91 results on '"I, Moriyama"'
Search Results
2. [Glucose transport in human placental chorioepithelium]
- Author
-
Y, Yamada, I, Moriyama, H, Iioka, S, Akada, H, Hashimoto, T, Shimamoto, Y, Sakamoto, and M, Ichijou
- Subjects
Glucose ,Monosaccharide Transport Proteins ,Pregnancy ,Placenta ,Humans ,Biological Transport ,Female ,Chorion ,In Vitro Techniques ,Epithelium - Published
- 1993
Catalog
3. [The platelet aggregation inhibiting activity in human placenta--study on the ADP degrading activity of human placental villi]
- Author
-
H, Iioka, H, Hisanaga, S, Akada, I, Moriyama, and M, Ichijo
- Subjects
Hot Temperature ,Apyrase ,Humans ,Calcium ,Female ,Chorionic Villi ,Hydrogen-Ion Concentration ,Platelet Aggregation Inhibitors - Abstract
We studied the platelet aggregation inhibiting activity and ADP degrading activity of human placental villi (tissue culture supernatant) and brush border membrane vesicles (BBMV) and obtained the following results. 1. There existed a platelet aggregation inhibiting activity in tissue culture supernatant of villi (S-villi) but not in that of decidua or amnion. The S-villi inhibited the platelet aggregation induced by ADP, but not that induced by collagen, arachidonic acid or ristocetin. And, there was also ADP degrading activity (ADPase activity) in the S-villi. ADP was quickly degraded by S-villi. When ADP was preincubated with S-villi, the platelet aggregation induced by ADP was completely lost. 2. There was very strong platelet aggregation inhibiting activity in placental BBMV. The BBMV almost completely inhibited the platelet aggregation induced by ADP, collagen, arachidonic acid and ristocetin. And there was very strong ADP degrading activity in the placental BBMV. ADP was quickly degraded by BBMV. When ADP was preincubated with BBMV, the platelet aggregation induced by ADP was completely lost. 3. The enzymatic character (heat stability, enzymatic kinetics, Ca++ dependency and pH dependency) of ADP degrading activity in BBMV was very similar to that in S-villi. 4. The ADP degrading activity of both S-villi and solubilized BBMV were fractionated by anion exchange column chromatography and gel filtration column chromatography in similar patterns, and it was shown that ADP degrading substance of both S-villi and solubilized BBMV had a molecular weight of about 60K. more...
- Published
- 1992
4. [The study on the degradation of prostacyclin activity in tissue culture supernatant of human placental villi]
- Author
-
H, Iioka, S, Akada, H, Hisanaga, K, Morimoto, I, Moriyama, and M, Ichijo
- Subjects
Platelet Aggregation ,Pregnancy ,Culture Techniques ,Microcirculation ,Placenta ,Humans ,Female ,Chorionic Villi ,Chorionic Gonadotropin ,Epoprostenol ,Platelet Aggregation Inhibitors - Abstract
To clarify the role of placental villi in maintaining microcirculation in the placenta, we investigated the action to protect the activity of prostacyclin in the tissue culture supernatant of human placental villi. 1. The platelet aggregation inhibiting activity of prostacyclin was decreased very rapidly in Ringer buffer. 2. The tissue culture supernatant of human placental villi has an action which protects the activity of prostacyclin. On the other hand, in the tissue culture supernatant of human placental decidua no such action is prominent. 3. Human chorionic gonadotropin (hCG) also has an action which protects the activity of prostacyclin. These results indicated that human placental villi play an important role in maintaining placental microcirculation by producing substances which have a protective on the activity of prostacyclin. more...
- Published
- 1991
5. [A study of underlying immunoglobulin production by neonates]
- Author
-
Y, Kato, S, Saito, M, Maruyama, I, Moriyama, M, Ichijo, and M, Saito
- Subjects
Adult ,Immunoglobulin M ,Neutrophils ,Immunoglobulin G ,Interleukins ,T-Lymphocytes ,Antibody Formation ,Infant, Newborn ,Humans ,Fetal Death ,Infant, Premature - Abstract
During the prenatal period the ability of the fetus mononuclear cells (MNC) to produce antibodies is impaired. 1. In term infants, IgG production with only Staphylococcus aureus Cowan I strain (SAC) stimulation was significantly decreased (p less than 0.00005) as compared with adults. However, when adult MNC supernatant stimulated with Pokeweed mitogen (PWM-sup) was given to full term infants, antibody production significantly increased. 2. The activity inducing antibody production in the full term infant MNC is present primarily in the adult T cell culture supernatant. 3. B cell differentiation factor gamma (BCDF gamma) and BCDF mu, which induce IgG and IgM production, respectively, were significantly reduced in premature and full term infants (p less than 0.00005) as compared with adult. 4. By giving adult PWM-sup to the under 33-week-old infants MNC with SAC stimulation, the induction of both IgG and IgM was reduced, and IgG production was especially inadequate. From the above, it is thought that in full term infants, the production of BCDF gamma and BCDF mu which are produced by T cells is reduced compared to that of adults, and that this is mainly responsible for the reduced antibody production. In addition, in the under 33-week-old infants, the immaturity of B cells and inadequate BCDF production induce underlying poor or inadequate immunoglobulin production. more...
- Published
- 1991
6. [The role of vitamin E during pregnancy--anti-platelet aggregation activity of alpha-tocopherol]
- Author
-
S, Akada, H, Iioka, I, Moriyama, H, Hisanaga, K, Morimoto, and M, Ichijo
- Subjects
Adenosine Diphosphate ,Platelet Aggregation ,Pregnancy ,Humans ,Vitamin E ,Female ,Chorionic Villi ,In Vitro Techniques ,Platelet Activating Factor ,Platelet Aggregation Inhibitors - Abstract
To clarify the role of vitamin E (alpha-tocopherol) during pregnancy, we mainly investigated the relation between platelet function and vitamin E and obtained the following results. 1. The concentration of vitamin E (alpha-tocopherol) in plasma and platelet increased gradually during pregnancy. The concentration of vitamin E in plasma was 6.65 micrograms/ml in non pregnant women and 15.5 micrograms/ml in full term pregnant women. And the concentration of vitamin E in platelet was 99 micrograms/g protein in non pregnant women and 244 micrograms/g protein in full term pregnant women. 2. Vitamin E (alpha-tocopherol) inhibited platelet aggregation induced by ADP (adenosine diphosphate) and PAF(platelet activating factor). At a concentration of 500 micrograms/ml vitamin E almost completely inhibited platelet aggregation induced by ADP and PAF. 3. There exists a strong platelet aggregation inhibiting activity in human placental brush border membrane vesicles. At a protein concentration of 20 micrograms/ml the brush border membrane vesicles almost completely inhibited platelet aggregation induced by ADP and PAF. 4. With the co-existence of the brush border membrane vesicles, the platelet aggregation inhibiting activity of vitamin E (alpha-tocopherol) increased prominently. These results indicate that vitamin E plays an important role in the regulation of platelet function and might contribute to the maintenance of placental microcirculation by inhibiting platelet aggregation. more...
- Published
- 1991
7. [The maintenance of placental circulation--the study on the platelet inhibition activity of placental chorioepithelial brush border membrane]
- Author
-
H, Iioka, H, Hisanaga, I, Moriyama, S, Akada, K, Morimoto, and M, Ichijo
- Subjects
Adenosine Diphosphate ,Thromboxane A2 ,Microvilli ,Ristocetin ,Pregnancy ,Placenta ,Apyrase ,Humans ,Female ,Arachidonic Acids ,Platelet Aggregation Inhibitors ,Trophoblasts - Abstract
We studied the platelet aggregation inhibiting activity of human placental syncytiotrophoblast brush border membrane vesicles (BBMV) and obtained the following results. 1. There existed a very strong platelet aggregation inhibiting activity in placental BBMV. The BBMV almost completely inhibited the platelet aggregation induced by ADP, arachidonic acid and ristocetin. 2. There existed a very strong ADP degrading activity (ADPase activity) in the placental BBMV. ADP was quickly decomposed to AMP by BBMV. 3. Placental BBMV inhibited platelet TXA2 (TXB2) production. In the 0.04 mg/ml protein concentration of BBMV, platelet TXA2 production was almost completely inhibited. 4. There existed a very strong platelet aggregation inhibiting activity in solved BBMV. Soluble BBMV almost completely inhibited the platelet aggregation induced by ADP and arachidonic acid but did not inhibit the platelet aggregation induced by ristocetin. 5. ADP decomposing activity and platelet TXA2 production inhibiting activity were separated by anion exchange column chromatography of soluble BBMV. 6. When BBMV was treated with ultrasonic waves to destroy the membrane structure, only the activity inhibiting ristocetin-induced platelet aggregation was markedly decreased. more...
