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16 results on '"Illing P"'

1. Mapping the immunopeptidome of seven SARS-CoV-2 antigens across common HLA haplotypes

Author

Braun, Asolina, Rowntree, Louise C., Huang, Ziyi, Pandey, Kirti, Thuesen, Nikolas, Li, Chen, Petersen, Jan, Littler, Dene R., Raji, Shabana, Nguyen, Thi H. O., Jappe Lange, Emma, Persson, Gry, Schantz Klausen, Michael, Kringelum, Jens, Chung, Shanzou, Croft, Nathan P., Faridi, Pouya, Ayala, Rochelle, Rossjohn, Jamie, Illing, Patricia T., Scull, Katherine E., Ramarathinam, Sri, Mifsud, Nicole A., Kedzierska, Katherine, Sørensen, Anders Bundgård, and Purcell, Anthony W.

Published
2024
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2. CD8+ T-cell responses towards conserved influenza B virus epitopes across anatomical sites and age

Author

Menon, Tejas, Illing, Patricia T., Chaurasia, Priyanka, McQuilten, Hayley A., Shepherd, Chloe, Rowntree, Louise C., Petersen, Jan, Littler, Dene R., Khuu, Grace, Huang, Ziyi, Allen, Lilith F., Rockman, Steve, Crowe, Jane, Flanagan, Katie L., Wakim, Linda M., Nguyen, Thi H. O., Mifsud, Nicole A., Rossjohn, Jamie, Purcell, Anthony W., van de Sandt, Carolien E., and Kedzierska, Katherine

Published
2024
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3. Mapping the immunopeptidome of seven SARS-CoV-2 antigens across common HLA haplotypes

Author

Asolina Braun, Louise C. Rowntree, Ziyi Huang, Kirti Pandey, Nikolas Thuesen, Chen Li, Jan Petersen, Dene R. Littler, Shabana Raji, Thi H. O. Nguyen, Emma Jappe Lange, Gry Persson, Michael Schantz Klausen, Jens Kringelum, Shanzou Chung, Nathan P. Croft, Pouya Faridi, Rochelle Ayala, Jamie Rossjohn, Patricia T. Illing, Katherine E. Scull, Sri Ramarathinam, Nicole A. Mifsud, Katherine Kedzierska, Anders Bundgård Sørensen, and Anthony W. Purcell

Subjects
Science
Abstract

Abstract Most COVID-19 vaccines elicit immunity against the SARS-CoV-2 Spike protein. However, Spike protein mutations in emerging strains and immune evasion by the SARS-CoV-2 virus demonstrates the need to develop more broadly targeting vaccines. To facilitate this, we use mass spectrometry to identify immunopeptides derived from seven relatively conserved structural and non-structural SARS-CoV-2 proteins (N, E, Nsp1/4/5/8/9). We use two different B-lymphoblastoid cell lines to map Human Leukocyte Antigen (HLA) class I and class II immunopeptidomes covering some of the prevalent HLA types across the global human population. We employ DNA plasmid transfection and direct antigen delivery approaches to sample different antigens and find 248 unique HLA class I and HLA class II bound peptides with 71 derived from N, 12 from E, 28 from Nsp1, 19 from Nsp4, 73 from Nsp8 and 45 peptides derived from Nsp9. Over half of the viral peptides are unpublished. T cell reactivity tested against 56 of the detected peptides shows CD8+ and CD4+ T cell responses against several peptides from the N, E, and Nsp9 proteins. Results from this study will aid the development of next-generation COVID vaccines targeting epitopes from across a number of SARS-CoV-2 proteins.

