1. Quantification of target proteins using hydrogel antibody arrays and MALDI time-of-flight mass spectrometry (A2M2S)
- Author
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Jörg Tost, Andrei Alexandrovich Stomakhin, Nelly Papin, Diane Lebeau, Ekaterina Dementieva, Ekaterina Darii, Sascha Sauer, Alla Rubina, Alexander S. Zasedatelev, E. N. Savvateeva, Ivo Gut, and Alexander A. Makarov
- Subjects
Immunoassay ,Proteomics ,Chromatography ,Resolution (mass spectrometry) ,Protein mass spectrometry ,Chemistry ,Quantitative proteomics ,Protein Array Analysis ,Proteins ,Bioengineering ,Hydrogels ,General Medicine ,Tandem mass tag ,Isotope-coded affinity tag ,Label-free quantification ,Isobaric labeling ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,Time-of-flight mass spectrometry ,Molecular Biology ,Biotechnology - Abstract
Mass spectrometry-based analysis techniques are widely applied in proteomics. This study presents a novel method for quantitative multiplex candidate protein profiling. It applies immunocapture of differentially labeled protein complements on hydrogel antibody arrays and subsequent quantification by MS. To make this approach quantitative a labeling approach was devised. The impact of labeling on the antibody/antigen interaction was assessed in detail by surface plasmon resonance. Owing to the resolution by mass more than two protein samples can be compared simultaneously. Direct labeling of crude samples such as sera was developed and so enables the absolute quantification of target proteins straight from crude samples without a protein purification step. It was used to measure the concentration of apolipoprotein A-1 in serum. This method has been termed A2M2S for Affinity Arrays and MALDI Mass Spectrometry.
- Published
- 2008