18 results on '"Cong, Lin"'
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2. Fully automatic transfer and measurement system for structural superlubric materials
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Li Chen, Cong Lin, Diwei Shi, Xuanyu Huang, Quanshui Zheng, Jinhui Nie, and Ming Ma
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Science - Abstract
Abstract Structural superlubricity, a state of nearly zero friction and no wear between two contact surfaces under relative sliding, holds immense potential for research and application prospects in micro-electro-mechanical systems devices, mechanical engineering, and energy resources. A critical step towards the practical application of structural superlubricity is the mass transfer and high throughput performance evaluation. Limited by the yield rate of material preparation, existing automated systems, such as roll printing or massive stamping, are inadequate for this task. In this paper, a machine learning-assisted system is proposed to realize fully automated selective transfer and tribological performance measurement for structural superlubricity materials. Specifically, the system has a judgment accuracy of over 98% for the selection of micro-scale graphite flakes with structural superlubricity properties and complete the 100 graphite flakes assembly array to form various pre-designed patterns within 100 mins, which is 15 times faster than manual operation. Besides, the system is capable of automatically measuring the tribological performance of over 100 selected flakes on Si3N4, delivering statistical results for new interface which is beyond the reach of traditional methods. With its high accuracy, efficiency, and robustness, this machine learning-assisted system promotes the fundamental research and practical application of structural superlubricity.
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- 2023
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3. Revealing the aging process of solid electrolyte interphase on SiOx anode
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Guoyu Qian, Yiwei Li, Haibiao Chen, Lin Xie, Tongchao Liu, Ni Yang, Yongli Song, Cong Lin, Junfang Cheng, Naotoshi Nakashima, Meng Zhang, Zikun Li, Wenguang Zhao, Xiangjie Yang, Hai Lin, Xia Lu, Luyi Yang, Hong Li, Khalil Amine, Liquan Chen, and Feng Pan
- Subjects
Science - Abstract
Abstract As one of the most promising alternatives to graphite negative electrodes, silicon oxide (SiO x ) has been hindered by its fast capacity fading. Solid electrolyte interphase (SEI) aging on silicon SiO x has been recognized as the most critical yet least understood facet. Herein, leveraging 3D focused ion beam-scanning electron microscopy (FIB-SEM) tomographic imaging, we reveal an exceptionally characteristic SEI microstructure with an incompact inner region and a dense outer region, which overturns the prevailing belief that SEIs are homogeneous structure and reveals the SEI evolution process. Through combining nanoprobe and electron energy loss spectroscopy (EELS), it is also discovered that the electronic conductivity of thick SEI relies on the percolation network within composed of conductive agents (e.g., carbon black particles), which are embedded into the SEI upon its growth. Therefore, the free growth of SEI will gradually attenuate this electron percolation network, thereby causing capacity decay of SiO x . Based on these findings, a proof-of-concept strategy is adopted to mechanically restrict the SEI growth via applying a confining layer on top of the electrode. Through shedding light on the fundamental understanding of SEI aging for SiO x anodes, this work could potentially inspire viable improving strategies in the future.
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- 2023
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4. Age-related self-DNA accumulation may accelerate arthritis in rats and in human rheumatoid arthritis
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Wei-Dan Luo, Yu-Ping Wang, Jun Lv, Yong Liu, Yuan-Qing Qu, Xiong-Fei Xu, Li-Jun Yang, Zi-Cong Lin, Lin-Na Wang, Rui-Hong Chen, Jiu-Jie Yang, Ya-Ling Zeng, Rui-Long Zhang, Bai-Xiong Huang, Xiao-Yun Yun, Xuan-Ying Wang, Lin-Lin Song, Jian-Hui Wu, Xing-Xia Wang, Xi Chen, Wei Zhang, Hui-Miao Wang, Li-Qun Qu, Meng-Han Liu, Liang Liu, Betty Yuen Kwan Law, and Vincent Kam Wai Wong
- Subjects
Science - Abstract
Abstract The incidence of rheumatoid arthritis (RA) is increasing with age. DNA fragments is known to accumulate in certain autoimmune diseases, but the mechanistic relationship among ageing, DNA fragments and RA pathogenesis remain unexplored. Here we show that the accumulation of DNA fragments, increasing with age and regulated by the exonuclease TREX1, promotes abnormal activation of the immune system in an adjuvant‐induced arthritis (AIA) rat model. Local overexpression of TREX1 suppresses synovial inflammation in rats, while conditional genomic deletion of TREX1 in AIA rats result in higher levels of circulating free (cf) DNA and hence abnormal immune activation, leading to more severe symptoms. The dysregulation of the heterodimeric transcription factor AP-1, formed by c-Jun and c-Fos, appear to regulate both TREX1 expression and SASP induction. Thus, our results confirm that DNA fragments are inflammatory mediators, and TREX1, downstream of AP-1, may serve as regulator of cellular immunity in health and in RA.
