1. JAK2/STAT3 Axis Intermediates Microglia/Macrophage Polarization During Cerebral Ischemia/Reperfusion Injury
- Author
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Yi Zhong, Lijuan Gu, Yingze Ye, Hua Zhu, Bei Pu, Jinchen Wang, Yuntao Li, Sheng Qiu, Xiaoxing Xiong, and Zhihong Jian
- Subjects
Inflammation ,Male ,Mice ,Macrophages ,Reperfusion Injury ,General Neuroscience ,Animals ,Infarction, Middle Cerebral Artery ,Microglia ,Brain Ischemia ,Ischemic Stroke - Abstract
Subtypes of microglia/macrophage regulate the inflammation in the opposite direction during ischemic stroke. JAK2/STAT3 signaling pathway participates in the development of stroke-related inflammation via ischemic stimulation. However, the relationship between JAK2/STAT3 pathway and microglia/macrophage phenotype transformation is unclear.This study established a transient middle cerebral artery occlusion (tMCAO) model in male STAT3For the conditioned STAT3-KO mice, the infarction was significantly increased after MCAO, accompanied by the aggravation of neurological deficit. Higher expression of iNOS and CD16/32 than Arg-1, Ym-1, and CD206 in vivo and in vitro, and decreased p-STAT3/STAT3 ratio in STAT3Collectively, these results reveal that JAK2/STAT3 signaling pathway regulates the microglia/macrophage polarization (skewing toward the M2 polarization) during the CIRI, thus alleviating brain damage. Therefore, approaches targeting JAK2/STAT3 activation are promising therapies for ischemic stroke.
- Published
- 2022