1. An effective inducer of dopaminergic neuron-like differentiation
- Author
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Wenyu, Fu, Cui, Lv, Wenxin, Zhuang, Dandan, Chen, E, Lv, Fengjie, Li, and Xiaocui, Wang
- Subjects
mesenchymal stem cells ,grants-supported paper ,fibroblast growth factor 8 ,differentiation ,all-trans retinoic acid ,sonic hedgehog ,stem cells ,dopaminergic neuron ,catecholamine ,Xiangdan injection ,Neurodegenerative Disease and Neural Regeneration ,neural regeneration ,induction ,photographs-containing paper ,neuroregeneration - Abstract
Rat bone marrow-derived mesenchymal stem cells were cultured and passaged in vitro. After induction with basic fibroblast growth factor for 24 hours, passage 3 bone marrow-derived mesenchymal stem cells were additionally induced into dopaminergic neurons using three different combinations with basic fibroblast growth factor as follows: 20% Xiangdan injection; all-trans retinoic acid + glial-derived neurotrophic factor; or sonic hedgehog + fibroblast growth factor 8. Results suggest that the bone marrow-derived mesenchymal stem cells showed typical neuronal morphological characteristics after induction. In particular, after treatment with sonic hedgehog + fibroblast growth factor 8, the expressions of nestin, neuron-specific enolase, microtubuleassociated protein 2, tyrosine hydroxylase and vesicular monoamine transporter-2 in cells were significantly increased. Moreover, the levels of catecholamines in the culture supernatant were significantly increased. These findings indicate that Xiangdan injection, all-trans retinoic acid + glial-derived neurotrophic factor, and sonic hedgehog + fibroblast growth factor 8 can all induce dopaminergic neuronal differentiation from bone marrow-derived mesenchymal stem cells. In particular, the efficiency of sonic hedgehog + fibroblast growth factor 8 was highest.
- Published
- 2012