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- 2023
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5. Shotgun transcriptome, spatial omics, and isothermal profiling of SARS-CoV-2 infection reveals unique host responses, viral diversification, and drug interactions
- Author
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Butler, Daniel, Mozsary, Christopher, Meydan, Cem, Foox, Jonathan, Rosiene, Joel, Shaiber, Alon, Danko, David, Afshinnekoo, Ebrahim, MacKay, Matthew, Sedlazeck, Fritz J, Ivanov, Nikolay A, Sierra, Maria, Pohle, Diana, Zietz, Michael, Gisladottir, Undina, Ramlall, Vijendra, Sholle, Evan T, Schenck, Edward J, Westover, Craig D, Hassan, Ciaran, Ryon, Krista, Young, Benjamin, Bhattacharya, Chandrima, Ng, Dianna L, Granados, Andrea C, Santos, Yale A, Servellita, Venice, Federman, Scot, Ruggiero, Phyllis, Fungtammasan, Arkarachai, Chin, Chen-Shan, Pearson, Nathaniel M, Langhorst, Bradley W, Tanner, Nathan A, Kim, Youngmi, Reeves, Jason W, Hether, Tyler D, Warren, Sarah E, Bailey, Michael, Gawrys, Justyna, Meleshko, Dmitry, Xu, Dong, Couto-Rodriguez, Mara, Nagy-Szakal, Dorottya, Barrows, Joseph, Wells, Heather, O’Hara, Niamh B, Rosenfeld, Jeffrey A, Chen, Ying, Steel, Peter AD, Shemesh, Amos J, Xiang, Jenny, Thierry-Mieg, Jean, Thierry-Mieg, Danielle, Iftner, Angelika, Bezdan, Daniela, Sanchez, Elizabeth, Campion, Thomas R, Sipley, John, Cong, Lin, Craney, Arryn, Velu, Priya, Melnick, Ari M, Shapira, Sagi, Hajirasouliha, Iman, Borczuk, Alain, Iftner, Thomas, Salvatore, Mirella, Loda, Massimo, Westblade, Lars F, Cushing, Melissa, Wu, Shixiu, Levy, Shawn, Chiu, Charles, Schwartz, Robert E, Tatonetti, Nicholas, Rennert, Hanna, Imielinski, Marcin, and Mason, Christopher E
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Biological Sciences ,Biomedical and Clinical Sciences ,Clinical Sciences ,Infectious Diseases ,Vaccine Related ,Biodefense ,Rare Diseases ,Emerging Infectious Diseases ,Clinical Research ,Prevention ,Lung ,Pneumonia & Influenza ,Aetiology ,Development of treatments and therapeutic interventions ,2.1 Biological and endogenous factors ,5.1 Pharmaceuticals ,Infection ,Good Health and Well Being ,Adult ,Aged ,Angiotensin Receptor Antagonists ,Angiotensin-Converting Enzyme Inhibitors ,Antiviral Agents ,COVID-19 ,COVID-19 Nucleic Acid Testing ,Drug Interactions ,Female ,Gene Expression Profiling ,Genome ,Viral ,HLA Antigens ,Host Microbial Interactions ,Humans ,Male ,Middle Aged ,Molecular Diagnostic Techniques ,New York City ,Nucleic Acid Amplification Techniques ,Pandemics ,RNA-Seq ,SARS-CoV-2 ,COVID-19 Drug Treatment - Abstract
In less than nine months, the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) killed over a million people, including >25,000 in New York City (NYC) alone. The COVID-19 pandemic caused by SARS-CoV-2 highlights clinical needs to detect infection, track strain evolution, and identify biomarkers of disease course. To address these challenges, we designed a fast (30-minute) colorimetric test (LAMP) for SARS-CoV-2 infection from naso/oropharyngeal swabs and a large-scale shotgun metatranscriptomics platform (total-RNA-seq) for host, viral, and microbial profiling. We applied these methods to clinical specimens gathered from 669 patients in New York City during the first two months of the outbreak, yielding a broad molecular portrait of the emerging COVID-19 disease. We find significant enrichment of a NYC-distinctive clade of the virus (20C), as well as host responses in interferon, ACE, hematological, and olfaction pathways. In addition, we use 50,821 patient records to find that renin-angiotensin-aldosterone system inhibitors have a protective effect for severe COVID-19 outcomes, unlike similar drugs. Finally, spatial transcriptomic data from COVID-19 patient autopsy tissues reveal distinct ACE2 expression loci, with macrophage and neutrophil infiltration in the lungs. These findings can inform public health and may help develop and drive SARS-CoV-2 diagnostic, prevention, and treatment strategies.
