8 results on '"Yunshun Chen"'
Search Results
2. Mammary tumour cells remodel the bone marrow vascular microenvironment to support metastasis
- Author
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Raymond K. H. Yip, Joel S. Rimes, Bianca D. Capaldo, François Vaillant, Kellie A. Mouchemore, Bhupinder Pal, Yunshun Chen, Elliot Surgenor, Andrew J. Murphy, Robin L. Anderson, Gordon K. Smyth, Geoffrey J. Lindeman, Edwin D. Hawkins, and Jane E. Visvader
- Subjects
Science - Abstract
The visualisation of the bone metastasis process in a spatial temporal manner is lacking. Here, the authors use three-dimensional quantitative imaging and show that mouse mammary tumour cells preferentially home to endothelial subtype type H vessels within the bone marrow and remodel this vasculature by producing granulocyte-colony stimulating factor.
- Published
- 2021
- Full Text
- View/download PDF
3. Construction of developmental lineage relationships in the mouse mammary gland by single-cell RNA profiling
- Author
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Bhupinder Pal, Yunshun Chen, François Vaillant, Paul Jamieson, Lavinia Gordon, Anne C. Rios, Stephen Wilcox, Naiyang Fu, Kevin He Liu, Felicity C. Jackling, Melissa J. Davis, Geoffrey J. Lindeman, Gordon K. Smyth, and Jane E. Visvader
- Subjects
Science - Abstract
The mammary epithelium comprises two cell lineages but the heterogeneity amongst these during development is unclear. Here, the authors report single-cell RNA sequencing of the mouse mammary epithelium at four developmental stages, revealing diversity in both compartments and a transcriptional shift with puberty onset.
- Published
- 2017
- Full Text
- View/download PDF
4. Mammary tumour cells remodel the bone marrow vascular microenvironment to support metastasis
- Author
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Geoffrey J. Lindeman, Kellie A. Mouchemore, Robin L. Anderson, Yunshun Chen, Elliot Surgenor, Bhupinder Pal, Joel S. Rimes, Raymond K H Yip, Andrew J. Murphy, Edwin D. Hawkins, Bianca D. Capaldo, Gordon K. Smyth, François Vaillant, and Jane E. Visvader
- Subjects
Science ,General Physics and Astronomy ,Bone Neoplasms ,Breast Neoplasms ,Mammary Neoplasms, Animal ,Biology ,Cellular imaging ,General Biochemistry, Genetics and Molecular Biology ,Article ,Bone and Bones ,Metastasis ,Mice ,Breast cancer ,Imaging, Three-Dimensional ,Bone Marrow ,Granulocyte Colony-Stimulating Factor ,Receptors, Colony-Stimulating Factor ,medicine ,Tumor Microenvironment ,Animals ,Humans ,Neoplasm Metastasis ,Receptor ,Multidisciplinary ,Bone metastasis ,Neoplasms, Second Primary ,General Chemistry ,medicine.disease ,Haematopoiesis ,medicine.anatomical_structure ,Cancer cell ,Cancer research ,Disease Progression ,Bone marrow ,Blood vessel - Abstract
Bone marrow is a preferred metastatic site for multiple solid tumours and is associated with poor prognosis and significant morbidity. Accumulating evidence indicates that cancer cells colonise specialised niches within the bone marrow to support their long-term propagation, but the precise location and mechanisms that mediate niche interactions are unknown. Using breast cancer as a model of solid tumour metastasis to the bone marrow, we applied large-scale quantitative three-dimensional imaging to characterise temporal changes in the bone marrow microenvironment during disease progression. We show that mouse mammary tumour cells preferentially home to a pre-existing metaphyseal domain enriched for type H vessels. Metastatic lesion outgrowth rapidly remodelled the local vasculature through extensive sprouting to establish a tumour-supportive microenvironment. The evolution of this tumour microenvironment reflects direct remodelling of the vascular endothelium through tumour-derived granulocyte-colony stimulating factor (G-CSF) in a hematopoietic cell-independent manner. Therapeutic targeting of the metastatic niche by blocking G-CSF receptor inhibited pathological blood vessel remodelling and reduced bone metastasis burden. These findings elucidate a mechanism of ‘host’ microenvironment hijacking by mammary tumour cells to subvert the local microvasculature to form a specialised, pro-tumorigenic niche., The visualisation of the bone metastasis process in a spatial temporal manner is lacking. Here, the authors use three-dimensional quantitative imaging and show that mouse mammary tumour cells preferentially home to endothelial subtype type H vessels within the bone marrow and remodel this vasculature by producing granulocyte-colony stimulating factor.
