1. Proteomic Analysis of Cytokeratin Isoforms Uncovers Association with Survival in Lung Adenocarcinoma
- Author
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Sharon L.R. Kardia, Tarek G. Gharib, David E. Misek, John Yee, Dafydd G. Thomas, Mark B. Orringer, Hong Wang, David G. Beer, Michael S. Prescott, Thomas J. Giordano, Kerby Shedden, Samir M. Hanash, Jeremy M. G. Taylor, Chiang Ching Huang, and Guoan Chen
- Subjects
Male ,Gene isoform ,Cancer Research ,Lung Neoplasms ,Proteome ,Blotting, Western ,Adenocarcinoma ,Biology ,lcsh:RC254-282 ,2D PAGE ,Immunoenzyme Techniques ,Cytokeratin ,Keratin ,Biomarkers, Tumor ,medicine ,Humans ,Protein Isoforms ,Electrophoresis, Gel, Two-Dimensional ,RNA, Messenger ,RNA, Neoplasm ,Intermediate filament ,microarrays ,Survival rate ,Aged ,Oligonucleotide Array Sequence Analysis ,mass spectrometry ,chemistry.chemical_classification ,Gene Expression Profiling ,isoorms ,Prognosis ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,lung adenocarcinoma ,medicine.disease ,Molecular biology ,Gene Expression Regulation, Neoplastic ,Survival Rate ,Gene expression profiling ,Blot ,chemistry ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,Keratins ,Female ,Research Article - Abstract
Cytokeratins. (CK) are intermediate filaments whose expression is often altered in epithelial cancer. Systematic identification of lung adenocarcinoma proteins using two-dimensional polyacrylamide gel electrophoresis and mass spectrometry has uncovered numerous CK isoforms. In this study, 93 lung adenocarcinomas. (64 stage I and 29 stage III) and 10 uninvolved lung samples were quantitatively examined for protein expression. Fourteen of 21 isoforms of CK 7, 8, 18, 19 occurred at significantly higher levels. (P
- Published
- 2002
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