- Published
- 1990
8. [D-glucose reabsorption activity of fetal kidney (by using brush border membrane vesicles of proximal tubule)]
- Author
-
H, Iioka, I, Moriyama, K, Itoh, K, Hino, Y, Okamura, Y, Itani, Y, Kato, T, Ibaragi, and M, Ichijo
- Subjects
Kidney Tubules, Proximal ,Glucose ,Fetal Organ Maturity ,Microvilli ,Sodium ,Humans ,Biological Transport ,Gestational Age ,In Vitro Techniques ,Absorption ,Membrane Potentials - Abstract
By using brush border membrane vesicles prepared from human mid gestational fetal kidney (cortex), the D-glucose transport mechanism and its developmental pattern was studied by the rapid filtration technique. 1. The transport of D-glucose into brush border membrane vesicles prepared from fetal kidney (18 gestational weeks) did not show a great degree of Na+ gradient (extravesicular greater than intravesicular) dependence. 2. The transport of D-glucose into brush border membrane vesicles prepared from fetal kidney (22 gestational weeks) depended on Na+ gradient. This Na+ dependent D-glucose transport showed overshoot and markedly increased when the intravesicular space was rendered electrically more negative with a membrane potential induced by highly permeant anions or by the K+ diffusion membrane potential via valinomycin. These results indicated that the Na+ dependent active D-glucose reabsorption system in the proximal tubules of kidney might be formed between 18 and 22 gestational weeks and the D-glucose reabsorption mechanism of kidney at 22 gestational weeks was Na+ cotransport dependent on membrane potential as in adult ones. more...
- Published
- 1987
9. [The study on human placental DHA-S transport mechanism (using placental microvillous membrane vesicles)]
- Author
-
H, Iioka, I, Moriyama, M, Akazaki, M, Oku, K, Itoh, K, Hino, Y, Okamura, Y, Itani, Y, Katoh, and M, Ichijo
- Subjects
Dose-Response Relationship, Drug ,Microvilli ,Dehydroepiandrosterone Sulfate ,Pregnancy ,Placenta ,Temperature ,Humans ,Biological Transport ,Female ,Dehydroepiandrosterone - Abstract
To investigate the placental DHA-S (dehydroepiandrosterone sulfate) transport mechanism, the uptake of DHA-S into microvillous membrane vesicles prepared from human term placenta was studied using the rapid filtration technique. 1. The uptake of DHA-S into microvillous membrane vesicles was not dependent on both Na+ electrochemical gradient and membrane potential difference. 2. The uptake of DHA-S into microvillous membrane vesicles was dependent on temperature. The initial uptake rate of DHA-S at 37 degrees C was three times as great as at 4 degrees C. 3. The initial rate of DHA-S transport exhibited saturation kinetics with respect to the DHA-S concentration; an apparent Km of 0.067 mM and Vmax of 1.01 nmol/mg protein/20 sec were calculated. 4. The uptake of DHA-S into microvillous membrane vesicles was inhibited by DHA, but not by estriol. These results indicated that placental DHA-S transport was carrier mediated and a passive one. more...
- Published
- 1987
10. [Immunoglobulin production of neonate--T lymphocyte subset and production of cytokine]
- Author
-
M, Saito, S, Saito, I, Moriyama, T, Ibaragi, and M, Ichijo
- Subjects
Biological Products ,Interleukins ,T-Lymphocytes ,Infant, Newborn ,Immunoglobulins ,Fetal Blood ,Pregnancy ,Cytokines ,Humans ,Interleukin-2 ,Female ,Interleukin-4 ,Infant, Premature ,Interleukin-1 - Abstract
On the mechanism underlying the repression of antibody production encountered in the new-born, in this study we used the induction of antibody-producing cells on mitogen stimulation of PWM and SAC, identification of T-cell subsets through two-color FACS and the evaluation of cytokine production of IL-1, IL-2 and BCGF with the following results; 1. The cord blood IgG level rose with gestational weeks due to active transport from the mother. The IgM level was low in full term infants. A natural antibody first appeared in the 16th week. 2. Antibody production on mitogen stimulation in infants was remarkably less than in adults. The inability to produce antibodies appears to be closely related to T-cell factors. 3. In examination of the cell surface maker, new-born infants show less suppressor T-cell but significantly more suppressor inducer T-cell than in adults and that its much smaller number of helper T-cells are insufficient for antibody production. 4. Production of IL-1 in full term infants was significantly lower and that in premature subjects was much lower than that in adults. In full term infants, IL-2 was about the same as in adults, and BCGF production was greater than in adults not depressed in full term infants, and were significantly higher in premature infants than that in adults. more...
- Published
- 1987
11. The study of placental L-ascorbate (vitamin C) transport mechanism (using microvillous membrane vesicles)
- Author
-
H, Iioka, I, Moriyama, M, Kyuma, M, Akasaki, Y, Katoh, K, Itoh, K, Hino, Y, Okamura, Y, Ninomiya, and Y, Kiyozuka
- Subjects
Osmotic Pressure ,Pregnancy ,Culture Techniques ,Humans ,Biological Transport ,Female ,Ascorbic Acid ,Chorionic Villi - Abstract
To investigate the placental L-ascorbate (Vitamin C) transport mechanism, the uptake of L-ascorbate into microvillous membrane vesicles prepared from human term placenta was studied using the rapid filtration technique. The uptake of L-ascorbate into microvillous membrane vesicles was osmotically sensitive. This finding indicated that the uptake of L-ascorbate into microvillous membrane vesicles represented transport into the vesicles. The uptake of L-ascorbate into microvillous membrane vesicles was not dependent on sodium electrochemical gradient. The initial rate of uptake was not changed when the intravesicular space was rendered electrically more negative by membrane diffusion potential induced by the use of highly permeant anions. The initial rate of L-ascorbate transport exhibited saturation kinetics with respect to the L-ascorbate concentration; an apparent Km of 1.33 mM and Vmax of 47p mol/mg protein/20 sec was calculated. The uptake of L-ascorbate into microvillous membrane vesicles was competitively inhibited by D-isoascorbate. These results indicated that transport of L-ascorbate across the placental microvillous membrane vesicles was carrier mediated and was passive transport. more...
- Published
- 1987
12. [Nephritogenic glycopeptide (nephritogenoside): its existence in the urine of toxemia patients]
- Author
-
H, Iioka, I, Moriyama, M, Amasaki, K, Itoh, and M, Ichijo
- Subjects
Electrophoresis ,Receptors, Concanavalin A ,Pre-Eclampsia ,Pregnancy ,Placenta ,Humans ,Enzyme-Linked Immunosorbent Assay ,Female ,Glycoproteins - Abstract
Previously we reported that we succeeded in isolating from human full term placenta, glycopeptide which was immunologically the same as nephritogenic glycopeptide (Nephritogenoside) prepared from human renal GBM (glomerular basement membrane). This time to clarify the correlation between the pathology of toxemia and Nephritogenoside, we examined the existence of Nephritogenoside in the urine of toxemia patients. To purify Nephritogenoside from the urine powder of toxemia patients, we use the same procedures (enzyme digestion, Con A affinity column chromatography e.t.c.) as used in purifying Nephritogenoside from human full term placental TrBM (trophoblast basement membrane). In the urine of toxemia patients we clarified the existence of glycopeptide, which had specific affinity with Con A and was immunologically the same as Nephritogenoside. The existence of Nephritogenoside in the urine of toxemia patients further strengthened the possibility of a relationship between Nephritogenoside and the pathology of toxemia. more...
