1. Functional Interrogation and Mining of Natively-Paired Human VH:VL Antibody Repertoires
- Author
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M. Anthony Moody, Aaron G. Schmidt, John Misasi, Wing Pui Kong, Bo Wang, Farida Laboune, John R. Mascola, George Georgiou, Chang W. Choi, Alberto Cagigi, Eun Sung Yang, Nancy J. Sullivan, Morgan R. Timm, Kwanyee Leung, Daniel C. Douek, Rui Kong, Julie E. Ledgerwood, Ahmed S. Fahad, Barney S. Graham, David R. Ambrozak, Jonathan R. McDaniel, Elise G. Viox, Erica Normandin, Brandon J. DeKosky, Jiwon Lee, Thomas Niezold, George Delidakis, Amy R. Henry, Aurélie Ploquin, Mark Connors, Leigh Kendra Elizabeth, Andrew D. Ellington, and William N. Voss
- Subjects
0301 basic medicine ,Biomedical Engineering ,Hemagglutinin (influenza) ,Bioengineering ,HIV Infections ,Yeast display ,HIV Antibodies ,medicine.disease_cause ,Applied Microbiology and Biotechnology ,Article ,03 medical and health sciences ,0302 clinical medicine ,Peptide Library ,medicine ,Humans ,Amino Acid Sequence ,Peptide library ,chemistry.chemical_classification ,B-Lymphocytes ,Ebola virus ,biology ,virus diseases ,Antibodies, Monoclonal ,High-Throughput Nucleotide Sequencing ,Amplicon ,Virology ,Antibodies, Neutralizing ,3. Good health ,030104 developmental biology ,chemistry ,030220 oncology & carcinogenesis ,biology.protein ,HIV-1 ,Molecular Medicine ,Surface expression ,Antibody ,Glycoprotein ,Biotechnology - Abstract
We present a technology to screen millions of B cells for natively paired human antibody repertoires. Libraries of natively paired, variable region heavy and light (VH:VL) amplicons are expressed in a yeast display platform that is optimized for human Fab surface expression. Using our method we identify HIV-1 broadly neutralizing antibodies (bNAbs) from an HIV-1 slow progressor and high-affinity neutralizing antibodies against Ebola virus glycoprotein and influenza hemagglutinin.
- Published
- 2018