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Your search keyword '"Aa Haeruman Azam"' showing total 3 results
Start Over Author "Aa Haeruman Azam" Journal nature communications
3 results on '"Aa Haeruman Azam"'

1. Evasion of antiviral bacterial immunity by phage tRNAs

Author

Aa Haeruman Azam, Kohei Kondo, Kotaro Chihara, Tomohiro Nakamura, Shinjiro Ojima, Wenhan Nie, Azumi Tamura, Wakana Yamashita, Yo Sugawara, Motoyuki Sugai, Longzhu Cui, Yoshimasa Takahashi, Koichi Watashi, and Kotaro Kiga

Subjects
Science
Abstract

Abstract Retrons are bacterial genetic elements that encode a reverse transcriptase and, in combination with toxic effector proteins, can serve as antiphage defense systems. However, the mechanisms of action of most retron effectors, and how phages evade retrons, are not well understood. Here, we show that some phages can evade retrons and other defense systems by producing specific tRNAs. We find that expression of retron-Eco7 effector proteins (PtuA and PtuB) leads to degradation of tRNATyr and abortive infection. The genomes of T5 phages that evade retron-Eco7 include a tRNA-rich region, including a highly expressed tRNATyr gene, which confers protection against retron-Eco7. Furthermore, we show that other phages (T1, T7) can use a similar strategy, expressing a tRNALys, to counteract a tRNA anticodon defense system (PrrC170).

Published
2024
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2. Development of CRISPR-Cas13a-based antimicrobials capable of sequence-specific killing of target bacteria

Author

Kotaro Kiga, Xin-Ee Tan, Rodrigo Ibarra-Chávez, Shinya Watanabe, Yoshifumi Aiba, Yusuke Sato’o, Feng-Yu Li, Teppei Sasahara, Bintao Cui, Moriyuki Kawauchi, Tanit Boonsiri, Kanate Thitiananpakorn, Yusuke Taki, Aa Haeruman Azam, Masato Suzuki, José R. Penadés, and Longzhu Cui

Subjects
Science
Abstract

CRISPR technology is emerging as a potential antimicrobial against antimicrobial-resistant bacteria. Here the authors develop a bacteriophage delivered Cas13a system for killing target bacteria and detecting bacterial genes.

Published
2020
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3. Development of CRISPR-Cas13a-based antimicrobials capable of sequence-specific killing of target bacteria

Author

Aa Haeruman Azam, Xin Ee Tan, Kotaro Kiga, Tanit Boonsiri, Kanate Thitiananpakorn, Yusuke Taki, José R. Penadés, Bintao Cui, Moriyuki Kawauchi, Yoshifumi Aiba, Yusuke Sato’o, Shinya Watanabe, Feng-Yu Li, Rodrigo Ibarra-Chávez, Longzhu Cui, Masato Suzuki, and Teppei Sasahara

Subjects
0301 basic medicine, Methicillin-Resistant Staphylococcus aureus, Staphylococcus aureus, CRISPR-Cas systems, Science, 030106 microbiology, General Physics and Astronomy, Microbial Sensitivity Tests, Biology, medicine.disease_cause, General Biochemistry, Genetics and Molecular Biology, Article, Microbiology, Bacteriophage, 03 medical and health sciences, Plasmid, Anti-Infective Agents, medicine, Escherichia coli, CRISPR, Bacteriophages, lcsh:Science, Clinical microbiology, Gene, Multidisciplinary, Cas9, Antimicrobials, Infectious-disease diagnostics, General Chemistry, Antimicrobial, biology.organism_classification, Anti-Bacterial Agents, 030104 developmental biology, lcsh:Q, Bacteria
Abstract

The emergence of antimicrobial-resistant bacteria is an increasingly serious threat to global health, necessitating the development of innovative antimicrobials. Here we report the development of a series of CRISPR-Cas13a-based antibacterial nucleocapsids, termed CapsidCas13a(s), capable of sequence-specific killing of carbapenem-resistant Escherichia coli and methicillin-resistant Staphylococcus aureus by recognizing corresponding antimicrobial resistance genes. CapsidCas13a constructs are generated by packaging programmed CRISPR-Cas13a into a bacteriophage capsid to target antimicrobial resistance genes. Contrary to Cas9-based antimicrobials that lack bacterial killing capacity when the target genes are located on a plasmid, the CapsidCas13a(s) exhibit strong bacterial killing activities upon recognizing target genes regardless of their location. Moreover, we also demonstrate that the CapsidCas13a(s) can be applied to detect bacterial genes through gene-specific depletion of bacteria without employing nucleic acid manipulation and optical visualization devices. Our data underscore the potential of CapsidCas13a(s) as both therapeutic agents against antimicrobial-resistant bacteria and nonchemical agents for detection of bacterial genes., CRISPR technology is emerging as a potential antimicrobial against antimicrobial-resistant bacteria. Here the authors develop a bacteriophage delivered Cas13a system for killing target bacteria and detecting bacterial genes.

Published
2019

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