1. One-step optogenetics with multifunctional flexible polymer fibers
- Author
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Park, Seongjun, Guo, Yuanyuan, Jia, Xiaoting, Choe, Han Kyoung, Grena, Benjamin, Kang, Jeewoo, Park, Jiyeon, Lu, Chi, Canales, Andres, Chen, Ritchie, Yim, Yeong Shin, Choi, Gloria B, Fink, Yoel, and Anikeeva, Polina
- Subjects
Biotechnology ,Neurosciences ,Animals ,Basolateral Nuclear Complex ,Brain ,Calcium-Calmodulin-Dependent Protein Kinase Type 2 ,Channelrhodopsins ,Electrodes ,Implanted ,Evoked Potentials ,Hippocampus ,Male ,Mice ,Mice ,Transgenic ,Motor Activity ,Neural Pathways ,Neurons ,Opsins ,Optical Fibers ,Optogenetics ,Photic Stimulation ,Polymers ,Prefrontal Cortex ,Psychology ,Cognitive Sciences ,Neurology & Neurosurgery - Abstract
Optogenetic interrogation of neural pathways relies on delivery of light-sensitive opsins into tissue and subsequent optical illumination and electrical recording from the regions of interest. Despite the recent development of multifunctional neural probes, integration of these modalities in a single biocompatible platform remains a challenge. We developed a device composed of an optical waveguide, six electrodes and two microfluidic channels produced via fiber drawing. Our probes facilitated injections of viral vectors carrying opsin genes while providing collocated neural recording and optical stimulation. The miniature (
- Published
- 2017