Search

Your search keyword '"Harrower T"' showing total 3 results
Start Over Author "Harrower T" Journal neuroreport
3 results on '"Harrower T"'

2. Gal is widely expressed in embryonic porcine stem cells and neural tissue

Author

Harrower, T. P., Richards, A., Cruz, G., Copeman, L., Dunnett, S. B., and Barker, R. A.

Abstract

Fetal porcine neural xenografts are an alternative to human fetal tissue for cell based treatments of a number of neurodegenerative conditions but are currently limited by host immunological rejection. The expression of a major immunological epitope, Gal1-3Gal1-4GlcNAc-R (Gal) was determined on stem cells and primary cells derived from E26 porcine fetal brains. Gal was detected on the majority of neural stem cells and cells from primary cell suspensions. The expression of this epitope paralleled the binding of human IgG and IgM to the cells, a binding that was significantly reduced with anti-Gal depleted human serum. This study demonstrates that Gal expression is extensive in embryonic porcine neural cells and will be of relevance to any clinical trials using this tissue.

Published
2002

3. Alpha Gal is widely expressed in embryonic porcine stem cells and neural tissue.

Author

Harrower TP, Richards A, Cruz G, Copeman L, Dunnett SB, and Barker RA

Subjects
Animals, Brain cytology, Brain embryology, Brain metabolism, Cells, Cultured, Embryo, Mammalian cytology, Endothelium, Vascular cytology, Endothelium, Vascular embryology, Endothelium, Vascular metabolism, Humans, Neurons cytology, Stem Cells cytology, Swine, Embryo, Mammalian metabolism, Epitopes biosynthesis, Neurons metabolism, Stem Cells metabolism, Trisaccharides biosynthesis
Abstract

Fetal porcine neural xenografts are an alternative to human fetal tissue for cell based treatments of a number of neurodegenerative conditions but are currently limited by host immunological rejection. The expression of a major immunological epitope, Galalpha1-3Galbeta1-4GlcNAcbeta-R (alphaGal) was determined on stem cells and primary cells derived from E26 porcine fetal brains. alphaGal was detected on the majority of neural stem cells and cells from primary cell suspensions. The expression of this epitope paralleled the binding of human IgG and IgM to the cells, a binding that was significantly reduced with anti-alphaGal depleted human serum. This study demonstrates that alphaGal expression is extensive in embryonic porcine neural cells and will be of relevance to any clinical trials using this tissue.

Published
2002
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results