1. Alpha-synuclein immunoreactivity patterns in the enteric nervous system.
- Author
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Aldecoa I, Navarro-Otano J, Stefanova N, Sprenger FS, Seppi K, Poewe W, Cuatrecasas M, Valldeoriola F, Gelpi E, and Tolosa E
- Subjects
- Adult, Aged, Aged, 80 and over, Biomarkers metabolism, Case-Control Studies, Colonic Neoplasms metabolism, Colonic Neoplasms surgery, Female, Gastrointestinal Tract innervation, Gastrointestinal Tract metabolism, Gastrointestinal Tract surgery, Humans, Inflammatory Bowel Diseases metabolism, Inflammatory Bowel Diseases surgery, Lewy Bodies metabolism, Male, Middle Aged, Myenteric Plexus metabolism, Parkinson Disease surgery, Protein Aggregates, Young Adult, Enteric Nervous System metabolism, Parkinson Disease metabolism, alpha-Synuclein metabolism
- Abstract
We aimed to compare immunoreactivity patterns of four different anti-α-syn antibodies in surgical specimens of the gastrointestinal tract of Parkinson disease and control cases. Surgical specimens from stomach, small and large bowel of 6 PD cases and 12 controls were studied. Primary antibodies: anti-α-syn clone KM51, anti-phosphorylated α-syn Ser129, anti-α-syn clone 15G7 and anti-nitrated α-syn505. We found different immunoreactivity patterns: (a) coarse, Lewy-body-like aggregates labelled by the 4 antibodies and detected in 4/6 PD cases and in 1/12 controls; (b) distinct punctate cytoplasmic staining of ganglion cells labelled by anti-phosphorylated-α-syn and detected in 3/6 PD cases and 3/12 controls; (c) fine diffuse, synaptic-type staining of neural structures labelled by anti-α-syn-15G7 and anti-nitrated-α-syn505 and detected in all subjects. We conclude that different specific and non-specific immunoreactivity patterns are detected in surgical specimens of gastrointestinal tract when using different anti-α-syn antibodies, as they recognize different epitopes and states of alpha-synuclein protein. Coarse aggregates in neural structures seem to be the most promising marker for the diagnosis of Lewy-body parkinsonism when evaluating abnormal α-syn in the gastrointestinal tract., (Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.)
- Published
- 2015
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