- Published
- 2021
5. Barcoding reveals complex clonal behavior in patient-derived xenografts of metastatic triple negative breast cancer
- Author
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Tom S. Weber, François Vaillant, Jane E. Visvader, Dawn S. Lin, Ton N. Schumacher, Gordon K. Smyth, Daniel L Cameron, L Di Stefano, Bhupinder Pal, Anthony T. Papenfuss, Antonin Serrano, Shalin H. Naik, Delphine Merino, Yunshun Chen, Geoffrey J. Lindeman, Kevin H. Liu, Jaring Schreuder, and Marie Liesse Asselin-Labat
- Subjects
0301 basic medicine ,Science ,General Physics and Astronomy ,02 engineering and technology ,Drug resistance ,Biology ,medicine.disease_cause ,Article ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,medicine ,In patient ,lcsh:Science ,Triple negative ,Triple-negative breast cancer ,Regulation of gene expression ,Cisplatin ,Mutation ,Multidisciplinary ,General Chemistry ,Triple Negative Breast Neoplasms ,021001 nanoscience & nanotechnology ,3. Good health ,030104 developmental biology ,Cancer research ,lcsh:Q ,0210 nano-technology ,medicine.drug - Abstract
Primary triple negative breast cancers (TNBC) are prone to dissemination but sub-clonal relationships between tumors and resulting metastases are poorly understood. Here we use cellular barcoding of two treatment-naïve TNBC patient-derived xenografts (PDXs) to track the spatio-temporal fate of thousands of barcoded clones in primary tumors, and their metastases. Tumor resection had a major impact on reducing clonal diversity in secondary sites, indicating that most disseminated tumor cells lacked the capacity to ‘seed’, hence originated from ‘shedders’ that did not persist. The few clones that continued to grow after resection i.e. ‘seeders’, did not correlate in frequency with their parental clones in primary tumors. Cisplatin treatment of one BRCA1-mutated PDX model to non-palpable levels had a surprisingly minor impact on clonal diversity in the relapsed tumor yet purged 50% of distal clones. Therefore, clonal features of shedding, seeding and drug resistance are important factors to consider for the design of therapeutic strategies., Triple negative breast cancers (TNBC) disseminate and metastasise, but the clonal relationship of metastases to primary tumours is poorly understood. Here, the authors use cellular barcoding of TNBC patient-derived xenografts and track the fate of barcoded clones in primary tumours and their metastases, including after resection or chemotherapy.
- Published
- 2019
6. Publisher Correction: Barcoding reveals complex clonal behavior in patient-derived xenografts of metastatic triple negative breast cancer
- Author
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Jaring Schreuder, L Di Stefano, Yunshun Chen, Kevin H. Liu, Tom S. Weber, Jane E. Visvader, M-L Asselin-Labat, Bhupinder Pal, Anthony T. Papenfuss, François Vaillant, Dawn S. Lin, Daniel L Cameron, Antonin Serrano, Shalin H. Naik, Delphine Merino, Gordon K. Smyth, GJ Lindeman, and Ton N. Schumacher
- Subjects
Oncology ,medicine.medical_specialty ,Tumour heterogeneity ,Science ,ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION ,General Physics and Astronomy ,Triple Negative Breast Neoplasms ,General Biochemistry, Genetics and Molecular Biology ,GeneralLiterature_MISCELLANEOUS ,Mice ,Text mining ,Breast cancer ,Histogram ,Internal medicine ,Cell Line, Tumor ,medicine ,Animals ,Humans ,In patient ,lcsh:Science ,Triple-negative breast cancer ,Multidisciplinary ,business.industry ,BRCA1 Protein ,General Chemistry ,medicine.disease ,Publisher Correction ,Xenograft Model Antitumor Assays ,Clone Cells ,Gene Expression Regulation, Neoplastic ,Disease Models, Animal ,Mutation ,Female ,lcsh:Q ,Cisplatin ,Neoplasm Recurrence, Local ,business ,Genetic techniques - Abstract
Primary triple negative breast cancers (TNBC) are prone to dissemination but sub-clonal relationships between tumors and resulting metastases are poorly understood. Here we use cellular barcoding of two treatment-naïve TNBC patient-derived xenografts (PDXs) to track the spatio-temporal fate of thousands of barcoded clones in primary tumors, and their metastases. Tumor resection had a major impact on reducing clonal diversity in secondary sites, indicating that most disseminated tumor cells lacked the capacity to 'seed', hence originated from 'shedders' that did not persist. The few clones that continued to grow after resection i.e. 'seeders', did not correlate in frequency with their parental clones in primary tumors. Cisplatin treatment of one BRCA1-mutated PDX model to non-palpable levels had a surprisingly minor impact on clonal diversity in the relapsed tumor yet purged 50% of distal clones. Therefore, clonal features of shedding, seeding and drug resistance are important factors to consider for the design of therapeutic strategies.
