1. Lymphoid priming in human bone marrow begins before expression of CD10 with upregulation of L-selectin
- Author
-
Kohn, Lisa A, Hao, Qian-Lin, Sasidharan, Rajkumar, Parekh, Chintan, Ge, Shundi, Zhu, Yuhua, Mikkola, Hanna KA, and Crooks, Gay M
- Subjects
Biomedical and Clinical Sciences ,Cardiovascular Medicine and Haematology ,Stem Cell Research - Nonembryonic - Human ,Stem Cell Research ,Antigens ,CD34 ,Antigens ,CD7 ,Bone Marrow Cells ,Cell Differentiation ,Cell Lineage ,Cells ,Cultured ,Gene Expression Profiling ,Hematopoietic Stem Cells ,Humans ,L-Selectin ,Neprilysin ,Thymocytes ,Thymus Gland ,Up-Regulation ,Immunology ,Biochemistry and cell biology - Abstract
Expression of the cell-surface antigen CD10 has long been used to define the lymphoid commitment of human cells. Here we report a unique lymphoid-primed population in human bone marrow that was generated from hematopoietic stem cells (HSCs) before onset of the expression of CD10 and commitment to the B cell lineage. We identified this subset by high expression of the homing molecule L-selectin (CD62L). CD10(-)CD62L(hi) progenitors had full lymphoid and monocytic potential but lacked erythroid potential. Gene-expression profiling placed the CD10(-)CD62L(hi) population at an intermediate stage of differentiation between HSCs and lineage-negative (Lin(-)) CD34(+)CD10(+) progenitors. CD62L was expressed on immature thymocytes, and its ligands were expressed at the cortico-medullary junction of the thymus, which suggested a possible role for this molecule in homing to the thymus. Our studies identify the earliest stage of lymphoid priming in human bone marrow.
- Published
- 2012