1. An expression atlas of variant ionotropic glutamate receptors identifies a molecular basis of carbonation sensing
- Author
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Kathrin Steck, Juan Antonio Sánchez-Alcañiz, Anantha Krishna Sivasubramaniam, Ana F. Silbering, Giovanna Zappia, Vincent Croset, Richard Benton, Daniel Münch, Saumya Yashmohini Sahai, G. Larisa Neagu-Maier, Nilay Yapici, Liliane Abuin, Simon G. Sprecher, Carlos Ribeiro, Steeve Cruchet, and Thomas O. Auer
- Subjects
0301 basic medicine ,Taste ,Carbonation ,Science ,Carbonates ,General Physics and Astronomy ,Sensory system ,Receptors, Ionotropic Glutamate ,Article ,General Biochemistry, Genetics and Molecular Biology ,Animals ,Behavior, Animal/physiology ,Carbonates/metabolism ,Drosophila Proteins/genetics ,Drosophila Proteins/metabolism ,Drosophila melanogaster ,Fatty Acids/metabolism ,Neurons/cytology ,Neurons/metabolism ,Receptors, Ionotropic Glutamate/genetics ,Receptors, Ionotropic Glutamate/metabolism ,Taste/genetics ,Taste/physiology ,03 medical and health sciences ,0302 clinical medicine ,Genetics ,medicine ,Drosophila Proteins ,Receptor ,lcsh:Science ,Neurons ,Multidisciplinary ,Behavior, Animal ,biology ,Chemistry ,Fatty Acids ,fungi ,Glutamate receptor ,General Chemistry ,biology.organism_classification ,Publisher Correction ,Cell biology ,030104 developmental biology ,medicine.anatomical_structure ,lcsh:Q ,Neuron ,030217 neurology & neurosurgery ,Neuroscience ,Ionotropic effect - Abstract
Through analysis of the Drosophila ionotropic receptors (IRs), a family of variant ionotropic glutamate receptors, we reveal that most IRs are expressed in peripheral neuron populations in diverse gustatory organs in larvae and adults. We characterise IR56d, which defines two anatomically-distinct neuron classes in the proboscis: one responds to carbonated solutions and fatty acids while the other represents a subset of sugar- and fatty acid-sensing cells. Mutational analysis indicates that IR56d, together with the broadly-expressed co-receptors IR25a and IR76b, is essential for physiological responses to carbonation and fatty acids, but not sugars. We further demonstrate that carbonation and fatty acids both promote IR56d-dependent attraction of flies, but through different behavioural outputs. Our work provides a toolkit for investigating taste functions of IRs, defines a subset of these receptors required for carbonation sensing, and illustrates how the gustatory system uses combinatorial expression of sensory molecules in distinct neurons to coordinate behaviour., Little is known about the role of variant ionotropic glutamate receptors (IRs) in insect taste. Here the authors characterise the expression pattern of IRs in the Drosophila gustatory system and highlight the role of one receptor, IR56d, in the detection of carbonation
- Published
- 2018