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- 2021
6. The architecture of transmembrane and cytoplasmic juxtamembrane regions of Toll-like receptors
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F. D. Kornilov, A. V. Shabalkina, Cong Lin, P. E. Volynsky, E. F. Kot, A. L. Kayushin, V. A. Lushpa, M. V. Goncharuk, A. S. Arseniev, S. A. Goncharuk, Xiaohui Wang, and K. S. Mineev
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Science - Abstract
Toll-like receptors (TLRs) play a key role in the innate immune system. Here, Kornilov et al. resolve the 3D structures of the membrane-associated parts of four TLRs to reveal properties of the juxta-membrane domain.
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- 2023
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7. Differential IL18 signaling via IL18 receptor and Na-Cl co-transporter discriminating thermogenesis and glucose metabolism regulation
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Xian Zhang, Songyuan Luo, Minjie Wang, Qiongqiong Cao, Zhixin Zhang, Qin Huang, Jie Li, Zhiyong Deng, Tianxiao Liu, Cong-Lin Liu, Mathilde Meppen, Amelie Vromman, Richard A. Flavell, Gökhan S. Hotamışlıgil, Jian Liu, Peter Libby, Zhangsuo Liu, and Guo-Ping Shi
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Science - Abstract
Interleukin-18 (IL18) has a pivotal role in interferon signalling and T cell development, but increasingly recognized as an adipokine that regulates energy metabolism in fat tissue. Authors here dissect the function of IL18 signalling in the adipose compartment by targeted genomic deletion of its two receptors individually and in combination in brown and white adipose tissues.
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- 2022
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8. Atomic-resolution structures from polycrystalline covalent organic frameworks with enhanced cryo-cRED
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Jian Li, Cong Lin, Tianqiong Ma, and Junliang Sun
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Science - Abstract
Structure determination of covalent organic frameworks (COFs) is the key to pushing the development of COF-based materials further but precise determination of the structure of COFs is challenging. Here, the authors develop a universal ab initio structure determination method for polycrystalline 3D COFs using cryo-cRED by combining hierarchical cluster analysis with cryo-EM technique and demonstrate COF structures with atomic preciscion and up to 0.79-angstrom resolution.
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- 2022
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9. Differential IL18 signaling via IL18 receptor and Na-Cl co-transporter discriminating thermogenesis and glucose metabolism regulation
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Zhang, Xian, Luo, Songyuan, Wang, Minjie, Cao, Qiongqiong, Zhang, Zhixin, Huang, Qin, Li, Jie, Deng, Zhiyong, Liu, Tianxiao, Liu, Cong-Lin, Meppen, Mathilde, Vromman, Amelie, Flavell, Richard A., Hotamışlıgil, Gökhan S., Liu, Jian, Libby, Peter, Liu, Zhangsuo, and Shi, Guo-Ping
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- 2022
- Full Text
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10. Modulated structure determination and ion transport mechanism of oxide-ion conductor CeNbO4+δ
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Jian Li, Fengjuan Pan, Shipeng Geng, Cong Lin, Lukas Palatinus, Mathieu Allix, Xiaojun Kuang, Jianhua Lin, and Junliang Sun
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Science - Abstract
Materials with oxygen hyperstoichiometry received great attention in solid oxide fuel cells field because of the low activation energy of interstitial ion migration. Here the authors revealed the relationship between the structure and oxide ion migration for the whole series of CeNbO4+δ compounds.
