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Your search keyword '"Michael R, Stoneman"' showing total 3 results
Start Over Author "Michael R, Stoneman" Journal nature methods
3 results on '"Michael R, Stoneman"'

1. A general method to quantify ligand-driven oligomerization from fluorescence-based images

Author

Graeme Milligan, Michael R. Stoneman, Richard J. Ward, Ionel Popa, Valerica Raicu, John D. Pediani, Annie Eis, Dammar N. Badu, and Gabriel Biener

Subjects
Agonist, 0303 health sciences, General method, biology, medicine.drug_class, Ligand, Cell Biology, Mass spectrometry, Biochemistry, Fluorescence, 03 medical and health sciences, chemistry.chemical_compound, Monomer, chemistry, Biophysics, medicine, biology.protein, Secretin receptor, Epidermal growth factor receptor, Molecular Biology, 030304 developmental biology, Biotechnology
Abstract

Here, we introduce fluorescence intensity fluctuation spectrometry for determining the identity, abundance and stability of protein oligomers. This approach was tested on monomers and oligomers of known sizes and was used to uncover the oligomeric states of the epidermal growth factor receptor and the secretin receptor in the presence and absence of their agonist ligands. This method is fast and is scalable for high-throughput screening of drugs targeting protein–protein interactions.

Published
2019
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3. A general method to quantify ligand-driven oligomerization from fluorescence-based images

Author

Michael R, Stoneman, Gabriel, Biener, Richard J, Ward, John D, Pediani, Dammar, Badu, Annie, Eis, Ionel, Popa, Graeme, Milligan, and Valerică, Raicu

Subjects
ErbB Receptors, Microscopy, Confocal, Spectrometry, Fluorescence, Image Processing, Computer-Assisted, Humans, Protein Interaction Domains and Motifs, Protein Multimerization, Ligands, Fluorescence, Protein Binding, Receptors, G-Protein-Coupled, Receptors, Gastrointestinal Hormone, Signal Transduction
Abstract

Here, we introduce fluorescence intensity fluctuation spectrometry for determining the identity, abundance and stability of protein oligomers. This approach was tested on monomers and oligomers of known sizes and was used to uncover the oligomeric states of the epidermal growth factor receptor and the secretin receptor in the presence and absence of their agonist ligands. This method is fast and is scalable for high-throughput screening of drugs targeting protein-protein interactions.

Published
2018

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