Published
2024
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4. CD8+ T-cell responses towards conserved influenza B virus epitopes across anatomical sites and age

Author

Tejas Menon, Patricia T. Illing, Priyanka Chaurasia, Hayley A. McQuilten, Chloe Shepherd, Louise C. Rowntree, Jan Petersen, Dene R. Littler, Grace Khuu, Ziyi Huang, Lilith F. Allen, Steve Rockman, Jane Crowe, Katie L. Flanagan, Linda M. Wakim, Thi H. O. Nguyen, Nicole A. Mifsud, Jamie Rossjohn, Anthony W. Purcell, Carolien E. van de Sandt, and Katherine Kedzierska

Subjects
Science
Abstract

Abstract Influenza B viruses (IBVs) cause substantive morbidity and mortality, and yet immunity towards IBVs remains understudied. CD8+ T-cells provide broadly cross-reactive immunity and alleviate disease severity by recognizing conserved epitopes. Despite the IBV burden, only 18 IBV-specific T-cell epitopes restricted by 5 HLAs have been identified currently. A broader array of conserved IBV T-cell epitopes is needed to develop effective cross-reactive T-cell based IBV vaccines. Here we identify 9 highly conserved IBV CD8+ T-cell epitopes restricted to HLA-B*07:02, HLA-B*08:01 and HLA-B*35:01. Memory IBV-specific tetramer+CD8+ T-cells are present within blood and tissues. Frequencies of IBV-specific CD8+ T-cells decline with age, but maintain a central memory phenotype. HLA-B*07:02 and HLA-B*08:01-restricted NP30-38 epitope-specific T-cells have distinct T-cell receptor repertoires. We provide structural basis for the IBV HLA-B*07:02-restricted NS1196-206 (11-mer) and HLA-B*07:02-restricted NP30-38 epitope presentation. Our study increases the number of IBV CD8+ T-cell epitopes, and defines IBV-specific CD8+ T-cells at cellular and molecular levels, across tissues and age.

Published
2024
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5. Self-healable printed magnetic field sensors using alternating magnetic fields

Author

Rui Xu, Gilbert Santiago Cañón Bermúdez, Oleksandr V. Pylypovskyi, Oleksii M. Volkov, Eduardo Sergio Oliveros Mata, Yevhen Zabila, Rico Illing, Pavlo Makushko, Pavel Milkin, Leonid Ionov, Jürgen Fassbender, and Denys Makarov

Subjects
Science
Abstract

Flexible magnetic sensors with high sensitivity have a wide variety of medical and industrial uses, however, making such sensors robust and flexible at the same time can be challenging. Here, the authors demonstrate a high sensitivity flexible magnetic sensor that exhibits self-healing under an applied alternative magnetic field, with complete performance recovery.

Published
2022
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8. Limiting motorboat noise on coral reefs boosts fish reproductive success

Author

Sophie L. Nedelec, Andrew N. Radford, Peter Gatenby, Isla Keesje Davidson, Laura Velasquez Jimenez, Maggie Travis, Katherine E. Chapman, Kieran P. McCloskey, Timothy A. C. Lamont, Björn Illing, Mark I. McCormick, and Stephen D. Simpson

Subjects
Science
Abstract

Using a season-long field manipulation with an established model fish system on the Great Barrier Reef, this study demonstrates that limiting motorboat activity on reefs leads to faster growth and survival of more fish offspring compared to reefs experiencing busy motorboat traffic. Noise mitigation and abatement could therefore present a valuable opportunity for enhancing ecosystem resilience.

Published
2022
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9. CD8+ T cell landscape in Indigenous and non-Indigenous people restricted by influenza mortality-associated HLA-A*24:02 allomorph

Author

Luca Hensen, Patricia T. Illing, E. Bridie Clemens, Thi H. O. Nguyen, Marios Koutsakos, Carolien E. van de Sandt, Nicole A. Mifsud, Andrea T. Nguyen, Christopher Szeto, Brendon Y. Chua, Hanim Halim, Simone Rizzetto, Fabio Luciani, Liyen Loh, Emma J. Grant, Phillipa M. Saunders, Andrew G. Brooks, Steve Rockman, Tom C. Kotsimbos, Allen C. Cheng, Michael Richards, Glen P. Westall, Linda M. Wakim, Thomas Loudovaris, Stuart I. Mannering, Michael Elliott, Stuart G. Tangye, David C. Jackson, Katie L. Flanagan, Jamie Rossjohn, Stephanie Gras, Jane Davies, Adrian Miller, Steven Y. C. Tong, Anthony W. Purcell, and Katherine Kedzierska

