1. Super-resolution RNA imaging using a rhodamine-binding aptamer with fast exchange kinetics
- Author
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Jens Lackner, Karin Nienhaus, Daniel Englert, Annabell Martin, Andres Jäschke, Murat Sunbul, Benjamin Hacene, Franziska Grün, and G. Ulrich Nienhaus
- Subjects
Aptamer ,Biomedical Engineering ,Bioengineering ,Applied Microbiology and Biotechnology ,Rhodamine ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Microscopy ,Fluorescent Dyes ,030304 developmental biology ,0303 health sciences ,Rhodamines ,Chemistry ,Resolution (electron density) ,RNA ,Aptamers, Nucleotide ,Exchange kinetics ,Fluorescence ,Photobleaching ,Kinetics ,Microscopy, Fluorescence ,Biophysics ,Molecular Medicine ,030217 neurology & neurosurgery ,Biotechnology - Abstract
Overcoming limitations of previous fluorescent light-up RNA aptamers for super-resolution imaging, we present RhoBAST, an aptamer that binds a fluorogenic rhodamine dye with fast association and dissociation kinetics. Its intermittent fluorescence emission enables single-molecule localization microscopy with a resolution not limited by photobleaching. We use RhoBAST to image subcellular structures of RNA in live and fixed cells with about 10-nm localization precision and a high signal-to-noise ratio.
- Published
- 2021
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