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- 2020
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11. Na+-H+ exchanger 1 determines atherosclerotic lesion acidification and promotes atherogenesis
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Cong-Lin Liu, Xian Zhang, Jing Liu, Yunzhe Wang, Galina K. Sukhova, Gregory R. Wojtkiewicz, Tianxiao Liu, Rui Tang, Samuel Achilefu, Matthias Nahrendorf, Peter Libby, Junli Guo, Jin-Ying Zhang, and Guo-Ping Shi
- Subjects
Science - Abstract
Na+-H+ exchanger 1 (Nhe1) regulates extracellular pH by extruding protons in exchange for extracellular Na+ . Here, Liu et al. show that Nhe1 promotes the development and acidification of atherosclerotic lesions and that pH-sensitive probes can be used to monitor plaque growth and acidification.
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- 2019
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12. Na+-H+ exchanger 1 determines atherosclerotic lesion acidification and promotes atherogenesis
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Liu, Cong-Lin, Zhang, Xian, Liu, Jing, Wang, Yunzhe, Sukhova, Galina K., Wojtkiewicz, Gregory R., Liu, Tianxiao, Tang, Rui, Achilefu, Samuel, Nahrendorf, Matthias, Libby, Peter, Guo, Junli, Zhang, Jin-Ying, and Shi, Guo-Ping
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- 2019
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13. Modulated structure determination and ion transport mechanism of oxide-ion conductor CeNbO4+δ
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Fengjuan Pan, Jianhua Lin, Cong Lin, Junliang Sun, Mathieu Allix, Jian Li, Shipeng Geng, Lukáš Palatinus, Xiaojun Kuang, Conditions Extrêmes et Matériaux : Haute Température et Irradiation (CEMHTI), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université d'Orléans (UO), and Université d'Orléans (UO)
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Solid-state chemistry ,Materials science ,Science ,Oxide ,General Physics and Astronomy ,chemistry.chemical_element ,Ionic bonding ,02 engineering and technology ,[CHIM.INOR]Chemical Sciences/Inorganic chemistry ,010402 general chemistry ,01 natural sciences ,Oxygen ,General Biochemistry, Genetics and Molecular Biology ,Ion ,chemistry.chemical_compound ,[CHIM.CRIS]Chemical Sciences/Cristallography ,Ionic conductivity ,[CHIM]Chemical Sciences ,lcsh:Science ,Ion transporter ,Multidisciplinary ,General Chemistry ,[CHIM.MATE]Chemical Sciences/Material chemistry ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Electron diffraction ,chemistry ,Physical chemistry ,lcsh:Q ,0210 nano-technology - Abstract
CeNbO4+δ, a family of oxygen hyperstoichiometry materials with varying oxygen content (CeNbO4, CeNbO4.08, CeNbO4.25, CeNbO4.33) that shows mixed electronic and oxide ionic conduction, has been known for four decades. However, the oxide ionic transport mechanism has remained unclear due to the unknown atomic structures of CeNbO4.08 and CeNbO4.33. Here, we report the complex (3 + 1)D incommensurately modulated structure of CeNbO4.08, and the supercell structure of CeNbO4.33 from single nanocrystals by using a three-dimensional electron diffraction technique. Two oxide ion migration events are identified in CeNbO4.08 and CeNbO4.25 by molecular dynamics simulations, which was a synergic-cooperation knock-on mechanism involving continuous breaking and reformation of Nb2O9 units. However, the excess oxygen in CeNbO4.33 hardly migrates because of the high concentration and the ordered distribution of the excess oxide ions. The relationship between the structure and oxide ion migration for the whole series of CeNbO4+δ compounds elucidated here provides a direction for the performance optimization of these compounds.
