1. Evaluating totipotency using criteria of increasing stringency.
- Author
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Posfai E, Schell JP, Janiszewski A, Rovic I, Murray A, Bradshaw B, Yamakawa T, Pardon T, El Bakkali M, Talon I, De Geest N, Kumar P, To SK, Petropoulos S, Jurisicova A, Pasque V, Lanner F, and Rossant J
- Subjects
- Animals, Blastomeres metabolism, Embryo, Mammalian metabolism, Embryonic Stem Cells metabolism, Female, Gene Expression Profiling, Gene Regulatory Networks, Male, Mice, Pluripotent Stem Cells metabolism, Single-Cell Analysis, Totipotent Stem Cells metabolism, Blastomeres cytology, Cell Differentiation, Cell Lineage genetics, Embryo, Mammalian cytology, Embryonic Stem Cells cytology, Pluripotent Stem Cells cytology, Totipotent Stem Cells cytology
- Abstract
Totipotency is the ability of a single cell to give rise to all of the differentiated cell types that build the conceptus, yet how to capture this property in vitro remains incompletely understood. Defining totipotency relies on a variety of assays of variable stringency. Here, we describe criteria to define totipotency. We explain how distinct criteria of increasing stringency can be used to judge totipotency by evaluating candidate totipotent cell types in mice, including early blastomeres and expanded or extended pluripotent stem cells. Our data challenge the notion that expanded or extended pluripotent states harbour increased totipotent potential relative to conventional embryonic stem cells under in vitro and in vivo conditions.
- Published
- 2021
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