- Published
- 2019
7. Construction of developmental lineage relationships in the mouse mammary gland by single-cell RNA profiling
- Author
-
Felicity C. Jackling, Yunshun Chen, François Vaillant, Lavinia Gordon, Bhupinder Pal, Stephen Wilcox, Geoffrey J. Lindeman, Melissa J. Davis, Paul R. Jamieson, Gordon K. Smyth, Nai Yang Fu, Jane E. Visvader, Anne C. Rios, and Kevin H. Liu
- Subjects
0301 basic medicine ,Lineage (genetic) ,Science ,Population ,Mammary gland ,Morphogenesis ,General Physics and Astronomy ,Biology ,General Biochemistry, Genetics and Molecular Biology ,Article ,Transcriptome ,03 medical and health sciences ,Mice ,Mammary Glands, Animal ,Gene expression ,medicine ,Animals ,Cell Lineage ,lcsh:Science ,education ,education.field_of_study ,Multidisciplinary ,CD55 Antigens ,Gene Expression Profiling ,Epithelial Cells ,General Chemistry ,Molecular biology ,Cell biology ,Gene expression profiling ,030104 developmental biology ,medicine.anatomical_structure ,Mammary Epithelium ,RNA ,lcsh:Q ,Female ,Single-Cell Analysis - Abstract
The mammary epithelium comprises two primary cellular lineages, but the degree of heterogeneity within these compartments and their lineage relationships during development remain an open question. Here we report single-cell RNA profiling of mouse mammary epithelial cells spanning four developmental stages in the post-natal gland. Notably, the epithelium undergoes a large-scale shift in gene expression from a relatively homogeneous basal-like program in pre-puberty to distinct lineage-restricted programs in puberty. Interrogation of single-cell transcriptomes reveals different levels of diversity within the luminal and basal compartments, and identifies an early progenitor subset marked by CD55. Moreover, we uncover a luminal transit population and a rare mixed-lineage cluster amongst basal cells in the adult mammary gland. Together these findings point to a developmental hierarchy in which a basal-like gene expression program prevails in the early post-natal gland prior to the specification of distinct lineage signatures, and the presence of cellular intermediates that may serve as transit or lineage-primed cells., The mammary epithelium comprises two cell lineages but the heterogeneity amongst these during development is unclear. Here, the authors report single-cell RNA sequencing of the mouse mammary epithelium at four developmental stages, revealing diversity in both compartments and a transcriptional shift with puberty onset.
- Published
- 2017
8. Construction of developmental lineage relationships in the mouse mammary gland by single-cell RNA profiling.
- Author
-
Pal, Bhupinder, Yunshun Chen, Vaillant, François, Jamieson, Paul, Gordon, Lavinia, Rios, Anne C., Wilcox, Stephen, Naiyang Fu, Kevin He Liu, Jackling, Felicity C., Davis, Melissa J., Lindeman, Geoffrey J., Smyth, Gordon K., and Visvader, Jane E.
- Subjects
MAMMARY glands ,RNA ,EPITHELIAL cells ,MICE ,GENE expression - Abstract
The mammary epithelium comprises two primary cellular lineages, but the degree of heterogeneity within these compartments and their lineage relationships during development remain an open question. Here we report single-cell RNA profiling of mouse mammary epithelial cells spanning four developmental stages in the post-natal gland. Notably, the epithelium undergoes a large-scale shift in gene expression from a relatively homogeneous basal-like program in pre-puberty to distinct lineage-restricted programs in puberty. Interrogation of single-cell transcriptomes reveals different levels of diversity within the luminal and basal compartments, and identifies an early progenitor subset marked by CD55. Moreover, we uncover a luminal transit population and a rare mixed-lineage cluster amongst basal cells in the adult mammary gland. Together these findings point to a developmental hierarchy in which a basal-like gene expression program prevails in the early post-natal gland prior to the specification of distinct lineage signatures, and the presence of cellular intermediates that may serve as transit or lineage-primed cells. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
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