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Your search keyword '"Lelandais-Brière C"' showing total 2 results
Start Over Author "Lelandais-Brière C" Journal new phytologist
2 results on '"Lelandais-Brière C"'

1. A miR169 isoform regulates specific NF-YA targets and root architecture in Arabidopsis.

Author

Sorin C, Declerck M, Christ A, Blein T, Ma L, Lelandais-Brière C, Njo MF, Beeckman T, Crespi M, and Hartmann C

Subjects
Arabidopsis cytology, Arabidopsis growth & development, Arabidopsis Proteins metabolism, CCAAT-Binding Factor metabolism, Gene Expression, Genes, Reporter, Meristem cytology, Meristem genetics, Meristem growth & development, MicroRNAs metabolism, Phenotype, Plant Roots cytology, Plant Roots genetics, Plant Roots growth & development, Plants, Genetically Modified, RNA Isoforms, RNA, Plant genetics, RNA, Plant metabolism, Arabidopsis genetics, Arabidopsis Proteins genetics, CCAAT-Binding Factor genetics, Gene Expression Regulation, Plant, MicroRNAs genetics
Abstract

In plants, roots are essential for water and nutrient acquisition. MicroRNAs (miRNAs) regulate their target mRNAs by transcript cleavage and/or inhibition of protein translation and are known as major post-transcriptional regulators of various developmental pathways and stress responses. In Arabidopsis thaliana, four isoforms of miR169 are encoded by 14 different genes and target diverse mRNAs, encoding subunits A of the NF-Y transcription factor complex. These miRNA isoforms and their targets have previously been linked to nutrient signalling in plants. By using mimicry constructs against different isoforms of miR169 and miR-resistant versions of NF-YA genes we analysed the role of specific miR169 isoforms in root growth and branching. We identified a regulatory node involving the particular miR169defg isoform and NF-YA2 and NF-YA10 genes that acts in the control of primary root growth. The specific expression of MIM169defg constructs altered specific cell type numbers and dimensions in the root meristem. Preventing miR169defg-regulation of NF-YA2 indirectly affected laterial root initiation. We also showed that the miR169defg isoform affects NF-YA2 transcripts both at mRNA stability and translation levels. We propose that a specific miR169 isoform and the NF-YA2 target control root architecture in Arabidopsis., (© 2014 The Authors. New Phytologist © 2014 New Phytologist Trust.)

Published
2014
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2. Transcriptional and post-transcriptional regulation of a NAC1 transcription factor in Medicago truncatula roots.

Author

D'haeseleer K, Den Herder G, Laffont C, Plet J, Mortier V, Lelandais-Brière C, De Bodt S, De Keyser A, Crespi M, Holsters M, Frugier F, and Goormachtig S

Subjects
Amino Acid Sequence, Arabidopsis genetics, Arabidopsis metabolism, Arabidopsis Proteins genetics, Arabidopsis Proteins metabolism, Base Sequence, Flowers drug effects, Flowers genetics, Flowers physiology, Gene Expression Regulation, Plant, Indoleacetic Acids pharmacology, Medicago truncatula drug effects, Medicago truncatula genetics, MicroRNAs genetics, Molecular Sequence Data, Mutation, Phylogeny, Plant Leaves drug effects, Plant Leaves genetics, Plant Leaves physiology, Plant Proteins chemistry, Plant Proteins genetics, Plant Root Nodulation genetics, Plant Roots drug effects, Plant Roots genetics, Plant Roots physiology, Plant Shoots drug effects, Plant Shoots genetics, Plant Shoots physiology, Plants, Genetically Modified drug effects, Plants, Genetically Modified genetics, Plants, Genetically Modified physiology, Protein Structure, Tertiary, RNA, Plant genetics, Recombinant Fusion Proteins, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae metabolism, Nicotiana genetics, Nicotiana metabolism, Trans-Activators genetics, Trans-Activators metabolism, Transcription Factors chemistry, Transcription Factors genetics, Medicago truncatula physiology, Plant Proteins metabolism, Sinorhizobium meliloti physiology, Transcription Factors metabolism
Abstract

• Legume roots develop two types of lateral organs, lateral roots and nodules. Nodules develop as a result of a symbiotic interaction with rhizobia and provide a niche for the bacteria to fix atmospheric nitrogen for the plant. • The Arabidopsis NAC1 transcription factor is involved in lateral root formation, and is regulated post-transcriptionally by miRNA164 and by SINAT5-dependent ubiquitination. We analyzed in Medicago truncatula the role of the closest NAC1 homolog in lateral root formation and in nodulation. • MtNAC1 shows a different expression pattern in response to auxin than its Arabidopsis homolog and no changes in lateral root number or nodulation were observed in plants affected in MtNAC1 expression. In addition, no interaction was found with SINA E3 ligases, suggesting that post-translational regulation of MtNAC1 does not occur in M. truncatula. Similar to what was found in Arabidopsis, a conserved miR164 target site was retrieved in MtNAC1, which reduced protein accumulation of a GFP-miR164 sensor. Furthermore, miR164 and MtNAC1 show an overlapping expression pattern in symbiotic nodules, and overexpression of this miRNA led to a reduction in nodule number. • This work suggests that regulatory pathways controlling a conserved transcription factor are complex and divergent between M. truncatula and Arabidopsis., (© 2011 The Authors. New Phytologist © 2011 New Phytologist Trust.)

Published
2011
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