- Published
- 1985
13. [The platelet aggregation inhibition activity of placental brush border membrane]
- Author
-
H, Hisanaga, H, Iioka, I, Moriyama, M, Akasaki, K, Nabuchi, Y, Katagami, and M, Ichijo
- Subjects
Microvilli ,Platelet Aggregation ,Pregnancy ,Humans ,Female ,Chorionic Villi ,Platelet Aggregation Inhibitors - Published
- 1989
14. [Human placental glutathione transport mechanism]
- Author
-
H, Iioka, I, Moriyama, M, Kyuma, M, Akasaki, Y, Katoh, K, Itoh, M, Saitoh, K, Hino, Y, Okamura, and Y, Itani
- Subjects
Microvilli ,Osmotic Pressure ,Pregnancy ,Placenta ,Sodium ,Biological Transport, Active ,Humans ,Female ,Isoxazoles ,gamma-Glutamyltransferase ,In Vitro Techniques ,Glutathione ,Membrane Potentials - Abstract
The placental transport mechanism of glutathione (GSH) was investigated using microvillous membrane vesicles prepared from human term placenta. Using (3H-glycine)-labeled-GSH, it was clarified that GSH in the extravesicular compartment of placental microvillous membranes was rapidly degraded by gamma-GTP (gamma-glutamyltranspeptidase) and resulting amino acid, and 3H-labeled-glycine was actively transported via a sodium cotransport system. AT-125 treated microvillous membrane vesicles almost entirely lost its gamma-GTP activity, and showed intact GSH transport. Using AT-125 treated microvillous membrane vesicles, it was revealed that GSH was transported across the microvillous membrane as an anion via a membrane potential-dependent mechanism. These results indicated that gamma-GTP which existed in microvillous membrane played a role in GSH metabolism and that intracellular GSH was translocated out of the syncythiotrophoblast cell into the maternal blood space via a specific carrier in microvillous membrane because the GSH concentration was higher in intracellular than extracellular and extracellular membrane potential was positively charged. more...
- Published
- 1987
15. [A study on the mechanism of bile acid transport in the human placenta (the passive transport system of taurocholate across microvillous membrane)]
- Author
-
H, Iioka, I, Moriyama, K, Hino, and M, Ichijo
- Subjects
Bile Acids and Salts ,Taurocholic Acid ,Pregnancy ,Osmolar Concentration ,Temperature ,Humans ,Biological Transport ,Female ,Chorionic Villi ,Maternal-Fetal Exchange ,Membrane Potentials - Abstract
The uptake of taurocholate into microvillous membrane vesicles prepared from human normal full term placenta was studied using a rapid filtration technique. The taurocholate uptake into microvillous membrane vesicles was sensitive to extravesicular osmolarity, and preincubation with the taurocholate increased the uptake of taurocholate into the vesicles. These findings indicate that the uptake of taurocholate by microvillous membrane vesicles represents transport into vesicles. The uptake of taurocholate into vesicles was not dependent on sodium electrochemical gradient (extravesicular greater than intravesicular). But, this uptake was markedly increased when the intravesicular space was rendered electrically more positive by the use of lowly permeant anions or K+ diffusion potentials via valinomycin. These findings indicated that taurocholate was transported into microvillous membrane vesicles as anion. Cholic acid inhibited the uptake of taurocholate into vesicles, but taurine didn't inhibit this uptake. The initial rate of taurocholate transport exhibited saturation kinetics with respect to the taurocholate concentration; an apparent Km of 67 microM and Vmax of 0.30n mol/mg protein/20 sec were calculated. These results indicated that placental taurocholate transport was not active but passive (facilitated diffusion), and taurocholate was transported from fetus to mother via placenta because the blood concentration was higher in fetus than mother. more...
- Published
- 1986
16. [A study on the constitutional change of taurine and other free amino acids during the fetal development (author's transl)]
- Author
-
I, Moriyama, S, Ueda, M, Akasaki, and M, Ichijo
- Subjects
Taurine ,Pregnancy Trimester, Third ,Postpartum Period ,Infant, Newborn ,Growth ,Amniotic Fluid ,Fetal Blood ,Rats ,Fetus ,Pregnancy ,Animals ,Humans ,Female ,Amino Acids - Abstract
In human pregnancy, maternal and umbilical blood of the 22nd week of pregnancy and of the parturition were submitted to the analysis of total free amino acids (FAA) by amino acids analyzer. In addition, the neonatal blood was also submitted until 9 days after birth. The content of FAA in umbilical vein was higher than that of the maternal blood and it decreased at the parturition. However, the content of taurine (T) kept extremely higher level than other amino acids until term and it decreased to the normal level at 9 days after birth. In pregnant rats on day 17, 18 and 19 of gestation, the content of FAA in umbilical vein and fetal artery were 5800-7000 micrograms/dl and 5700-6250 micrograms/dl, respectively. They were two fold higher than that of maternal value. In amniotic fluid, FAA was 4100 micrograms/dl on day 18 and 8850 micrograms/dl on day 20. On the contrary, the content of T in maternal blood on day 18 was the highest value (360 micrograms /dl), and decreased to the half of the value on day 20. T in carotid artery on the same day was 1150 micrograms/dl, but it decreased to 280 micrograms/dl on day 20. In amniotic fluid, it was almost constant in the range of 380-400 micrograms/dl. When the pregnant rat was stressed by starvation, FAA was increased in both umbilical and fetal blood but it was almost constant in amniotic fluid. T was increased both in maternal and fetal blood. When the degree of starvation, T value also increased in fetal blood. From these data, we discussed on the possible mechanism of the production of T during pregnancy and after birth. more...
- Published
- 1982
17. [The effect of inorganic mercury on placental amino acid transport using microvillous membrane vesicles]
- Author
-
H, Iioka, I, Moriyama, M, Oku, K, Hino, Y, Itani, Y, Okamura, and M, Ichijo
- Subjects
Alanine ,Microvilli ,Pregnancy ,Lysine ,Micropore Filters ,Placenta ,Humans ,Biological Transport ,Female ,Mercury ,Amino Acids - Abstract
To study the toxic effect of inorganic mercury on the placenta we elucidate the effect of inorganic mercury on placental amino acid transport using microvillous membrane vesicles isolated from human normal full term placenta. The transport of amino acids into microvillous membrane vesicles was studied by a rapid filtration technique using a millipore filter. The transport of L-alanine across placental microvillous membrane was Na+ electrochemical gradient dependent and 0.1 mM inorganic mercury inhibited 77% of this Na+ dependent L-alanine transport and 1 mM inorganic mercury inhibits 90% of this Na+ dependent L-alanine transport. The transport of L-lysine across microvillous membrane vesicles was sodium independent and 0.1 mM inorganic mercury inhibited 34% of this transport and 1 mM inorganic mercury inhibited 50% of this transport. These results indicated that one of the toxic effects of inorganic mercury on placenta-fetus unit was the inhibition of placental nutrient transport. more...
- Published
- 1987
18. [The effect of antibiotics (gentamicin) on placental amino acid transport activity (using human placental microvillous membrane vesicles)]
- Author
-
H, Iioka, I, Moriyama, K, Itoh, K, Hino, Y, Okamura, Y, Itani, Y, Katoh, T, Shimamoto, and M, Ichijo
- Subjects
Alanine ,Microvilli ,Placenta ,Biological Transport, Active ,Humans ,Amino Acids ,Chorionic Villi ,Gentamicins - Abstract
In order to elucidate the effect of antibiotics (gentamicin) on placental amino acids transport, we investigated L-alanine transport using microvillous membrane vesicles prepared from full-term human placental by a rapid filtration technique. 1. The active transport of L-alanine into microvillous membrane vesicles was dependent on Na+ electrochemical gradient (extravesicular greater than intravesicular). And the double reciprocal plot of this Na+ dependent initial uptake rate versus L-alanine concentration exhibited an apparent Km of 0.79 + 0.23mM and a Vmax of 3.56 + 0.70n mol/mg protein/20 sec. 2. Gentamicin did not affect the Km value of this Na+ dependent L-alanine transport kinetics (0.77 + 0.19 mM [lmM gentamicin], 0.79 + 0.21mM [10mM gentamicin]). On the other hand, gentamicin apparently decreased, the Vmax value of this transport kinetics (1.99 + 0.48n mol/mg protein/20 sec [1mM gentamicin], 1.12 + 0.32n mol/mg protein/20 sec [10mM gentamicin]). more...
- Published
- 1987
19. [Nephritogenic glycoprotein isolated from human placenta--purification and isolation by sonication]
- Author
-
H, Iioka, I, Moriyama, K, Itoh, M, Amasaki, M, Ichijo, and S, Shibata
- Subjects
Electrophoresis ,Sonication ,Pregnancy ,Placenta ,Kidney Glomerulus ,Chromatography, Gel ,Concanavalin A ,Humans ,Female ,Amino Acids ,Basement Membrane ,Chromatography, Affinity ,Glycoproteins - Abstract
Previously, we reported that we succeeded in isolating from human placenta a substance comparable to human renal nephritogenic glycoprotein prepared from glomerular basement membrane (GBM). This time we purified a sample isolated from human placental trophoblast basement membrane (TrBM) with the methods for purifying renal nephritogenic glycoprotein and clarified its chemical composition. To purify the sample prepared from human placental TrBM, we fractionated the sample by Zone Electrophoresis, Concanavalin A(Con A) affinity column chromatography and Bio Gel P200 column chromatography. As the active fraction of the sample purified from human placental TrBM bound specifically with Con A, it proved to be a glycoprotein. The monosaccharide composition of this glycoprotein was rich in glucose (glucose, galactose and mannose were in the ratio of 1.00:0.30:0.33) and the amino acid composition of this glycoprotein contained no collagenous components. Ouchterlony gel diffusion demonstrated the existence of a common precipitin line between antiserum to human renal nephritogenic glycoprotein prepared from GBM and human renal nephritogenic glycoprotein prepared from GBM, purified human placental glycoprotein, the sample prepared from human placental TrBM by sonication and nephritogenic glycoprotein prepared from urine of S.L.E. patient. more...