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- 2020
14. Eosinophils improve cardiac function after myocardial infarction
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Dafeng Yang, Xian Zhang, Dazhu Li, Axel Cosmus Pyndt Diederichsen, Yunzhe Wang, Tianxiao Liu, Zhiyong Deng, Chongzhe Yang, Cong-Lin Liu, Lars Melholt Rasmussen, Jing Liu, Jing Wang, Peter Libby, Francis W. Luscinskas, Gail Newton, Wenqian Fang, Lijun Liu, Qin Huang, Guo-Ping Shi, Galina K. Sukhova, Junli Guo, Jes S. Lindholt, and Jie Li
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0301 basic medicine ,Myocardium/pathology ,Male ,Myocardial Infarction ,General Physics and Astronomy ,030204 cardiovascular system & hematology ,Electrocardiography ,0302 clinical medicine ,Fibrosis ,Medicine ,Myocyte ,Diphtheria Toxin ,Myocytes, Cardiac ,Myocardial infarction ,Mice, Inbred BALB C ,Multidisciplinary ,Cell Death ,Interleukin-4/genetics ,respiratory system ,Endothelial stem cell ,medicine.anatomical_structure ,cardiovascular system ,Diphtheria Toxin/toxicity ,Female ,Cardiac function curve ,Programmed cell death ,Science ,Heart failure ,Mice, Transgenic ,Ribonucleases/genetics ,General Biochemistry, Genetics and Molecular Biology ,Article ,Myocytes, Cardiac/pathology ,03 medical and health sciences ,Ribonucleases ,Animals ,Humans ,Interleukin 4 ,Aged ,Eosinophils/drug effects ,business.industry ,Myocardial Infarction/physiopathology ,Myocardium ,General Chemistry ,Eosinophil ,Fibroblasts ,medicine.disease ,Fibroblasts/pathology ,Eosinophils ,Mice, Inbred C57BL ,030104 developmental biology ,Immunology ,Interleukin-4 ,business - Abstract
Clinical studies reveal changes in blood eosinophil counts and eosinophil cationic proteins that may serve as risk factors for human coronary heart diseases. Here we report an increase of blood or heart eosinophil counts in humans and mice after myocardial infarction (MI), mostly in the infarct region. Genetic or inducible depletion of eosinophils exacerbates cardiac dysfunction, cell death, and fibrosis post-MI, with concurrent acute increase of heart and chronic increase of splenic neutrophils and monocytes. Mechanistic studies reveal roles of eosinophil IL4 and cationic protein mEar1 in blocking H2O2- and hypoxia-induced mouse and human cardiomyocyte death, TGF-β-induced cardiac fibroblast Smad2/3 activation, and TNF-α-induced neutrophil adhesion on the heart endothelial cell monolayer. In vitro-cultured eosinophils from WT mice or recombinant mEar1 protein, but not eosinophils from IL4-deficient mice, effectively correct exacerbated cardiac dysfunctions in eosinophil-deficient ∆dblGATA mice. This study establishes a cardioprotective role of eosinophils in post-MI hearts., Blood eosinophil (EOS) counts may serve as risk factors for human coronary heart diseases. Here the authors show that increased circulating and myocardial EOS after myocardial infarction play a cardioprotective role by reducing cardiomyocyte death, cardiac fibroblast activation and fibrosis, and endothelium activation-mediated inflammatory cell accumulation.