Subjects
Science
Abstract

The immunology of Indigenous populations is generally understudied outside the context of diseases that are prevalent in these communities. Here the authors identify prevalence of influenza CD8+ T cell epitopes in an Indigenous Australian population expressing the susceptibility allomorph HLA A*24:02 and validate immunodominance of some of these epitopes in mice.

Published
2021
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10. Thermostability profiling of MHC-bound peptides: a new dimension in immunopeptidomics and aid for immunotherapy design

Author

Emma C. Jappe, Christian Garde, Sri H. Ramarathinam, Ethan Passantino, Patricia T. Illing, Nicole A. Mifsud, Thomas Trolle, Jens V. Kringelum, Nathan P. Croft, and Anthony W. Purcell

Subjects
Science
Abstract

Thermostability of the peptide-MHC interaction is important for immunogenicity. Here the authors present a mass spectrometry method to measure thermostability among thousands of peptide-MHC complexes in parallel and a trained artificial neural network to predict immunogenenicity of cancer antigens.

Published
2020
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11. CD8+ T cell landscape in Indigenous and non-Indigenous people restricted by influenza mortality-associated HLA-A*24:02 allomorph

Author

Hensen, Luca, Illing, Patricia T., Bridie Clemens, E., Nguyen, Thi H. O., Koutsakos, Marios, van de Sandt, Carolien E., Mifsud, Nicole A., Nguyen, Andrea T., Szeto, Christopher, Chua, Brendon Y., Halim, Hanim, Rizzetto, Simone, Luciani, Fabio, Loh, Liyen, Grant, Emma J., Saunders, Phillipa M., Brooks, Andrew G., Rockman, Steve, Kotsimbos, Tom C., Cheng, Allen C., Richards, Michael, Westall, Glen P., Wakim, Linda M., Loudovaris, Thomas, Mannering, Stuart I., Elliott, Michael, Tangye, Stuart G., Jackson, David C., Flanagan, Katie L., Rossjohn, Jamie, Gras, Stephanie, Davies, Jane, Miller, Adrian, Tong, Steven Y. C., Purcell, Anthony W., and Kedzierska, Katherine

Published
2021
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12. A bimodal soft electronic skin for tactile and touchless interaction in real time

Author

Jin Ge, Xu Wang, Michael Drack, Oleksii Volkov, Mo Liang, Gilbert Santiago Cañón Bermúdez, Rico Illing, Changan Wang, Shengqiang Zhou, Jürgen Fassbender, Martin Kaltenbrunner, and Denys Makarov

Subjects
Science
Abstract

To realize electronic skins for emerging technologies that require multifunctional sensing capability, intelligent design strategies are needed. Here, the authors report electronic skins with a single sensory unit that simultaneously transduces both tactile and touchless stimulations.

Published
2019
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14. HLA-B57 micropolymorphism defines the sequence and conformational breadth of the immunopeptidome

Author

Patricia T. Illing, Phillip Pymm, Nathan P. Croft, Hugo G. Hilton, Vladimir Jojic, Alex S. Han, Juan L. Mendoza, Nicole A. Mifsud, Nadine L. Dudek, James McCluskey, Peter Parham, Jamie Rossjohn, Julian P. Vivian, and Anthony W. Purcell

Subjects
Science
Abstract

Human leukocyte antigens (HLA) are multi-allelic and polymorphic genes that present antigens to immune cells for inducing protective immunity. Here, using systems biology and structural approaches, the authors show that micropolymorphism of three HLA has effects beyond the modulation of antigen diversity.

Published
2018
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