- Published
- 1984
20. [The changes in EPH gestosis placental amino acid transport activity (using human placental microvillous membrane vesicles)]
- Author
-
H, Iioka, I, Moriyama, M, Akazaki, K, Itoh, K, Hino, Y, Okamura, Y, Itani, Y, Katoh, and M, Ichijo
- Subjects
Alanine ,Microvilli ,Pre-Eclampsia ,Pregnancy ,Placenta ,Biological Transport, Active ,Humans ,Female ,Amino Acids ,Alkaline Phosphatase - Abstract
To elucidate the change in EPH gestosis placental amino acid transport activity, we investigated the uptake of L-alanine into microvillous membrane vesicles prepared from EPH gestosis placenta and from normal placenta by using a rapid filtration technique. 1. Alkaline phosphatase (ALP) was the marker enzyme of microvillous membrane vesicles (MMV). The ALP activity of mild EPH gestosis placental MMV didn't differ from that of normal placental MMV. On the other hand, the ALP activity of severe EPH gestosis placental MMV decreased compared to that of normal placental MMV. 2. The uptake of L-alanine into human placental MMV was dependent on the Na+ electrochemical gradient, so the transport across human placental MMV was a secondarily active one. The L-alanine transport activity of mild EPH gestosis placental MMV didn't differ from that of normal placental MMV. On the other hand, the L-alanine transport activity of severe EPH gestosis placental MMV decreased prominently compared to that of normal placental MMV. more...
- Published
- 1987
21. [Effects of food additive hydrogen peroxide studied in fetal development]
- Author
-
I, Moriyama, K, Hiraoka, M, Fujita, M, Ichijo, H, Ioka, and S, Kanoh
- Subjects
Fetal Diseases ,Fetus ,Pregnancy ,Animals ,Birth Weight ,Female ,Food Additives ,Hemorrhage ,Rats, Inbred Strains ,Growth ,Hydrogen Peroxide ,Rats - Published
- 1982
22. [Study on the mechanism of placental transport of L-lysine (using human placental microvillous (brush border) membrane vesicles)]
- Author
-
H, Iioka, I, Moriyama, M, Kyuma, M, Amasaki, K, Itoh, and M, Ichijo
- Subjects
Ornithine ,Cell Membrane Permeability ,Microvilli ,Glutamine ,Lysine ,Placenta ,Biological Transport ,Arginine ,Membrane Potentials ,Pregnancy ,Humans ,Female ,Cilia ,Chorionic Villi - Abstract
The uptake of L-lysine in human placental microvilli vesicles prepared from human term placenta was studied using the rapid filtration technique. The uptake of L-lysine into the vesicles was osmotically sensitive. This finding indicates that the uptake of L-lysine by placental microvilli vesicles represents transport into the membrane vesicles. The uptake of L-lysine into microvilli vesicles is sodium independent. The initial rate of uptake is markedly increased when the intravesicular space is rendered electrically more negative by membrane diffusion potential induced by the use of highly permeant anions or by K+ diffusion potentials via valinomycin. These results indicate that the sodium independent uptake of L-lysine into the microvilli membrane vesicles is dependent on the electrical potential difference of the membranes. A kinetic analysis of the uptake demonstrated that two transport systems for vesicular entry of L-lysine existed with a Km1 of 0.13 mM, Vmax1 of 590 pmol/mg protein/20 sec, Km2 of 0.91 mM, Vmax2 of 2010 pmol/mg protein/20 sec. The uptake of L-lysine in microvilli vesicles was inhibited by dibasic amino acid (L-arginine, L-ornithine, L-glutamine), but not by other classes of amino acid. These results indicate the existence of a dibasic amino acid specific transport system in placental microvilli membrane. more...
- Published
- 1985
23. [Absorption of L-alanine in small intestine of human fetus (by using brush border membrane vesicles of jejunum)]
- Author
-
H, Iioka, I, Moriyama, K, Itoh, K, Hino, Y, Okamura, Y, Itani, Y, Kato, T, Ibaragi, and M, Ichijo
- Subjects
Kinetics ,Alanine ,Jejunum ,Fetal Organ Maturity ,Intestinal Absorption ,Microvilli ,Sodium ,Potassium ,Humans ,In Vitro Techniques ,Membrane Potentials - Abstract
By using brush border membrane vesicles prepared from human mid gestational fetal intestine (jejunum), the intestinal L-alanine transport mechanism was studied using a rapid filtration technique. The uptake of L-alanine by the vesicles was osmotically sensitive. This finding indicated that the uptake of L-alanine by the vesicles represented transport into the vesicles. A Na+ electrochemical gradient (extravesicular greater than intravesicular) stimulated the initial rate of L-alanine uptake, and Na+ dependent uptake of L-alanine into vesicles showed a typical overshoot phenomenon. This overshoot and the initial rate of uptake markedly increased when the intravesicular space was rendered electrically more negative by membrane diffusion potentials induced by with highly permeant anions. A similar stimulation of L-alanine uptake was observed, when membrane potential (inside negative) was imposed by K+ diffusion potentials via valinomycin. These results indicated that an Na+ dependent uptake of L-alanine into the brush border membrane vesicles was dependent on the electrical potential difference of the membrane. The initial rate of L-alanine transport exhibited saturation kinetics with respect to the L-alanine concentration; an apparent Km of Km1; 1.6mM, Km2; 14.1mM, and Vmax of Vmax1; 4.0n mol/mg protein/20sec, Vmax2; 8.2n mol/mg protein/20 sec were calculated. From these results mid gestational fetal intestine (jejunum) already has an L-alanine absorption system which is comparable to the adult one's. more...
- Published
- 1987
24. [The study on the human placental L-lactate transport mechanism (using placental microvillous membrane vesicles)]
- Author
-
K, Nabuchi, I, Moriyama, M, Akasaki, Y, Katakami, H, Hisanaga, Y, Kato, and M, Ichijo
- Subjects
Microvilli ,Pregnancy ,Placenta ,Sodium ,Lactates ,Humans ,Female ,In Vitro Techniques ,Maternal-Fetal Exchange ,Hydrogen - Abstract
To elucidate the human placental L-lactate transport mechanism, we investigated L-lactate uptake by microvillous membrane vesicles (MMV) prepared from full termed human placenta using the rapid filtration technique. 1. The transport of L-lactate into the MMV was not dependent on the Na+ electrochemical gradient (extravesicular greater than intravesicular). 2. The transport of L-lactate into the MMV was dependent on the H+ gradient (extravesicular greater than intravesicular) and this H+ dependent L-lactate uptake showed a tendency to overshoot. This overshoot disappeared with the addition of H+ ionophore (FCCP). 3. The initial rate of this H+ dependent L-lactate transport into the MMV exhibited saturation kinetics with respect to the L-lactate concentration: An apparent Km of 4.35mM and Vmax of 2.78n mol/mg protein/20sec were calculated. These results indicated that there existed a H+ and L-lactate transport system in human placental MMV. more...
- Published
- 1989
25. [Studies on the mechanism of placental transport of L-glutamate (the effect of K+ in microvillous vesicles on L-glutamate uptake)]
- Author
-
H, Iioka, I, Moriyama, K, Itoh, K, Hino, and M, Ichijo
- Subjects
Ions ,Cell Membrane Permeability ,Dose-Response Relationship, Drug ,Glutamates ,Microvilli ,Pregnancy ,Placenta ,Sodium ,Potassium ,Humans ,Female ,In Vitro Techniques ,Membrane Potentials - Abstract
The effect of potassium ion and membrane potential on the uptake of L-glutamate in microvilli (brush border) vesicles prepared from human term placenta was studied using a rapid filtration technique. The uptake of L-glutamate into microvilli vesicles is Na+ electrochemical gradient dependent and pre-equilibration of the vesicles with K+ stimulates L-glutamic acid uptake. Imposition of a K+ gradient (K+ in greater than K+ out) enhances Na+-dependent L-glutamate uptake. Changes in membrane potential due to the imposition of anion replacement markedly affect Na+ dependent L-glutamate uptake only in the presence of K+. However, this effect is not significant when changes in membrane potential incur following the imposition of valinomycin induced by K+ diffusion potential. The data indicate that Na+-dependent L-glutamate transport can be additionally energized by a K+ gradient. Furthermore K+ renders Na+-dependent L-glutamate transport sensitive to changes in the transmembrane potential difference. more...
