1. Cortisone dissociates the Shaker family K+ channels from their beta subunits.
- Author
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Pan Y, Weng J, Kabaleeswaran V, Li H, Cao Y, Bhosle RC, and Zhou M
- Subjects
- Amino Acid Sequence, Animals, Binding Sites, Binding, Competitive drug effects, Cortisone chemistry, Crystallography, X-Ray, Humans, Kv1.2 Potassium Channel chemistry, Models, Molecular, Molecular Sequence Data, Molecular Structure, Protein Binding drug effects, Rats, Shaker Superfamily of Potassium Channels metabolism, Xenopus, Cortisone pharmacology, Kv1.2 Potassium Channel metabolism, Shaker Superfamily of Potassium Channels drug effects
- Abstract
The Shaker family voltage-dependent potassium channels (Kv1) are expressed in a wide variety of cells and are essential for cellular excitability. In humans, loss-of-function mutations of Kv1 channels lead to hyperexcitability and are directly linked to episodic ataxia and atrial fibrillation. All Kv1 channels assemble with beta subunits (Kv betas), and certain Kv betas, for example Kv beta 1, have an N-terminal segment that closes the channel by the N-type inactivation mechanism. In principle, dissociation of Kv beta 1, although never reported, should eliminate inactivation and thus potentiate Kv1 current. We found that cortisone increases rat Kv1 channel activity by binding to Kv beta 1. A crystal structure of the Kv beta-cortisone complex was solved to 1.82-A resolution and revealed novel cortisone binding sites. Further studies demonstrated that cortisone promotes dissociation of Kv beta. The new mode of channel modulation may be explored by native or synthetic ligands to fine-tune cellular excitability.
- Published
- 2008
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