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Your search keyword '"de Herreros, Antonio Garcia"' showing total 2 results
Start Over Author "de Herreros, Antonio Garcia" Journal nature cell biology
2 results on '"de Herreros, Antonio Garcia"'

1. A SNAIL1–SMAD3/4 transcriptional repressor complex promotes TGF-β mediated epithelial–mesenchymal transition

Author

Vincent, Theresa, primary, Neve, Etienne P. A., additional, Johnson, Jill R., additional, Kukalev, Alexander, additional, Rojo, Federico, additional, Albanell, Joan, additional, Pietras, Kristian, additional, Virtanen, Ismo, additional, Philipson, Lennart, additional, Leopold, Philip L., additional, Crystal, Ronald G., additional, de Herreros, Antonio Garcia, additional, Moustakas, Aristidis, additional, Pettersson, Ralf F., additional, and Fuxe, Jonas, additional

Published
2009
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2. A SNAIL1-SMAD3/4 transcriptional repressor complex promotes TGF-beta mediated epithelial-mesenchymal transition.

Author

Vincent T, Neve EP, Johnson JR, Kukalev A, Rojo F, Albanell J, Pietras K, Virtanen I, Philipson L, Leopold PL, Crystal RG, de Herreros AG, Moustakas A, Pettersson RF, and Fuxe J

Subjects
Animals, Blotting, Western, Cadherins genetics, Cell Line, Transformed, Cell Nucleus metabolism, Chromatin Immunoprecipitation, Epithelial Cells drug effects, Epithelial Cells metabolism, Epithelial Cells pathology, Humans, Intercellular Junctions metabolism, Mammary Neoplasms, Experimental genetics, Mammary Neoplasms, Experimental metabolism, Mammary Neoplasms, Experimental pathology, Mesoderm drug effects, Mesoderm metabolism, Mesoderm pathology, Mice, Mice, Inbred BALB C, Microscopy, Fluorescence, Promoter Regions, Genetic genetics, Protein Binding, Reverse Transcriptase Polymerase Chain Reaction, Smad3 Protein genetics, Smad4 Protein genetics, Snail Family Transcription Factors, Transcription Factors genetics, Tumor Cells, Cultured, Smad3 Protein metabolism, Smad4 Protein metabolism, Transcription Factors metabolism, Transforming Growth Factor beta pharmacology
Abstract

Epithelial-mesenchymal transition (EMT) is essential for organogenesis and is triggered during carcinoma progression to an invasive state. Transforming growth factor-beta (TGF-beta) cooperates with signalling pathways, such as Ras and Wnt, to induce EMT, but the molecular mechanisms are not clear. Here, we report that SMAD3 and SMAD4 interact and form a complex with SNAIL1, a transcriptional repressor and promoter of EMT. The SNAIL1-SMAD3/4 complex was targeted to the gene promoters of CAR, a tight-junction protein, and E-cadherin during TGF-beta-driven EMT in breast epithelial cells. SNAIL1 and SMAD3/4 acted as co-repressors of CAR, occludin, claudin-3 and E-cadherin promoters in transfected cells. Conversely, co-silencing of SNAIL1 and SMAD4 by siRNA inhibited repression of CAR and occludin during EMT. Moreover, loss of CAR and E-cadherin correlated with nuclear co-expression of SNAIL1 and SMAD3/4 in a mouse model of breast carcinoma and at the invasive fronts of human breast cancer. We propose that activation of a SNAIL1-SMAD3/4 transcriptional complex represents a mechanism of gene repression during EMT.

Published
2009
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