- Published
- 1985
26. [Study on placental L-glutamine transport mechanism using microvilli vesicles]
- Author
-
H, Iioka, I, Moriyama, K, Hino, and M, Ichijo
- Subjects
Kinetics ,Microvilli ,Osmotic Pressure ,Pregnancy ,Glutamine ,Placenta ,Humans ,Biological Transport ,Female ,Amino Acids ,In Vitro Techniques ,Maternal-Fetal Exchange ,Membrane Potentials - Abstract
Using microvillous (brush border) membrane vesicles prepared from human term placenta, the uptake of L-glutamine was studied using a rapid filtration technique. The uptake of L-glutamine into the vesicles was osmotically sensitive. A Na+ electrochemical gradient (extravesicular greater than intravesicular) stimulated the initial rate of L-glutamine uptake and the Na+ dependent uptake of L-glutamine into vesicles showed a typical overshoot phenomenon. This overshoot and the initial rate of uptake were markedly increased when the intravesicular space was rendered electrically more negative by membrane diffusion potentials, induced by the use of highly permeant anions. A similar stimulation of L-glutamine uptake was observed when membrane potential (inside negative) was imposed by K+ diffusion potentials via valinomycin. These results indicated that a sodium dependent uptake of L-glutamine into the microvillous membrane vesicles was dependent on the electrical potential difference of membrane. The initial rate of L-glutamine transport exhibited saturation kinetics with respect to L-glutamine concentration; the apparent Km of 0.42 mM and Vmax of 1.54 nmol/mg protein/20 sec were calculated. The uptake of L-glutamine into the vesicles was competitively inhibited by L-alanine. more...
- Published
- 1986
27. [The character of human placental glucose and amino acid transport activity (using microvillous membrane vesicles)]
- Author
-
H, Iioka, I, Moriyama, Y, Katoh, K, Itoh, K, Hino, Y, Okamura, Y, Itani, and M, Ichijo
- Subjects
Alanine ,Glucose ,Microvilli ,Pregnancy ,Placenta ,Humans ,Biological Transport ,Female ,Amino Acids - Abstract
To elucidate the character of human placental D-glucose and L-alanine transport activity, we investigated the uptake of D-glucose and L-alanine into microvillous membrane vesicles prepared from human early and term placenta using the rapid filtration technique. 1. The uptake of D-glucose into microvillous membrane vesicles did not depend on the Na+ gradient (extravesicular greater than intravesicular). The uptake of D-glucose into vesicles was three times as great as that of L-glucose. And phloretin prominently inhibited the uptake of D-glucose into vesicles. So, it was indicated that the transport mechanism of D-glucose across microvillous membrane was facilitated diffusion. 2. The uptake of L-alanine into microvillous membrane vesicles depended on Na+ gradient (extravesicular greater than intravesicular), so that the transport mechanism of L-alanine across microvillous membrane was a secondary active one. The transport of L-alanine into vesicles prepared from term placenta increased prominently compared to that of early placenta. On the other hand, the transport activity of D-glucose into vesicles prepared from term placenta did not differ from that of early placenta. more...
- Published
- 1987
28. [Placental transport of amino acid]
- Author
-
M, Ichijo, I, Moriyama, and H, Iioka
- Subjects
Ions ,Microvilli ,Placenta ,Sodium ,Biological Transport ,In Vitro Techniques ,Membrane Potentials ,Trophoblasts ,Glutamates ,Osmotic Pressure ,Pregnancy ,Potassium ,Humans ,Female ,Amino Acids ,Chlorine - Published
- 1985
29. [Ontogenic development of the human neutrophil]
- Author
-
M, Saito, S, Saito, I, Moriyama, K, Hino, and M, Ichijo
- Subjects
Phagocytosis ,Neutrophils ,Infant, Newborn ,Humans ,Cell Differentiation ,Infant, Premature - Published
- 1987
30. [Study on the mechanism of placental transport of phosphate (using human placental microvillous (brush border) membrane vesicles)]
- Author
-
H, Iioka, I, Moriyama, M, Amasaki, K, Itoh, K, Hino, and M, Ichijo
- Subjects
Anions ,Cell Membrane Permeability ,Valinomycin ,Microvilli ,Placenta ,Osmolar Concentration ,Sodium ,Biological Transport ,Hydrogen-Ion Concentration ,In Vitro Techniques ,Phosphates ,Pregnancy ,Humans ,Female - Abstract
Using the rapid filtration technique, the uptake of phosphate into microvillous (brush border) membrane vesicles isolated from human placental trophoblast was investigated. The microvillous membrane vesicles exhibit the uptake of phosphate into an osmotically reactive intravesicular space and preincubation with the phosphate increased the uptake of phosphate into the vesicles. These findings indicate that the uptake of phosphate by placental trophoblast microvillous membranes represents transport into membrane vesicles. In the presence of sodium electrochemical gradient (extravesicular greater than intravesicular) the uptake of phosphate by vesicles shows an overshoot phenomenon. Sodium-dependent phosphate uptake is about two times higher at pH 6.0 as in the uptake observed at pH 8.0. The initial rate of sodium-dependent phosphate transport exhibited saturation kinetics with respect to phosphate concentration: An apparent Km of 0.28 mM and Vmax of 330 pmol/mg protein in 20 seconds were calculated. These results indicate that the transport of phosphate across the microvillous membranes is carrier mediated. Experiments with different anions (SCN-, Cl-, gluconate-) and ionophore (valinomycin) showed that at pH 7.4 phosphate transport in the presence of a Na+ gradient is almost independent of electrical potential across the vesicle membranes. It was concluded that placental trophoblast microvillous membranes contain an electroneutral Na+/phosphate co-transport system. more...
- Published
- 1985
31. [Study on human placental beta-alanine and taurine transport mechanism (using microvillous membrane vesicles]
- Author
-
H, Iioka, I, Moriyama, M, Akasaki, K, Itoh, K, Hino, Y, Kato, Y, Okamura, Y, Itani, and M, Ichijo
- Subjects
Ions ,Alanine ,Microvilli ,Pregnancy ,Taurine ,Placenta ,Sodium ,beta-Alanine ,Humans ,Biological Transport ,Female - Abstract
Using microvillous membrane vesicles prepared from human full term placenta, we studied the placental beta-amino acid transport mechanism. The transport of amino acids into microvillous membrane vesicles was studied by a filtration technique using a millipore filter. The uptake of beta-alanine into microvillous membrane vesicles was dependent on Na+ electrochemical gradient (extravesicular greater than intravesicular). The initial rate of this Na+ gradient dependent beta-alanine transport exhibited saturation kinetics with respect to the beta-alanine concentration: an apparent Km of 0.24 mM and Vmax of 46 pmol/mg protein/20 sec were calculated. Taurine inhibited beta-alanine uptake into microvillous membrane vesicles, but on the other hand L-alanine didn't inhibit this beta-alanine uptake. The L-alanine uptake into microvillous membrane vesicles was Na+ electrochemical gradient dependent and the initial rate of this Na+ dependent L-alanine uptake into vesicles was faster than the uptake of Na+ itself into vesicles. On the other hand, the initial rate of Na+ dependent beta-alanine and taurine uptake into vesicles was slower than the uptake of Na+ itself into vesicles. These results indicated that there existed a beta-amino acid specific transport system in human placental microvillous membrane, and placental taurine transport was carried out by this system. And it was also indicated that this placental beta-amino acid transport mechanism is quite different from that of L-alanine. more...