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- 2020
15. Modulated structure determination and ion transport mechanism of oxide-ion conductor CeNbO
- Author
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Jian, Li, Fengjuan, Pan, Shipeng, Geng, Cong, Lin, Lukas, Palatinus, Mathieu, Allix, Xiaojun, Kuang, Jianhua, Lin, and Junliang, Sun
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Chemistry ,Electronic materials ,Materials chemistry ,Solid-state chemistry ,Article ,Inorganic chemistry - Abstract
CeNbO4+δ, a family of oxygen hyperstoichiometry materials with varying oxygen content (CeNbO4, CeNbO4.08, CeNbO4.25, CeNbO4.33) that shows mixed electronic and oxide ionic conduction, has been known for four decades. However, the oxide ionic transport mechanism has remained unclear due to the unknown atomic structures of CeNbO4.08 and CeNbO4.33. Here, we report the complex (3 + 1)D incommensurately modulated structure of CeNbO4.08, and the supercell structure of CeNbO4.33 from single nanocrystals by using a three-dimensional electron diffraction technique. Two oxide ion migration events are identified in CeNbO4.08 and CeNbO4.25 by molecular dynamics simulations, which was a synergic-cooperation knock-on mechanism involving continuous breaking and reformation of Nb2O9 units. However, the excess oxygen in CeNbO4.33 hardly migrates because of the high concentration and the ordered distribution of the excess oxide ions. The relationship between the structure and oxide ion migration for the whole series of CeNbO4+δ compounds elucidated here provides a direction for the performance optimization of these compounds., Materials with oxygen hyperstoichiometry received great attention in solid oxide fuel cells field because of the low activation energy of interstitial ion migration. Here the authors revealed the relationship between the structure and oxide ion migration for the whole series of CeNbO4+δ compounds.
- Published
- 2019
16. Na+-H+ exchanger 1 determines atherosclerotic lesion acidification and promotes atherogenesis
- Author
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Jin Ying Zhang, Samuel Achilefu, Xian Zhang, Cong-Lin Liu, Tianxiao Liu, Rui Tang, Peter Libby, Yunzhe Wang, Jing Liu, Galina K. Sukhova, Guo-Ping Shi, Gregory R. Wojtkiewicz, Junli Guo, and Matthias Nahrendorf
- Subjects
0301 basic medicine ,Science ,General Physics and Astronomy ,02 engineering and technology ,Immunoglobulin E ,General Biochemistry, Genetics and Molecular Biology ,Lesion ,03 medical and health sciences ,medicine ,Extracellular ,Macrophage ,lcsh:Science ,Receptor ,Foam cell ,Multidisciplinary ,biology ,Chemistry ,Colocalization ,General Chemistry ,021001 nanoscience & nanotechnology ,Molecular biology ,3. Good health ,030104 developmental biology ,Apoptosis ,biology.protein ,lcsh:Q ,medicine.symptom ,0210 nano-technology - Abstract
The pH in atherosclerotic lesions varies between individuals. IgE activates macrophage Na+-H+ exchanger (Nhe1) and induces extracellular acidification and cell apoptosis. Here, we show that the pH-sensitive pHrodo probe localizes the acidic regions in atherosclerotic lesions to macrophages, IgE, and cell apoptosis. In Apoe–/– mice, Nhe1-deficiency or anti-IgE antibody reduces atherosclerosis and blocks lesion acidification. Reduced atherosclerosis in Apoe–/– mice receiving bone marrow from Nhe1- or IgE receptor FceR1-deficient mice, blunted foam cell formation and signaling in IgE-activated macrophages from Nhe1-deficient mice, immunocomplex formation of Nhe1 and FceR1 in IgE-activated macrophages, and Nhe1-FceR1 colocalization in atherosclerotic lesion macrophages support a role of IgE-mediated macrophage Nhe1 activation in atherosclerosis. Intravenous administration of a near-infrared fluorescent pH-sensitive probe LS662, followed by coregistered fluorescent molecular tomography-computed tomography imaging, identifies acidic regions in atherosclerotic lesions in live mice, ushering a non-invasive and radiation-free imaging approach to monitor atherosclerotic lesions in live subjects. Na+-H+ exchanger 1 (Nhe1) regulates extracellular pH by extruding protons in exchange for extracellular Na+ . Here, Liu et al. show that Nhe1 promotes the development and acidification of atherosclerotic lesions and that pH-sensitive probes can be used to monitor plaque growth and acidification.
