patients Reveals a Long Non-Coding Alu -RNA in the Complement C5a Receptor 1 Gene.
- Author
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Emblem, Åse, Knutsen, Erik, Jørgensen, Tor Erik, Fure, Hilde, Johansen, Steinar Daae, Brekke, Ole-Lars, Mollnes, Tom Eirik, and Karlsen, Bård Ove
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COMPLEMENT (Immunology) , *BLOOD testing , *COMPLEMENT receptors , *LINCRNA , *COMPLEMENT activation , *HUMAN genome - Abstract
Many severe inflammation conditions are complement-dependent with the complement component C5a-C5aR1 axis as an important driver. At the RNA level, the blood transcriptome undergoes programmed expression of coding and long non-coding RNAs to combat invading microorganisms. Understanding the expression of long non-coding RNAs containing Alu elements in inflammation is important for reconstructing cell fate trajectories leading to severe disease. We have assembled a pipeline for computation mining of new Alu-containing long non-coding RNAs by intersecting immune genes with known Alu coordinates in the human genome. By applying the pipeline to patient bulk RNA-seq data with sepsis, we found immune genes containing 48 Alu insertion as robust candidates for further study. Interestingly, 1 of the 48 candidates was located within the complement system receptor gene C5aR1 and holds promise as a target for RNA therapeutics. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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