- Published
- 1987
32. [The study on the platelet aggregation inhibition activity of placental brush border (the mechanism of platelet aggregation inhibition)]
- Author
-
H, Iioka, H, Hisanaga, I, Moriyama, M, Akasaki, K, Nabuchi, Y, Katakami, Y, Katoh, and M, Ichijo
- Subjects
Blood Platelets ,Microvilli ,Platelet Aggregation ,Placenta ,Apyrase ,Arachidonic Acids ,Adenosine Diphosphate ,Ristocetin ,Pregnancy ,Malondialdehyde ,Humans ,Female ,Collagen ,Platelet Aggregation Inhibitors - Abstract
To clarify the role of placental brush border in the regulation of placental microcirculation, we investigated the platelet aggregation inhibition activity of placental brush border membrane vesicles (BBMV) and obtained the following results. 1. There was very strong platelet aggregation inhibiting activity in placental BBMV. In the 20-40 micrograms/ml protein concentration, the BBMV almost completely inhibited the platelet aggregation induced by ADP, arachidonic acid and collagen, and in the 100-150 micrograms/ml protein concentration, the BBMV almost completely inhibited the platelet aggregation induced by ristocetin. 2. There existed a very strong ADP degrading activity (ADPase activity) in the placental BBMV. The platelet aggregation activity of ADP was completely lost when ADP was preincubated with the placental BBMV. 3. The placental BBMV inhibited platelet malondialdehyde (MDA) production. With the 20 micrograms/ml and 100 micrograms/ml protein concentrations of placental BBMV, 22% and 65% of platelet MDA production was inhibited respectively. more...
- Published
- 1989
33. [Nephritogenic glycoprotein isolated from human placenta (methods of isolation from placenta]
- Author
-
H, Iioka, I, Moriyama, M, Ichijo, and S, Shibata
- Subjects
Electrophoresis ,Placenta ,Chromatography, Gel ,Humans ,Enzyme-Linked Immunosorbent Assay ,Female ,Chorionic Villi ,Pregnancy Proteins ,Basement Membrane ,Glycoproteins - Abstract
In our experiments, we succeeded in obtaining from human placenta a substance comparable to human renal nephritogenic glycoprotein prepared from GBM (glomerular basement membrane). The procedure was quite similar to that for preparation from the kidney. At first, placental terminal villi were isolated by the successive use of three metal sieves. Then trophoblast basement membranes (TrBM) were isolated from placental terminal villi by ultrasonic disruption. Finally TrBM were made soluble by trypsin digestion, after which they were further digested by pronase. Ouchterlony gel diffusion demonstrated the existence of a common precipitin line between antiserum to human renal nephritogenic glycoprotein prepared from GBM and antiserum to the sample prepared from human placental TrBM, and human renal nephritogenic glycoprotein prepared from GBM and the sample prepared from human placental TrBM. The monosaccharide composition of the sample prepared from human placental TrBM was rich in glucose (glucose, galactose and mannoside in the ratio of 1.00 : 0.74 : 1.00), and the amino acid composition of the sample contained no collagenous components. As a result of fractionation of the sample prepared from human placental TrBM by Bio Gel P200 column chromatography, the activity of renal nephritogenic glycoprotein was found within the void fraction. more...
- Published
- 1983
34. [Studies on L-glutamate transport mechanism in human placental trophoblast microvilli membrane vesicles]
- Author
-
H, Iioka, I, Moriyama, M, Kyuma, K, Ito, M, Amasaki, and M, Ichijo
- Subjects
Ions ,Organoids ,Aspartic Acid ,Glutamates ,Microvilli ,Pregnancy ,Sodium ,Humans ,Biological Transport ,Female ,Maternal-Fetal Exchange ,Cells, Cultured ,Trophoblasts - Abstract
The uptake of L-glutamate in brush border (microvilli) vesicles prepared from human term placenta was studied using the rapid filtration technique. The uptake of L-glutamate into the vesicles occurred osmotically, and preincubation with L-glutamate increased the uptake of amino acid. These findings indicate that the uptake of L-glutamate by placental trophoblast brush border membranes represents the transport into membrane vesicles. A Na+ electrochemical gradient (extravesicular greater than intravesicular) stimulated the initial rate of L-glutamate uptake about three times. The initial rate of transport exhibited saturation kinetics with respect to the L-glutamate concentration; an apparent Km of 0.15 mM and V max of 70 pmol/mg protein in 20 seconds were calculated. The uptake of L-glutamate into the vesicles was competitively inhibited by L-glutamate and L-cysteate (acidic amino acid). These results indicate that a Na-dependent acidic amino acid specific transport system exists in the placental trophoblast microvilli membrane. These results indicate that the transport of L-glutamate across the placental microvilli membrane is sodium-dependent and carrier mediated. more...
- Published
- 1985
35. [The transport mechanism of antibiotics using microvillous membrane vesicles (placental transport of fosfomycin)]
- Author
-
H, Iioka, I, Moriyama, M, Kyuma, Y, Tsuji, and M, Ichijo
- Subjects
Cell Membrane Permeability ,Fosfomycin ,Microvilli ,Pregnancy ,Placenta ,Osmolar Concentration ,Humans ,Biological Transport ,Female ,In Vitro Techniques ,Trophoblasts - Abstract
Using the rapid filtration technique, the uptake of fosfomycin into microvillous membrane vesicles isolated from human term placental trophoblast was investigated. The microvillous membrane vesicles exhibited the uptake of fosfomycin into an osmotically reactive intravesicular space and it was indicated that the uptake of fosfomycin by microvillous membrane vesicles represented transport into membrane vesicles. The uptake of fosfomycin by microvillous membrane vesicles was not dependent on the Na+ electrochemical gradient or membrane potential. The initial uptake of fosfomycin by microvillous membrane vesicles did not exhibit saturation kinetics with respect to fosfomycin concentration, and increased linearly as the fosfomycin concentration increased. These results indicated that fosfomycin was transported across the microvillous membrane by simple diffusion. L-alanine, L-valine, L-lysine, inorganic phosphate or D-glucose did not inhibit the uptake of fosfomycin into microvillous membrane vesicles. On the other hand, fosfomycin did not inhibit the uptake of L-alanine, L-valine, L-lysine inorganic phosphate or D-glucose into microvillous membrane vesicles. These results revealed that fosfomycin did not affect the placental transport activity of other nutrients. more...
- Published
- 1986
36. [Nonenzymatic glucosylation of human placental trophoblast basement membrane collagen (relation to diabetic placenta pathology)]
- Author
-
H, Iioka, I, Moriyama, M, Kyuma, M, Saitoh, M, Oku, K, Hino, Y, Okamura, Y, Itani, and M, Ichijo
- Subjects
Adult ,Glucose ,Glycosylation ,Pregnancy ,Placenta ,Pregnancy in Diabetics ,Humans ,Female ,Collagen ,In Vitro Techniques ,Basement Membrane ,Trophoblasts - Abstract
Nonenzymatic glucosylation is a reaction in which glucose binds nonenzymatically to hemoglobin, serum protein and glomerular basement membrane collagen etc. It has been thought that nonenzymatic glucosylation results in functional and chemical changes in those substances (hemoglobin etc) and contributes to the pathological changes in diabetes mellitus. This time we investigated whether nonenzymatic glucosylation occurred in human placental trophoblast basement (TrBM) collagen or not. The ability of glucose to interact with TrBM collagen (nonenzymatic glucosylation of TrBM collagen) was examined by incubating TrBM collagen with 3H-D-glucose in vitro. As a result it was shown that nonenzymatic glucosylation occurred in TrBM collagen and nonenzymatic glucosylation of TrBM collagen depended on the glucose concentration, reaction time and reaction temperature. These results indicate that possibly hyperglycemia, via nonenzymatic glucosylation modifies the function and chemistry of TrBM collagen and is related to the placental pathological changes in diabetic pregnancy. more...
- Published
- 1987
37. [The role of glutathione on placental amino acid transport (using microvillous membrane vesicles)]
- Author
-
H, Iioka, I, Moriyama, K, Itoh, K, Hino, Y, Okamura, Y, Itani, Y, Katoh, and M, Ichijo
- Subjects
Microvilli ,Pregnancy ,Lysine ,Placenta ,Humans ,Biological Transport ,Female ,Mercury ,Amino Acids ,Glutathione - Abstract
To elucidate the role of glutathione (GSH) on placental amino acid transport, we investigated L-lysine transport using microvillous membrane vesicles prepared from full term human placenta. 1. The transport of L-lysine into microvillous membrane vesicles was not affected by glutathione. 2. The transport of L-lysine into microvillous membrane vesicles was inhibited by inorganic mercury (Hg2+), and 0.1mM Hg2+ inhibited 34% of this transport and 1mM Hg2+ inhibited 50%. 3. The transport of L-lysine inhibited by Hg2+ was almost completely restored when glutathione was added simultaneously. These results indicated that glutathione defended the inhibitory action of inorganic mercury on L-lysine transport across microvillous membrane. more...