- Published
- 2019
17. Na
- Author
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Cong-Lin, Liu, Xian, Zhang, Jing, Liu, Yunzhe, Wang, Galina K, Sukhova, Gregory R, Wojtkiewicz, Tianxiao, Liu, Rui, Tang, Samuel, Achilefu, Matthias, Nahrendorf, Peter, Libby, Junli, Guo, Jin-Ying, Zhang, and Guo-Ping, Shi
- Subjects
Mice, Knockout ,Sodium-Hydrogen Exchanger 1 ,Macrophages ,Apoptosis ,Hydrogen-Ion Concentration ,Macrophage Activation ,Atherosclerosis ,Plaque, Atherosclerotic ,Article ,Mice, Inbred C57BL ,Apolipoproteins E ,Animals ,Humans ,Foam cells ,Acids ,Signal Transduction - Abstract
The pH in atherosclerotic lesions varies between individuals. IgE activates macrophage Na+-H+ exchanger (Nhe1) and induces extracellular acidification and cell apoptosis. Here, we show that the pH-sensitive pHrodo probe localizes the acidic regions in atherosclerotic lesions to macrophages, IgE, and cell apoptosis. In Apoe–/– mice, Nhe1-deficiency or anti-IgE antibody reduces atherosclerosis and blocks lesion acidification. Reduced atherosclerosis in Apoe–/– mice receiving bone marrow from Nhe1- or IgE receptor FcεR1-deficient mice, blunted foam cell formation and signaling in IgE-activated macrophages from Nhe1-deficient mice, immunocomplex formation of Nhe1 and FcεR1 in IgE-activated macrophages, and Nhe1-FcεR1 colocalization in atherosclerotic lesion macrophages support a role of IgE-mediated macrophage Nhe1 activation in atherosclerosis. Intravenous administration of a near-infrared fluorescent pH-sensitive probe LS662, followed by coregistered fluorescent molecular tomography-computed tomography imaging, identifies acidic regions in atherosclerotic lesions in live mice, ushering a non-invasive and radiation-free imaging approach to monitor atherosclerotic lesions in live subjects., Na+-H+ exchanger 1 (Nhe1) regulates extracellular pH by extruding protons in exchange for extracellular Na+ . Here, Liu et al. show that Nhe1 promotes the development and acidification of atherosclerotic lesions and that pH-sensitive probes can be used to monitor plaque growth and acidification.
- Published
- 2017
18. Na+-H+ exchanger 1 determines atherosclerotic lesion acidification and promotes atherogenesis.
- Author
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Liu, Cong-Lin, Zhang, Xian, Liu, Jing, Wang, Yunzhe, Sukhova, Galina K., Wojtkiewicz, Gregory R., Liu, Tianxiao, Tang, Rui, Achilefu, Samuel, Nahrendorf, Matthias, Libby, Peter, Guo, Junli, Zhang, Jin-Ying, and Shi, Guo-Ping
- Subjects
SODIUM-calcium exchanger ,ACIDIFICATION ,APOPTOSIS ,TISSUE wounds ,ATHEROSCLEROSIS - Abstract
The pH in atherosclerotic lesions varies between individuals. IgE activates macrophage Na
+ -H+ exchanger (Nhe1) and induces extracellular acidification and cell apoptosis. Here, we show that the pH-sensitive pHrodo probe localizes the acidic regions in atherosclerotic lesions to macrophages, IgE, and cell apoptosis. In Apoe–/– mice, Nhe1-deficiency or anti-IgE antibody reduces atherosclerosis and blocks lesion acidification. Reduced atherosclerosis in Apoe–/– mice receiving bone marrow from Nhe1- or IgE receptor FcεR1-deficient mice, blunted foam cell formation and signaling in IgE-activated macrophages from Nhe1-deficient mice, immunocomplex formation of Nhe1 and FcεR1 in IgE-activated macrophages, and Nhe1-FcεR1 colocalization in atherosclerotic lesion macrophages support a role of IgE-mediated macrophage Nhe1 activation in atherosclerosis. Intravenous administration of a near-infrared fluorescent pH-sensitive probe LS662, followed by coregistered fluorescent molecular tomography-computed tomography imaging, identifies acidic regions in atherosclerotic lesions in live mice, ushering a non-invasive and radiation-free imaging approach to monitor atherosclerotic lesions in live subjects. Na+-H+ exchanger 1 (Nhe1) regulates extracellular pH by extruding protons in exchange for extracellular Na+. Here, Liu et al. show that Nhe1 promotes the development and acidification of atherosclerotic lesions and that pH-sensitive probes can be used to monitor plaque growth and acidification. [ABSTRACT FROM AUTHOR]- Published
- 2019
- Full Text
- View/download PDF
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