1. High-throughput epitope discovery reveals frequent recognition of neo-antigens by [CD4.sup.+] T cells in human melanoma
- Author
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Linnemann, Carsten, van Buuren, Marit M., Bies, Laura, Verdegaal, Els M.E., Schotte, Remko, Calis, Jorg J.A., Behjati, Sam, Velds, Arno, Hilkmann, Henk, el Atmioui, Dris, Visser, Marten, Stratton, Michael R., Haanen, John B.A.G., Spits, Hergen, van der Burg, Sjoerd H., and Schumacher, Ton N.M.
- Subjects
Physiological aspects ,Development and progression ,Genetic aspects ,Research ,Tumor antigens -- Physiological aspects -- Genetic aspects -- Research ,Antigenic determinants -- Physiological aspects -- Genetic aspects -- Research ,Melanoma -- Development and progression -- Genetic aspects -- Research ,T cells -- Physiological aspects -- Genetic aspects -- Research - Abstract
Here, we exploit oncogene-immortalized autologous B cells to measure the occurrence of [CD4.sup.+] T-cell responses against melanoma mutanomes identified by tumor exome sequencing. Using this approach, we detected neo-antigen-reactive [CD4.sup.+] [...], Tumor-specific neo-antigens that arise as a consequence of mutations (1,2) are thought to be important for the therapeutic efficacy of cancer immunotherapies (3-5). Accumulating evidence suggests that neo-antigens may be commonly recognized by intratumoral [CD8.sup.+] T cells (3-7), but it is unclear whether neoantigen-specific [CD4.sup.+] T cells also frequently reside within human tumors. In view of the accepted role of tumor-specific [CD4.sup.+] T-cell responses in tumor control (8-10), we addressed whether neo-antigen-specific [CD4.sup.+] T-cell reactivity is a common property in human melanoma.
- Published
- 2015