- Published
- 1987
38. [Ontogenic development of human natural killer (NK) cells and lymphokine activated killer (LAK) cells]
- Author
-
S, Saito, M, Saito, I, Moriyama, K, Hino, and M, Ichijo
- Subjects
Adult ,Killer Cells, Natural ,Male ,Fetus ,Infant, Newborn ,Humans ,Interleukin-2 ,Female ,Gestational Age ,Flow Cytometry ,Infant, Premature - Abstract
Ontogenic development of human NK cells and LAK cells was evaluated in fetuses in spontaneous abortion, premature infants and full term infants. Additionally, we examined the effect of the production of interleukin-2(IL-2) of cord blood lymphocytes on PHA stimulation. The results were as follows. The NK activity of full term infants was significantly lower than that of adults. But augmented NK activity by recombinant IL-2 (20 U/ml) of term infants was the same as adult levels. However, before 32 weeks gestation, both NK activity and augmented NK activity by recombinant IL-2 were lower than that in full term infants. Using two color flow cytometry, after 16 weeks gestation, the percentage of Leu7- Leu11+ cells was almost equal to the adult level, but only a small number of Leu7+ Leu11+ and Leu7+ Leu11- lymphocytes was noted in premature infants and full term infants. LAK activity was evaluated in fetuses. Fetus that had advanced to 19 weeks of gestation had sufficient LAK activity. After 16 weeks gestation, fetal lymphocytes had good IL-2 production. Therefore in infants, LAK cell can play an important role in immunological surveillance against neoplastic tumor. more...
- Published
- 1987
39. [The character of premature baby's blood taurine concentration and urinary taurine excretion]
- Author
-
H, Iioka, I, Moriyama, Y, Katoh, K, Itoh, K, Hino, Y, Itani, and M, Ichijo
- Subjects
Taurine ,Infant, Newborn ,Humans ,Gestational Age ,Amino Acids ,Infant, Premature - Abstract
The concentration of taurine in fetal blood and in mother's milk is very high. In the present study, in order to elucidate the nutritional role of taurine during fetal and neonatal age we investigated the urinary taurine excretion and the blood taurine concentration change in premature babies. 1. The blood taurine concentration of infants on the first postnatal day decreased along with gestation between 7-10 gestational months. On the other hand, there was no difference in the concentrations of blood total amino acids in infants on the first postnatal day in 7-10 gestational months. 2. The blood taurine concentration of infants decreased remarkably during one week after birth. This tendency to decrease was more prominent in premature infants--those at 8 to 10 gestational months. 3. The urinary excretion of taurine and total amino acids of infants remarkably decreased along with gestation between 7-10 gestational months. This tendency to decrease was the most prominent in infants between 7 and 8 gestational months. more...
- Published
- 1988
40. [Relationship of fetal hind limb and fetal liver on amino acid metabolism in pregnant rats]
- Author
-
M, Kyuma, Y, Tsuji, H, Iioka, I, Moriyama, and M, Ichijo
- Subjects
Male ,Embryonic and Fetal Development ,Glutamates ,Liver ,Pregnancy ,Animals ,Pregnancy, Animal ,Extremities ,Female ,Rats, Inbred Strains ,Amino Acids ,Hindlimb ,Rats - Abstract
A tracer technique for injections into rat fetal abdomen in utero was employed to obtain a better knowledge of amino acid metabolism in fetal hind limb and fetal liver. Calculation of tissue fluid distribution via 3H-inulin space made possible an estimation of the fetal hind limb and fetal liver intracellular amino acid concentration based upon the fetal plasma and fetal tissue amino acid concentration. A significant concentration gradient between fetal plasma and fetal hind limb was found for glutamate (37.3) but not for alanine (9.7) or leucine (3.0). The radioactivity of fetal hind limb and fetal liver after the injection of 1 microCi of radioactive 14C-glutamate 14C-alanine or 14C-leucine into fetal abdomen was measured. In fetal hind limb, significant radioactivity was recognized after 14C-alanine administration, but not after 14C-glutamate administration as against the significant concentration gradient. However, in fetal liver, significant radioactivity was recognized after 14C-glutamate administration, but not after 14C-alanine administration. We concluded that in fetus, glutamate was released from the hind limbs and a large amount taken up by fetal liver. more...
- Published
- 1987
41. [Study on Na+ and L-alanine cotransport of the human placenta using microvillous membrane vesicles]
- Author
-
H, Iioka, I, Moriyama, K, Itoh, M, Saito, K, Hino, Y, Okamura, Y, Itani, K, Kakimoto, T, Ibaragi, and M, Ichijo
- Subjects
Ions ,Alanine ,Microvilli ,Pregnancy ,Placenta ,Sodium ,Humans ,Biological Transport ,Female ,In Vitro Techniques ,Membrane Potentials - Abstract
Using microvillous membrane vesicles prepared from human normal term placenta, placental cotransport system of Na+ and L-alanine was studied using rapid filtration technique. The uptake of L-alanine into microvillous membrane vesicles was Na+ ion electrochemical gradient (extravesicular greater than intravesicular) dependent and showed typical overshoot phenomenon. Both Na+ dependent L-alanine uptake and Na+ ion uptake into microvillous membrane were membrane potential dependent and were markedly increased when the intravesicular space was rendered electrically more negative by membrane diffusion potentials, induced by the use of highly permeant anions. L-alanine gradient (extravesicular greater than intravesicular) induced a temporary accumulation of Na+ ions. These results indicated that L-alanine and Na+ ions were cotransported across microvillous membrane and this cotransport was dependent on the electrical potential difference in the membrane. more...
- Published
- 1987
42. [Study on changes in placental L-alanine transport activity during gestation (using microvillous membrane vesicles]
- Author
-
H, Iioka, I, Moriyama, M, Saito, K, Hino, Y, Okamura, and M, Ichijo
- Subjects
Pregnancy Trimester, First ,Alanine ,Microvilli ,Pregnancy ,Placenta ,Pregnancy Trimester, Third ,Humans ,Female ,Intracellular Membranes - Abstract
Using microvillous membrane vesicles prepared from human normal early and full term placenta, we investigated the transport mechanism of L-alanine and the change in its transport activity during gestation. We estimated the purity of microvillous membrane vesicles prepared from human placenta from the relative specific activities (microvilli versus homogenate) of the membrane's maker enzymes, alkaline phosphatase (ALP), 5'-nucleotidase, and gamma-glutamyltranspeptidase ( gamma-GTP). In early pregnancy (12-15 weeks gestational age), the relative specific activities (microvilli versus homogenate) were calculated to be: ALP: 15.3, 5'-nucleotidase: 14.0, gamma-GTP: 8.3, and in full term pregnancy (37-40 weeks gestational age) the relative specific activities (microvilli versus homogenate) were calculated to be: ALP: 16.0, 5'-nucleotidase: 14.8, gamma-GTP: 7.5. The uptake of L-alanine into microvillous membrane vesicles was Na+ electrochemical gradient (extravesicular greater than intravesicular) dependent and this Na+ dependent uptake was membrane-potentially sensitive both in early pregnancy and in full term pregnancy. The kinetics parameter of the initial L-alanine uptake into microvillous membrane vesicles were calculated to be: Km: 0.78 +/- 0.20 mM, Vmax: 0.62 +/- 0.21 nmol/mg protein/20 sec in early pregnancy, Km: 0.80 +/- 0.24 mM, Vmax: 3.53 +/- 0.70 nmol/mg protein/20 sec in full term pregnancy. In conclusion, the placental transport mechanisms of L-alanine in both early and full term pregnancy were the same, and the L-alanine transport activity of full term pregnancy was much greater than that of early pregnancy. more...
- Published
- 1986
43. [Action of steroid hormone in control of metabolism and its significance in initiation of pregnancy and gestation]
- Author
-
T, Sugawa, I, Moriyama, C, Tamori, S, Matsumoto, and K, Moriguchi
- Subjects
Embryonic and Fetal Development ,Pregnancy ,Reproduction ,Uterus ,Animals ,RNA ,Estrogens ,Female ,DNA ,Growth ,Progesterone ,Rats - Published
- 1970
44. [Cerebral and hepatic vasospasm in a case of HELLP syndrome complicated with eclampsia].
- Author
-
Sakamoto Y, Saito S, Moriyama I, Iioka H, Ibaraki T, Akada S, Shimamoto T, Yamada Y, Yoshida T, and Ichijo M
- Subjects
- Adult, Female, Humans, Pregnancy, Eclampsia complications, HELLP Syndrome complications, Ischemia complications, Ischemic Attack, Transient complications, Liver Circulation
- Published
- 1995
45. [Glucose transport in human placental chorioepithelium].
