1. Stabilization of dimeric PYR/PYL/RCAR family members relieves abscisic acid-induced inhibition of seed germination
- Author
-
Zhi-Zheng Wang, Min-Jie Cao, Junjie Yan, Jin Dong, Mo-Xian Chen, Jing-Fang Yang, Jian-Hong Li, Rui-Ning Ying, Yang-Yang Gao, Li Li, Ya-Nan Leng, Yuan Tian, Kamalani Achala H. Hewage, Rong-Jie Pei, Zhi-You Huang, Ping Yin, Jian-Kang Zhu, Ge-Fei Hao, and Guang-Fu Yang
- Subjects
Science - Abstract
Abstract Abscisic acid (ABA) is the primary preventing factor of seed germination, which is crucial to plant survival and propagation. ABA-induced seed germination inhibition is mainly mediated by the dimeric PYR/PYL/RCAR (PYLs) family members. However, little is known about the relevance between dimeric stability of PYLs and seed germination. Here, we reveal that stabilization of PYL dimer can relieve ABA-induced inhibition of seed germination using chemical genetic approaches. Di-nitrobensulfamide (DBSA), a computationally designed chemical probe, yields around ten-fold improvement in receptor affinity relative to ABA. DBSA reverses ABA-induced inhibition of seed germination mainly through dimeric receptors and recovers the expression of ABA-responsive genes. DBSA maintains PYR1 in dimeric state during protein oligomeric state experiment. X-ray crystallography shows that DBSA targets a pocket in PYL dimer interface and may stabilize PYL dimer by forming hydrogen networks. Our results illustrate the potential of PYL dimer stabilization in preventing ABA-induced seed germination inhibition.
- Published
- 2024
- Full Text
- View/download PDF