1. Fast and highly sensitive full-length single-cell RNA sequencing using FLASH-seq
- Author
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Vincent Hahaut, Dinko Pavlinic, Walter Carbone, Sven Schuierer, Pierre Balmer, Mathieu Quinodoz, Magdalena Renner, Guglielmo Roma, Cameron S. Cowan, and Simone Picelli
- Subjects
Sequence Analysis, RNA ,Gene Expression Profiling ,Exome Sequencing ,Biomedical Engineering ,Molecular Medicine ,RNA ,Bioengineering ,Single-Cell Analysis ,Applied Microbiology and Biotechnology ,Biotechnology - Abstract
We present FLASH-seq (FS), a full-length single-cell RNA sequencing (scRNA-seq) method with increased sensitivity and reduced hands-on time compared to Smart-seq3. The entire FS protocol can be performed in ~4.5 hours, is simple to automate and can be easily miniaturized to decrease resource consumption. The FS protocol can also use unique molecular identifiers (UMIs) for molecule counting while displaying reduced strand-invasion artifacts. FS will be especially useful for characterizing gene expression at high resolution across multiple samples.
- Published
- 2021