- Author
-
Yamada Y, Moriyama I, Iioka H, Akada S, Hashimoto H, Shimamoto T, Sakamoto Y, and Ichijou M
- Subjects
- Biological Transport, Epithelium metabolism, Female, Humans, In Vitro Techniques, Monosaccharide Transport Proteins metabolism, Pregnancy, Chorion metabolism, Glucose metabolism, Placenta metabolism
- Published
- 1993
46. [The significance of determination of human herpesvirus-6 (HHV-6) antibody during pregnancy].
- Author
-
Kakimoto K, Ando Y, Moriyama I, and Ichijo M
- Subjects
- Abortion, Spontaneous etiology, Adult, Female, Fluorescent Antibody Technique, Herpesviridae Infections diagnosis, Humans, Pregnancy, Pregnancy Complications, Infectious diagnosis, Antibodies, Viral blood, Herpesvirus 6, Human immunology, Immunoglobulin G blood, Immunoglobulin M blood
- Abstract
HHV-6 which was detected in peripheral blood lymphocytes of lymphocytic patients is now said to be an agent causing exanthema subitum. We investigated the titer of HHV-6 antibody in 100 primigravida and 30 patients with spontaneous abortion by means of the indirect immunofluorescence method (IF method). The positive incidence in which the titer of HHV-6 antibody was more than 10 times in 100 primigravida was 82%. That of HHV-6 IgM antibody was 3% in those who had no complication during pregnancy. This supposes that there may be some women who were infected or were reactivated while they were pregnant. The positive incidence in 30 spontaneous abortion patients was 90%. 3 of them had IgM antibody to HHV-6, and HHV-6 antigen was detected on the epitherium of the chorionic villi by the IF method with HHV-6 monoclonal antibody in two of them. This suggests that the infection or reactivation of HHV-6 may be one of the causes of spontaneous abortions. more...
- Published
- 1992
47. [The platelet aggregation inhibiting activity in human placenta--study on the ADP degrading activity of human placental villi].
- Author
-
Iioka H, Hisanaga H, Akada S, Moriyama I, and Ichijo M
- Subjects
- Apyrase isolation & purification, Calcium, Female, Hot Temperature, Humans, Hydrogen-Ion Concentration, Platelet Aggregation Inhibitors isolation & purification, Apyrase metabolism, Chorionic Villi metabolism, Platelet Aggregation Inhibitors metabolism
- Abstract
We studied the platelet aggregation inhibiting activity and ADP degrading activity of human placental villi (tissue culture supernatant) and brush border membrane vesicles (BBMV) and obtained the following results. 1. There existed a platelet aggregation inhibiting activity in tissue culture supernatant of villi (S-villi) but not in that of decidua or amnion. The S-villi inhibited the platelet aggregation induced by ADP, but not that induced by collagen, arachidonic acid or ristocetin. And, there was also ADP degrading activity (ADPase activity) in the S-villi. ADP was quickly degraded by S-villi. When ADP was preincubated with S-villi, the platelet aggregation induced by ADP was completely lost. 2. There was very strong platelet aggregation inhibiting activity in placental BBMV. The BBMV almost completely inhibited the platelet aggregation induced by ADP, collagen, arachidonic acid and ristocetin. And there was very strong ADP degrading activity in the placental BBMV. ADP was quickly degraded by BBMV. When ADP was preincubated with BBMV, the platelet aggregation induced by ADP was completely lost. 3. The enzymatic character (heat stability, enzymatic kinetics, Ca++ dependency and pH dependency) of ADP degrading activity in BBMV was very similar to that in S-villi. 4. The ADP degrading activity of both S-villi and solubilized BBMV were fractionated by anion exchange column chromatography and gel filtration column chromatography in similar patterns, and it was shown that ADP degrading substance of both S-villi and solubilized BBMV had a molecular weight of about 60K. more...
- Published
- 1992
48. [The study on the degradation of prostacyclin activity in tissue culture supernatant of human placental villi].
- Author
-
Iioka H, Akada S, Hisanaga H, Morimoto K, Moriyama I, and Ichijo M
- Subjects
- Chorionic Gonadotropin pharmacology, Chorionic Villi physiology, Culture Techniques, Epoprostenol pharmacology, Female, Humans, Microcirculation, Placenta blood supply, Platelet Aggregation drug effects, Platelet Aggregation Inhibitors pharmacology, Pregnancy, Chorionic Villi metabolism, Epoprostenol metabolism
- Abstract
To clarify the role of placental villi in maintaining microcirculation in the placenta, we investigated the action to protect the activity of prostacyclin in the tissue culture supernatant of human placental villi. 1. The platelet aggregation inhibiting activity of prostacyclin was decreased very rapidly in Ringer buffer. 2. The tissue culture supernatant of human placental villi has an action which protects the activity of prostacyclin. On the other hand, in the tissue culture supernatant of human placental decidua no such action is prominent. 3. Human chorionic gonadotropin (hCG) also has an action which protects the activity of prostacyclin. These results indicated that human placental villi play an important role in maintaining placental microcirculation by producing substances which have a protective on the activity of prostacyclin. more...
- Published
- 1991
49. [The role of vitamin E during pregnancy--anti-platelet aggregation activity of alpha-tocopherol].
- Author
-
Akada S, Iioka H, Moriyama I, Hisanaga H, Morimoto K, and Ichijo M
- Subjects
- Adenosine Diphosphate antagonists & inhibitors, Chorionic Villi blood supply, Female, Humans, In Vitro Techniques, Platelet Activating Factor antagonists & inhibitors, Platelet Aggregation Inhibitors pharmacology, Vitamin E blood, Platelet Aggregation drug effects, Pregnancy blood, Vitamin E physiology
- Abstract
To clarify the role of vitamin E (alpha-tocopherol) during pregnancy, we mainly investigated the relation between platelet function and vitamin E and obtained the following results. 1. The concentration of vitamin E (alpha-tocopherol) in plasma and platelet increased gradually during pregnancy. The concentration of vitamin E in plasma was 6.65 micrograms/ml in non pregnant women and 15.5 micrograms/ml in full term pregnant women. And the concentration of vitamin E in platelet was 99 micrograms/g protein in non pregnant women and 244 micrograms/g protein in full term pregnant women. 2. Vitamin E (alpha-tocopherol) inhibited platelet aggregation induced by ADP (adenosine diphosphate) and PAF(platelet activating factor). At a concentration of 500 micrograms/ml vitamin E almost completely inhibited platelet aggregation induced by ADP and PAF. 3. There exists a strong platelet aggregation inhibiting activity in human placental brush border membrane vesicles. At a protein concentration of 20 micrograms/ml the brush border membrane vesicles almost completely inhibited platelet aggregation induced by ADP and PAF. 4. With the co-existence of the brush border membrane vesicles, the platelet aggregation inhibiting activity of vitamin E (alpha-tocopherol) increased prominently. These results indicate that vitamin E plays an important role in the regulation of platelet function and might contribute to the maintenance of placental microcirculation by inhibiting platelet aggregation. more...
- Published
- 1991
50. [A study of underlying immunoglobulin production by neonates].
- Author
-
Kato Y, Saito S, Maruyama M, Moriyama I, Ichijo M, and Saito M
- Subjects
- Adult, Fetal Death immunology, Humans, Immunoglobulin G biosynthesis, Immunoglobulin M biosynthesis, Infant, Premature immunology, Interleukins physiology, Neutrophils immunology, T-Lymphocytes immunology, Antibody Formation, Infant, Newborn immunology
- Abstract
During the prenatal period the ability of the fetus mononuclear cells (MNC) to produce antibodies is impaired. 1. In term infants, IgG production with only Staphylococcus aureus Cowan I strain (SAC) stimulation was significantly decreased (p less than 0.00005) as compared with adults. However, when adult MNC supernatant stimulated with Pokeweed mitogen (PWM-sup) was given to full term infants, antibody production significantly increased. 2. The activity inducing antibody production in the full term infant MNC is present primarily in the adult T cell culture supernatant. 3. B cell differentiation factor gamma (BCDF gamma) and BCDF mu, which induce IgG and IgM production, respectively, were significantly reduced in premature and full term infants (p less than 0.00005) as compared with adult. 4. By giving adult PWM-sup to the under 33-week-old infants MNC with SAC stimulation, the induction of both IgG and IgM was reduced, and IgG production was especially inadequate. From the above, it is thought that in full term infants, the production of BCDF gamma and BCDF mu which are produced by T cells is reduced compared to that of adults, and that this is mainly responsible for the reduced antibody production. In addition, in the under 33-week-old infants, the immaturity of B cells and inadequate BCDF production induce underlying poor or inadequate immunoglobulin production. more...
- Published
- 1991
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.