1. Guidance and topographic stabilization of nasal chick retinal axons on target-derived components in vitro
- Author
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Ysander von Boxberg, Silvia Deiss, and Uli Schwarz
- Subjects
Electrophoresis ,Superior Colliculi ,Time Factors ,Neurite ,Central nervous system ,Chick Embryo ,Biology ,Chemical Fractionation ,Retina ,chemistry.chemical_compound ,Culture Techniques ,medicine ,Animals ,Membranes ,General Neuroscience ,Embryogenesis ,Histological Techniques ,Retinal ,Axons ,Cell biology ,Nerve Regeneration ,medicine.anatomical_structure ,Membrane protein ,chemistry ,embryonic structures ,Isoelectric Focusing ,Nasal Cavity ,Tectum ,Neuroscience ,Homing (hematopoietic) - Abstract
We studied mechanisms underlying the generation of topographic order within the developing chick retinotectal connection by combining the recently introduced stripe assay with a novel membrane protein fractionation technique. Our experiments show a preference of temporal and nasal retinal fibers for growing on cell membranes prepared from their proper target area. In addition, membrane preparations from posterior tectum were found to prolong substantially the survival of nasal neurites in vitro. We conclude that tropic as well as trophic interactions contribute to the generation of topographic maps during embryogenesis, in our case to the homing of nasal axons within the posterior tectum.
- Published
- 1993