1. SARS-CoV-2 antigen exposure history shapes phenotypes and specificity of memory CD8
- Author
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Anastasia A, Minervina, Mikhail V, Pogorelyy, Allison M, Kirk, Jeremy Chase, Crawford, E Kaitlynn, Allen, Ching-Heng, Chou, Robert C, Mettelman, Kim J, Allison, Chun-Yang, Lin, David C, Brice, Xun, Zhu, Kasi, Vegesana, Gang, Wu, Sanchit, Trivedi, Pratibha, Kottapalli, Daniel, Darnell, Suzanne, McNeely, Scott R, Olsen, Stacey, Schultz-Cherry, Jeremie H, Estepp, Maureen A, McGargill, Joshua, Wolf, and Kendall, Whitt
- Subjects
Vaccines, Synthetic ,Phenotype ,SARS-CoV-2 ,Spike Glycoprotein, Coronavirus ,Receptors, Antigen, T-Cell ,COVID-19 ,Humans ,mRNA Vaccines ,CD8-Positive T-Lymphocytes - Abstract
Although mRNA vaccine efficacy against severe coronavirus disease 2019 remains high, variant emergence has prompted booster immunizations. However, the effects of repeated exposures to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigens on memory T cells are poorly understood. Here, we utilize major histocompatibility complex multimers with single-cell RNA sequencing to profile SARS-CoV-2-responsive T cells ex vivo from humans with one, two or three antigen exposures, including vaccination, primary infection and breakthrough infection. Exposure order determined the distribution between spike-specific and non-spike-specific responses, with vaccination after infection leading to expansion of spike-specific T cells and differentiation to CCR7
- Published
- 2021