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Your search keyword '"Vierstra, Jeff"' showing total 4 results
Start Over Author "Vierstra, Jeff" Journal nature methods
4 results on '"Vierstra, Jeff"'

2. Functional footprinting of regulatory DNA

Author

Vierstra, Jeff, primary, Reik, Andreas, additional, Chang, Kai-Hsin, additional, Stehling-Sun, Sandra, additional, Zhou, Yuanyue, additional, Hinkley, Sarah J, additional, Paschon, David E, additional, Zhang, Lei, additional, Psatha, Nikoletta, additional, Bendana, Yuri R, additional, O'Neil, Colleen M, additional, Song, Alexander H, additional, Mich, Andrea K, additional, Liu, Pei-Qi, additional, Lee, Gary, additional, Bauer, Daniel E, additional, Holmes, Michael C, additional, Orkin, Stuart H, additional, Papayannopoulou, Thalia, additional, Stamatoyannopoulos, George, additional, Rebar, Edward J, additional, Gregory, Philip D, additional, Urnov, Fyodor D, additional, and Stamatoyannopoulos, John A, additional

Published
2015
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4. Coupling transcription factor occupancy to nucleosome architecture with DNase-FLASH.

Author

Vierstra, Jeff, Wang, Hao, John, Sam, Sandstrom, Richard, and Stamatoyannopoulos, John A

Subjects
*TRANSCRIPTION factors, *CHROMATIN, *GENOMICS, *DEOXYRIBONUCLEASES, *GENETIC regulation, *COUPLING reactions (Chemistry)
Abstract

It is currently not possible to resolve the genome-wide relationship of transcription factors (TFs) and nucleosomes at the level of individual chromatin templates despite rapidly increasing data on TF and nucleosome occupancy in the human genome. Here we describe DNase I-released fragment-length analysis of hypersensitivity (DNase-FLASH), an approach that directly couples mapping of TF occupancy, via quantification of DNA microfragments released from individual TF recognition sites in regulatory DNA, to the surrounding nucleosome architecture, via analysis of larger DNA fragments, in a single assay. DNase-FLASH enables coupling of individual TF footprints to nucleosome occupancy, identifying TFs that precisely demarcate the regulatory DNA-nucleosome interface. [ABSTRACT FROM AUTHOR]

Published
2014
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